AXIN1 ELISA Kit for Human(Homo sapiens) | EIAab.comAXIN1 ELISA Kit for Human(Homo sapiens)

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AXIN1 (Gene name),

Axin-1 (Protein name ), AXIN1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

AXIN1(AXIN);

Protein name:

Axin-1;

Alternative:

Axis inhibition protein 1(hAxin);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer (By similarity). Component of the beta-catenin destruction complex, containing at least, CTNNB1, an axin and GSK3B, that regulates CTNNB1 protein levels through phosphorylation and ubiquitination. Interacts with CTNNB1 (via the armadillo repeats 2-7). Interacts with GSK3B; the interaction hyperphosphorylates CTNNB1 leading to its ubiquitination and destruction. Component of the AXIN1-HIPK2-TP53 complex. Interacts directly in the complex with TP53 and HIPK2. Interacts with DAXX; the interaction stimulates the interaction of DAXX with TP53, stimulates 'Ser-46' phosphorylation of TP53 and induces cell death on UV irradiation. Also binds APC, SMAD6, SMAD7 and RNF111. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4. Interacts with ANKRD6 and AIDA (By similarity). Interacts with MDFI; the interaction decreases AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with MDFIC; the interaction inhibits beta-cateninin-mediated signaling and AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with LRP5 (via its phosphorylated PPPSP motifs); the interaction is stimulated by WNT1 and GSK3B and activates beta-catenin signaling. Interacts (via the C-terminal) with PPP1CA; the interaction dephosphorylates AXIN1 and regulates interaction with GSK3B. Interacts with PPP2CA; the interaction dephosphorylates AXIN1. Interacts with MACF1 (By similarity). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Function:

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.

Subcellular Location:

Cytoplasm Nucleus Cell membrane MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX.

Protein Attributes:

Sequence length:

862

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

862:

EEKIIGKVEK | VD

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:8312

Pfam:

PF08833

UniGene:

Hs.592082

SMR:

O15169

MIM:

114550

STRING:

9606.ENSP00000262320

String:

O15169

Uniprot:

O15169

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ELISA Kit for Human Axin-1

Cat.:

E9657h

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Packing:

96T

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ELISA Kit for Human Axin-1

MSDS:

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ELISA Kit for Human Axin-1

Cat.:

E9657m

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96T

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ELISA Kit for Human Axin-1

Cat.:

E9657r

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96T

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CLIA Kit for Human Axin-1

Cat.:

U9657m

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MSDS:

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Packing:

96T

CLIA Kit for Human Axin-1

Cat.:

U9657r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human Axin-1

MSDS:

Please sign in first.

CLIA Kit for Human Axin-1

Cat.:

U9657h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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Polyclonal Antibody for Human Axin-1

Cat.:

P9657Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Axin-1

Cat.:

P9657Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Axin-1

Protein for Human Axin-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation."
Zeng L. , Fagotto F. , Zhang T. , Hsu W. , Vasicek T.J. , Perry W.L. III , Lee J.J. , Tilghman S.M. , Gumbiner B.M. , Costantini F.
Cell90:181-192(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
Daniels R.J. , Peden J.F. , Lloyd C. , Horsley S.W. , Clark K. , Tufarelli C. , Kearney L. , Buckle V.J. , Doggett N.A. , Flint J. , Higgs D.R.
Hum. Mol. Genet.10:339-352(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
3.

"The sequence and analysis of duplication-rich human chromosome 16."
Martin J. , Han C. , Gordon L.A. , Terry A. , Prabhakar S. , She X. , Xie G. , Hellsten U. , Chan Y.M. , Altherr M. , Couronne O. , Aerts A. , Bajorek E. , Black S. , Blumer H. , Branscomb E. , Brown N.C. , Bruno W.J. , more...
Nature432:988-994(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 471-862 (ISOFORM 1)
tissue: Lymphoma.
tissue: Renal cell carcinoma.
5.

"Identification of a domain of Axin that binds to the serine/threonine protein phosphatase 2A and a self-binding domain."
Hsu W. , Zeng L. , Costantini F.
J. Biol. Chem.274:3439-3445(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GSK3B AND PPP2CA;PHOSPHORYLATION;DEPHOSPHORYLATION
6.

"AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1."
Satoh S. , Daigo Y. , Furukawa Y. , Kato T. , Miwa N. , Nishiwaki T. , Kawasoe T. , Ishiguro H. , Fujita M. , Tokino T. , Sasaki Y. , Imaoka S. , Murata M. , Shimano T. , Yamaoka Y. , Nakamura Y.
Nat. Genet.24:245-250(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISEASE
7.

"Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway."
Mao J. , Wang J. , Liu B. , Pan W. , Farr G.H. III , Flynn C. , Yuan H. , Takada S. , Kimelman D. , Li L. , Wu D.
Mol. Cell7:801-809(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LRP5
8.

"I-mfa domain proteins interact with Axin and affect its regulation of the Wnt and c-Jun N-terminal kinase signaling pathways."
Kusano S. , Raab-Traub N.
Mol. Cell. Biol.22:6393-6405(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MDFI AND MDFIC;FUNCTION
9.

"Regulation of the Wnt signaling pathway by disabled-2 (Dab2)."
Howe P.H.
EMBO J.22:3084-3094(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DAB2
10.

