Histo-blood group ABO system transferase (Protein name
), BGAT_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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Predicted Eptitope
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Gene name:
ABO;
Protein name:
Histo-blood group ABO system transferase;
Alternative:
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase;Fucosylglycoprotein 3-alpha-galactosyltransferase;2.4.1.37;Glycoprotein-fucosylgalactoside alpha-galactosyltransferase);2.4.1.40;Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase);Histo-blood group B transferase(B transferase);Histo-blood group A transferase(A transferase);NAGAT;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
N/A
Function:
This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
Subcellular Location:
Golgi apparatus
Golgi stack membrane
Single-pass type II membrane protein
Secreted
Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Polyclonal Antibody for Human Histo-blood group ABO system transferase
Monoclonal Antibody for Human Histo-blood group ABO system transferase
Monoclonal Antibody for Human Histo-blood group ABO system transferase
Monoclonal Antibody for Human Histo-blood group ABO system transferase
Protein for Human Histo-blood group ABO system transferase
Protein for Human Histo-blood group ABO system transferase
Protein for Human Histo-blood group ABO system transferase
R&D Technical Data
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.8%
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Recovery
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Recovery was determined by spiking various levels of BGAT into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
94
89-101
Plasma
95
91-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BGAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-102%
90-100%
85-103%
91-98%
EDTA plasma(n=5)
90-101%
89-101%
87-102%
90-102%
heparin plasma(n=5)
93-99%
91-100%
92-99%
88-104%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-156
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Cited for: ERRATUM
5.
"Human histo-blood group ABO gene locus alleles." Yamamoto F.
Submitted (1999-03) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];VARIANTS LEU-156; GLY-176; SER-235 AND MET-266
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ARG-230; SER-235; MET-266 AND ALA-268
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS GLY-176; SER-235; MET-266 AND ALA-268
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-354;VARIANTS SER-74; LEU-156; MET-163; GLY-176; SER-235; MET-266; ALA-268; ARG-268; ARG-288 AND MET-346
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-354;VARIANTS LEU-156; GLY-176; ARG-214; ILE-216; ASP-223; SER-235; MET-266; ALA-268; MET-277; ASN-291; GLY-352 AND TRP-352
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-354;VARIANTS GLY-176; SER-235; MET-266 AND ALA-268
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 126-354;VARIANTS GLY-176; SER-235 AND MET-346
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 146-334;VARIANTS MET-266 AND ALA-268
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Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 64-354;METAL-BINDING;MUTAGENESIS OF GLU-303
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Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 64-345 OF MUTANT SER-234 IN COMPLEX WITH GLYCOPROTEIN FUCOSYL-GALACTOSE ANALOG
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Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 64-345 IN COMPLEXES WITH UDP; UDP-N-ACETYL-GALACTOSAMINE; UDP-GALACTOSE AND GLYCOPROTEIN FUCOSYL-GALACTOSE ANALOGS
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Cited for: X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS) OF 64-354 OF MUTANTS SER-74 AND ARG-268 IN COMPLEXES WITH GLYCOPROTEIN FUCOSYL-GALACTOSE ANALOG AND UDP-N-ACETYL-GALACTOSAMINE
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Cited for: X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS) OF 64-354 OF HISTO-BLOOD GROUP GROUP A TRANSFERASE AND HISTO-BLOOD GROUP GROUP B TRANSFERASE IN COMPLEXES WITH GLYCOPROTEIN FUCOSYL-GALACTOSE ANALOGS
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Cited for: X-RAY CRYSTALLOGRAPHY (1.67 ANGSTROMS) OF 64-354 OF MUTANTS ARG-214 AND THR-214;COFACTOR;CHARACTERIZATION OF VARIANT ARG-214;MUTAGENESIS OF MET-214