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s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.4%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
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Recovery
Recovery was determined by spiking various levels of BDNF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
93
91-98
Plasma
94
93-100
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Recovery was determined by spiking various levels of BDNF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
94-103
Plasma
98
96-100
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-96%
90-100%
95-99%
92-96%
EDTA plasma(n=5)
96-103%
93-96%
96-98%
93-102%
heparin plasma(n=5)
89-104%
91-96%
89-92%
87-97%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
96-101%
93-99%
95-105%
92-96%
EDTA plasma(n=5)
99-104%
94-106%
99-110%
96-99%
heparin plasma(n=5)
95-102%
97-102%
101-109%
91-96%
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Vaild Sequence:
