AMELX ELISA Kit for Human(Homo sapiens) | EIAab.comAMELX ELISA Kit for Human(Homo sapiens)

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Index > Protein center > AMELX(Gene name) > Human

AMELX (Gene name),

Amelogenin, X isoform (Protein name ), AMELX_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

AMELX(AMG;AMGX);

Protein name:

Amelogenin, X isoform;

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

Subcellular Location:

Secreted extracellular space extracellular matrix

Protein Attributes:

Sequence length:

191

Sequence:

50:

100:

150:

191:

PLPPQPPLPP | MFPMQPLPPM | LPDLTLEAWP | STDKTKREEV | D

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q99217

MIM:

300391

Pfam:

PF02948

STRING:

9606.ENSP00000370088

UniGene:

Hs.654436

KEGG:

hsa:265

Uniprot:

Q99217

FOR

ELISA Kit for Human Amelogenin, X isoform

Cat.:

E14172h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Human Amelogenin, X isoform

Cat.:

E14172m

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Human Amelogenin, X isoform

Cat.:

E14172r

Price:

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Manual:

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Packing:

96T

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ELISA Kit for Human Amelogenin, X isoform

MSDS:

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CLIA Kit for Human Amelogenin, X isoform

MSDS:

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CLIA Kit for Human Amelogenin, X isoform

Cat.:

U14172r

Price:

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Manual:

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Packing:

96T

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CLIA Kit for Human Amelogenin, X isoform

Cat.:

U14172h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Human Amelogenin, X isoform

Cat.:

U14172m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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Polyclonal Antibody for Human Amelogenin, X isoform

Cat.:

P14172Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Amelogenin, X isoform

Monoclonal Antibody for Human Amelogenin, X isoform

Protein for Human Amelogenin, X isoform

Cat.:

R14172h

Price:

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Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	97-108
Plasma	103	95-106

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-104
Plasma	99	93-107

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	100	98-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMELX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-112%	97-109%	95-111%	99-107%
EDTA plasma(n=5)	99-105%	100-107%	97-109%	94-112%
heparin plasma(n=5)	95-110%	98-106%	96-111%	98-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-106%	92-102%	91-104%	94-102%
EDTA plasma(n=5)	91-107%	95-103%	92-107%	95-103%
heparin plasma(n=5)	93-104%	94-106%	96-104%	92-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-102%	93-105%	94-103%	97-102%
EDTA plasma(n=5)	94-106%	92-107%	96-104%	93-107%
heparin plasma(n=5)	95-104%	98-102%	95-103%	96-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes."
Salido E.C. , Yen P.H. , Koprivnikar K. , Yu L.-C. , Shapiro L.J.
Am. J. Hum. Genet.50:303-316(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Tooth bud.
2.

"A nomenclature for X-linked amelogenesis imperfecta."
Hart P.S. , Hart T.C. , Simmer J.P. , Wright J.T.
Arch. Oral Biol.47:255-260(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3)
3.

"The DNA sequence of the human X chromosome."
Ross M.T. , Grafham D.V. , Coffey A.J. , Scherer S. , McLay K. , Muzny D. , Platzer M. , Howell G.R. , Burrows C. , Bird C.P. , Frankish A. , Lovell F.L. , Howe K.L. , Ashurst J.L. , Fulton R.S. , Sudbrak R. , Wen G. , Jones M.C. , more...
Nature434:325-337(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
5.

"Human amelogenins: sequences of 'TRAP' molecules."
Fincham A.G. , Hu Y. , Pavlova Z. , Slavkin H.C. , Snead M.L.
Calcif. Tissue Int.45:243-250(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 17-60
6.

"Production of a monoclonal antibody against human amelogenin."
Catalano-Sherman J. , Laskov R. , Palmon A. , David S. , Deutsch D.
Calcif. Tissue Int.54:76-80(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-37
7.

"A human X-Y homologous region encodes 'amelogenin'."
Nakahori Y. , Takenaka O. , Nakagome Y.
Genomics9:264-269(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-191 (ISOFORM 1)
8.

"Amino acid sequence of a major human amelogenin protein employing Edman degradation and cDNA sequencing."
Catalano-Sherman J. , Palmon A. , Burstein Y. , Deutsch D.
J. Dent. Res.72:1566-1572(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-190;PARTIAL PROTEIN SEQUENCE
tissue: Tooth bud.
9.

"Amelogenin signal peptide mutation: correlation between mutations in the amelogenin gene (AMGX) and manifestations of X-linked amelogenesis imperfecta."
Lagerstroem-Fermer M. , Nilddon M. , Baeckman B. , Salido E. , Shapiro L. , Pettersson U. , Landergren U.
Genomics26:159-162(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT AI1E 5-ILE--ALA-8 DELINS THR
10.

"Characterisation of molecular defects in X-linked amelogenesis imperfecta (AIH1)."
Lench N.J. , Winter G.B.
Hum. Mutat.5:251-259(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT AI1E ILE-37
11.

"Mutational analysis of X-linked amelogenesis imperfecta in multiple families."
Hart S. , Hart T. , Gibson C. , Wright J.T.
Arch. Oral Biol.45:79-86(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT AI1E THR-56
12.

"An amelogenin gene defect associated with human X-linked amelogenesis imperfecta."
Collier P.M. , Sauk J.J. , Rosenbloom S.J. , Yuan Z.A. , Gibson C.W.
Arch. Oral Biol.42:235-242(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT AI1E THR-56
13.

"Amelogenin p.M1T and p.W4S mutations underlying hypoplastic X-linked amelogenesis imperfecta."
Kim J.-W. , Simmer J.P. , Hu Y.Y. , Lin B.P.-L. , Boyd C. , Wright J.T. , Yamada C.J.M. , Rayes S.K. , Feigal R.J. , Hu J.C.-C.
J. Dent. Res.83:378-383(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT AI1E SER-4

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