"The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through distinct mechanisms."
Wong C.K. , Luo W. , Deng Y. , Zou H. , Ye Z. , Lin S.-C.
J. Biol. Chem.279:39366-39373(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DIXDC1; MAP3K1 AND MAP3K4
11.

"Increased DNA methylation at the AXIN1 gene in a monozygotic twin from a pair discordant for a caudal duplication anomaly."
Oates N.A. , van Vliet J. , Duffy D.L. , Kroes H.Y. , Martin N.G. , Boomsma D.I. , Campbell M. , Coulthard M.G. , Whitelaw E. , Chong S.
Am. J. Hum. Genet.79:155-162(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN CAUDAL DUPLICATION ANOMALY
12.

"Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia."
Liu W. , Rui H. , Wang J. , Lin S. , He Y. , Chen M. , Li Q. , Ye Z. , Zhang S. , Chan S.C. , Chen Y.-G. , Han J. , Lin S.-C.
EMBO J.25:1646-1658(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SMAD6; SMAD7 AND RNF111;FUNCTION;SUBCELLULAR LOCATION
13.

"Daxx cooperates with the Axin/HIPK2/p53 complex to induce cell death."
Li Q. , Wang X. , Wu X. , Rui Y. , Liu W. , Wang J. , Wang X. , Liou Y.C. , Ye Z. , Lin S.C.
Cancer Res.67:66-74(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DAXX;IDENTIFICATION AS A COMPONENT OF THE AXIN1-HIPK2-TP53 COMPLEX;SUBCELLULAR LOCATION;FUNCTION
14.

"Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex."
Luo W. , Peterson A. , Garcia B.A. , Coombs G. , Kofahl B. , Heinrich R. , Shabanowitz J. , Hunt D.F. , Yost H.J. , Virshup D.M.
EMBO J.26:1511-1521(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-75; SER-77; SER-217 AND SER-469;INTERACTION WITH GSK3B AND PPP1CA;IDENTIFICATION BY MASS SPECTROMETRY
15.

"SUMOylation target sites at the C terminus protect Axin from ubiquitination and confer protein stability."
Kim M.J. , Chia I.V. , Costantini F.
FASEB J.22:3785-3794(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION
16.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling."
Huang S.M. , Mishina Y.M. , Liu S. , Cheung A. , Stegmeier F. , Michaud G.A. , Charlat O. , Wiellette E. , Zhang Y. , Wiessner S. , Hild M. , Shi X. , Wilson C.J. , Mickanin C. , Myer V. , Fazal A. , Tomlinson R. , Serluca F. , more...
Nature461:614-620(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ADP-RIBOSYLATION;UBIQUITINATION;DOMAIN TANKYRASE-BINDING MOTIF;INTERACTION WITH TNKS AND TNKS2
18.

"The Ubiquitin specific protease USP34 regulates Axin stability and Wnt/beta-catenin signaling."
Lui T.T. , Lacroix C. , Ahmed S.M. , Goldenberg S.J. , Leach C.A. , Daulat A.M. , Angers S.
Mol. Cell. Biol.31:2053-2065(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;DEUBIQUITINATION BY USP34;SUBCELLULAR LOCATION
19.

"RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling."
Zhang Y. , Liu S. , Mickanin C. , Feng Y. , Charlat O. , Michaud G.A. , Schirle M. , Shi X. , Hild M. , Bauer A. , Myer V.E. , Finan P.M. , Porter J.A. , Huang S.M. , Cong F.
Nat. Cell Biol.13:623-629(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ADP-RIBOSYLATION;UBIQUITINATION;INTERACTION WITH RNF146; TNKS AND TNKS2
20.

"Ubiquitin ligase RNF146 regulates tankyrase and Axin to promote Wnt signaling."
Callow M.G. , Tran H. , Phu L. , Lau T. , Lee J. , Sandoval W.N. , Liu P.S. , Bheddah S. , Tao J. , Lill J.R. , Hongo J.A. , Davis D. , Kirkpatrick D.S. , Polakis P. , Costa M.
PLoS ONE6:E22595-E22595(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TNKS;UBIQUITINATION
21.

"Structural basis of the axin-adenomatous polyposis coli interaction."
Spink K.E. , Polakis P. , Weis W.I.
EMBO J.19:2270-2279(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS) OF 74-220 IN COMPLEX WITH APC
22.

"Structural basis for recruitment of glycogen synthase kinase 3beta to the axin-APC scaffold complex."
Dajani R. , Fraser E. , Roe S.M. , Yeo M. , Good V.M. , Thompson V. , Dale T.C. , Pearl L.H.
EMBO J.22:494-501(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 383-400 IN COMPLEX WITH GSK3B
23.

"Mutational spectrum of beta-catenin, AXIN1, and AXIN2 in hepatocellular carcinomas and hepatoblastomas."
Taniguchi K. , Roberts L.R. , Aderca I.N. , Dong X. , Qian C. , Murphy L.M. , Nagorney D.M. , Burgart L.J. , Roche P.C. , Smith D.I. , Ross J.A. , Liu W.
Oncogene21:4863-4871(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HCC ARG-106; LEU-345; SER-425 AND SER-650;VARIANT HEPATOBLASTOMA GLN-841

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