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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.4%
Recovery was determined by spiking various levels of 2,3-BPG into serum and plasma .
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
96 |
94-102 |
Plasma |
97 |
93-99 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 2,3-BPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
93-98% |
93-101% |
90-96% |
96-103% |
EDTA plasma(n=5) |
97-102% |
102-107% |
92-97% |
93-95% |
heparin plasma(n=5) |
95-100%
|
95-99% |
98-104% |
86-95% |
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