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Index > Protein center > CTSB(Gene name) > Human
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  • CTSB (Gene name),
  • Cathepsin B (Protein name ),  CATB_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (1)
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  • Gene name:
    CTSB(CPSB);
    Protein name:
    Cathepsin B;
    Alternative:
    Cathepsin B1;APP secretase(APPS);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2.
    Function:
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    Subcellular Location:
    Lysosome Melanosome Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    Protein Attributes:
    Sequence length:
    339
    Sequence:
    50:
    MWQLWASLCC | LLVLANARSR | PSFHPLSDEL | VNYVNKRNTT | WQAGHNFYNV | 
    100:
    DMSYLKRLCG | TFLGGPKPPQ | RVMFTEDLKL | PASFDAREQW | PQCPTIKEIR | 
    150:
    DQGSCGSCWA | FGAVEAISDR | ICIHTNAHVS | VEVSAEDLLT | CCGSMCGDGC | 
    200:
    NGGYPAEAWN | FWTRKGLVSG | GLYESHVGCR | PYSIPPCEHH | VNGSRPPCTG | 
    250:
    EGDTPKCSKI | CEPGYSPTYK | QDKHYGYNSY | SVSNSEKDIM | AEIYKNGPVE | 
    300:
    GAFSVYSDFL | LYKSGVYQHV | TGEMMGGHAI | RILGWGVENG | TPYWLVANSW | 
    339:
    NTDWGDNGFF | KILRGQDHCG | IESEVVAGIP | RTDQYWEKI
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    KEGG:
    SMR:
    Pfam:
    UniGene:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405m
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Cathepsin B
    Cat.:
    E0405c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Cathepsin B
    Cat.:
    U0405m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Cathepsin B
    Polyclonal Antibody for Human Cathepsin B
    Cat.:
    P0405Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Cathepsin B
    Cat.:
    P0405Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Cathepsin B
    Cat.:
    P0405Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Cathepsin B
    Polyclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Monoclonal Antibody for Human Cathepsin B
    Protein for Human Cathepsin B
    Protein for Human Cathepsin B
    Protein for Human Cathepsin B
    Protein for Human Cathepsin B
    Protein for Human Cathepsin B
    Protein for Human Cathepsin B

    R&D Technical Data
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           12% SDS-PAGE Analysis

     

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 5.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 8.3%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    92-105

    Plasma

    96

    93-104

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    88-101%

    90-103%

    93-105%

    92-102%

    EDTA plasma(n=5)

    89-99%

    93-101%

    89-98%

    90-105%

    heparin plasma(n=5)

    92-101%

     

    94-105%

    98-107%

    95-108%

     

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    References
    1. 1.
      "Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs."
      Chan S.J. , San Segundo B. , McCormick M.B. , Steiner D.F.
      Proc. Natl. Acad. Sci. U.S.A.83:7721-7725(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT VAL-26
    2. 2.
      "Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene."
      Cao L. , Taggart R.T. , Berquin I.M. , Moin K. , Fong D. , Sloane B.F.
      Gene139:163-169(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Gastric carcinoma.
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT VAL-26
      tissue: Esophagus.
    4. 4.
      "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T. , Ota T. , Nishikawa T. , Hayashi K. , Suzuki Y. , Yamamoto J. , Wakamatsu A. , Kimura K. , Sakamoto K. , Hatano N. , Kawai Y. , Ishii S. , Saito K. , Kojima S. , Sugiyama T. , Ono T. , Okano K. , Yoshikawa Y. , more...
      DNA Res.12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS VAL-26 AND GLY-53
      tissue: Brain.
      tissue: Placenta.
    7. 7.
      "Amino acid sequence of human liver cathepsin B."
      Ritonja A. , Popovic T. , Turk V. , Wiedenmann K. , Machleidt W.
      FEBS Lett.181:169-172(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 80-126 AND 129-333
      tissue: Liver.
    8. 8.
      "Human tumour cathepsin B. Comparison with normal liver cathepsin B."
      Moin K. , Day N.A. , Sameni M. , Hasnain S. , Hirama T. , Sloane B.F.
      Biochem. J.285:427-434(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 80-91 AND 129-139
      tissue: Liver.
    9. 9.
      "Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B."
      Fong D. , Calhoun D.H. , Hsieh W.-T. , Lee B. , Wells R.D.
      Proc. Natl. Acad. Sci. U.S.A.83:2909-2913(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 131-339
    10. 10.
      "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins."
      Basrur V. , Yang F. , Kushimoto T. , Higashimoto Y. , Yasumoto K. , Valencia J. , Muller J. , Vieira W.D. , Watabe H. , Shabanowitz J. , Hearing V.J. , Hunt D.F. , Appella E.
      J. Proteome Res.2:69-79(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]
      tissue: Melanoma.
    11. 11.
      "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
      Chi A. , Valencia J.C. , Hu Z.-Z. , Watabe H. , Yamaguchi H. , Mangini N.J. , Huang H. , Canfield V.A. , Cheng K.C. , Yang F. , Abe R. , Yamagishi S. , Shabanowitz J. , Hearing V.J. , Wu C. , Appella E. , Hunt D.F.
      J. Proteome Res.5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]
      tissue: Melanoma.
    12. 12.
      "Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR."
      Royer-Zemmour B. , Ponsole-Lenfant M. , Gara H. , Roll P. , Leveque C. , Massacrier A. , Ferracci G. , Cillario J. , Robaglia-Schlupp A. , Vincentelli R. , Cau P. , Szepetowski P.
      Hum. Mol. Genet.17:3617-3630(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SRPX2
    13. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    14. 14.
      "The refined 2.15 A X-ray crystal structure of human liver cathepsin B: the structural basis for its specificity."
      Musil D. , Zucic D. , Turk D. , Engh R.A. , Mayr I. , Huber R. , Popovic T. , Turk V. , Towatari T. , Katunuma N. , Bode W.
      EMBO J.10:2321-2330(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS)
    15. 15.
      "Crystal structures of human procathepsin B at 3.2- and 3.3-A resolution reveal an interaction motif between a papain-like cysteine protease and its propeptide."
      Turk D. , Podobnik M. , Kuhelj R. , Dolinar M. , Turk V.
      FEBS Lett.384:211-214(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS)
    16. 16.
      "Crystal structure of the wild-type human procathepsin B at 2.5-A resolution reveals the native active site of a papain-like cysteine protease zymogen."
      Podobnik M. , Kuhelj R. , Turk V. , Turk D.
      J. Mol. Biol.271:774-788(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
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