Cathepsin B (Protein name
), CATB_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
CTSB(CPSB);
Protein name:
Cathepsin B;
Alternative:
Cathepsin B1;APP secretase(APPS);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2.
Function:
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Subcellular Location:
Lysosome
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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12% SDS-PAGE Analysis
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 5.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 8.3%
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Recovery
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
92-105
Plasma
96
93-104
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-101%
90-103%
93-105%
92-102%
EDTA plasma(n=5)
89-99%
93-101%
89-98%
90-105%
heparin plasma(n=5)
92-101%
94-105%
98-107%
95-108%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT VAL-26
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS VAL-26 AND GLY-53
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]