Angiogenin (Protein name
), ANGI_PIG from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
ANG;
Protein name:
Angiogenin;
Alternative:
Ribonuclease 5(RNase 5);
Organism:
Pig (Sus scrofa).
General Annotation
Sub Unit:
Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.
Function:
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 5.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 9.2%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
95
91-101
Plasma
94
90-99
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
89-99%
91-102%
93-103%
90-104%
EDTA plasma(n=5)
89-98%
90-103%
91-97%
92-102%
heparin plasma(n=5)
90-101%
92-105%
94-107%
91-106%
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