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Index > Protein center > AMY1A(Gene name) > Human
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  • AMY1A (Gene name),
  • Alpha-amylase 1 (Protein name ),  AMY1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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    • Gene name:
    AMY1A(AMY1);
    Protein name:
    Alpha-amylase 1;
    Alternative:
    Salivary alpha-amylase;1,4-alpha-D-glucan glucanohydrolase 1;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    511
    Sequence:
    50:
    MKLFWLLFTI | GFCWAQYSSN | TQQGRTSIVH | LFEWRWVDIA | LECERYLAPK | 
    100:
    GFGGVQVSPP | NENVAIHNPF | RPWWERYQPV | SYKLCTRSGN | EDEFRNMVTR | 
    150:
    CNNVGVRIYV | DAVINHMCGN | AVSAGTSSTC | GSYFNPGSRD | FPAVPYSGWD | 
    200:
    FNDGKCKTGS | GDIENYNDAT | QVRDCRLSGL | LDLALGKDYV | RSKIAEYMNH | 
    250:
    LIDIGVAGFR | IDASKHMWPG | DIKAILDKLH | NLNSNWFPEG | SKPFIYQEVI | 
    300:
    DLGGEPIKSS | DYFGNGRVTE | FKYGAKLGTV | IRKWNGEKMS | YLKNWGEGWG | 
    350:
    FMPSDRALVF | VDNHDNQRGH | GAGGASILTF | WDARLYKMAV | GFMLAHPYGF | 
    400:
    TRVMSSYRWP | RYFENGKDVN | DWVGPPNDNG | VTKEVTINPD | TTCGNDWVCE | 
    450:
    HRWRQIRNMV | NFRNVVDGQP | FTNWYDNGSN | QVAFGRGNRG | FIVFNNDDWT | 
    500:
    FSLTLQTGLP | AGTYCDVISG | DKINGNCTGI | KIYVSDDGKA | HFSISNSAED | 
    511:
    PFIAIHAESK | L
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    P04745
    UniGene:
    Hs.599274
    Pfam:
    PF00128
    SMR:
    P04745
    KEGG:
    hsa:276
    STRING:
    9606.ENSP00000330484
    MIM:
    104700
    Uniprot:
    P04745
     
    FOR
    ELISA Kit for Human Alpha-amylase 1
    Cat.:
    E1482h
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Alpha-amylase 1
    Cat.:
    U1482h
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    Polyclonal Antibody for Human Alpha-amylase 1
    Cat.:
    P1482Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Alpha-amylase 1
    Protein for Human Alpha-amylase 1

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.2

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.1

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of Alpha-amylase 1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    96

    92-103

    Plasma

    89

    79-95
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-amylase 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    89-98%

    93-105%

    95-103%

    92-105%

    EDTA plasma(n=5)

    95-103%

    90-99%

    89-95%

    84-97%

    heparin plasma(n=5)

    85-94%

     

    85-97%

    88-101%

    92-101%
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Primary structure of human salivary alpha-amylase gene."
      Nishide T. , Nakamura Y. , Emi M. , Yamamoto T. , Ogawa M. , Mori T. , Matsubara K.
      Gene41:299-304(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Corrected sequences of cDNAs for human salivary and pancreatic alpha-amylases."
      Nakamura Y. , Ogawa M. , Nishide T. , Emi M. , Kosaki G. , Himeno S. , Matsubara K.
      Gene28:263-270(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM;SEQUENCE REVISION
    4. 4.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Thyroid.
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56 AND 408-448
    8. 8.
      "Identification of a human salivary amylase gene. Partial sequence of genomic DNA suggests a mode of regulation different from that of mouse, Amy1."
      Handy D.E. , Larsen S.H. , Karn R.C. , Hodes M.E.
      Mol. Biol. Med.4:145-155(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-293 AND 450-511
    9. 9.
      "Electrophoretic characterization of posttranslational modifications of human parotid salivary alpha-amylase."
      Bank R.A. , Hettema E.H. , Arwert F. , Amerongen A.V. , Pronk J.C.
      Electrophoresis12:74-79(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: POST-TRANSLATIONAL MODIFICATIONS
    10. 10.
      "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
      J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-427
      tissue: Saliva.
    11. 11.
      "Structure of human salivary alpha-amylase at 1.6-A resolution: implications for its role in the oral cavity."
      Ramasubbu N. , Paloth V. , Luo Y. , Brayer G.D. , Levine M.J.
      Acta Crystallogr. D52:435-446(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
    12. 12.
      "Probing the role of a mobile loop in substrate binding and enzyme activity of human salivary amylase."
      Ramasubbu N. , Ragunath C. , Mishra P.J.
      J. Mol. Biol.325:1061-1076(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF WILD-TYPE AND MUTANT 321-GLY--ALA-325 DEL
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