Ccl3 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comCcl3 ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Ccl3(Gene name) > Rat

Ccl3 (Gene name),

C-C motif chemokine 3 (Protein name ), CCL3_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Ccl3(Mip1a;Scya3);

Protein name:

C-C motif chemokine 3;

Alternative:

Small-inducible cytokine A3;Macrophage inflammatory protein 1-alpha(MIP-1-alpha);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

Sequence:

50:

92:

FETSSLCSQP | GVIFLTKRNR | QICADPKETW | VQEYITELEL | NA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00048

String:

P50229

KEGG:

rno:25542

UniGene:

Rn.10139

SMR:

P50229

Uniprot:

P50229

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ELISA Kit for Rat C-C motif chemokine 3

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E0092h

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ELISA Kit for Rat C-C motif chemokine 3

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E0092r

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ELISA Kit for Rat C-C motif chemokine 3

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E0092m

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ELISA Kit for Rat C-C motif chemokine 3

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E0092b

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96T

ELISA Kit for Rat C-C motif chemokine 3

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E0092d

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96T

CLIA Kit for Rat C-C motif chemokine 3

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U0092m

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96T

CLIA Kit for Rat C-C motif chemokine 3

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U0092b

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96T

CLIA Kit for Rat C-C motif chemokine 3

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U0092d

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96T

CLIA Kit for Rat C-C motif chemokine 3

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U0092r

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Looking for manual.

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96T

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CLIA Kit for Rat C-C motif chemokine 3

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U0092h

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Polyclonal Antibody for Rat C-C motif chemokine 3

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P0092Rb-m

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40ug/0.2ml

Polyclonal Antibody for Rat C-C motif chemokine 3

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P0092Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat C-C motif chemokine 3

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P0092Rb-h

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40ug/0.2ml

Polyclonal Antibody for Rat C-C motif chemokine 3

Monoclonal Antibody for Rat C-C motif chemokine 3

Protein for Rat C-C motif chemokine 3

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 7.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-108
Plasma	99	94-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-99%	91-102%	95-103%	96-107%
EDTA plasma(n=5)	91-97%	95-101%	94-102%	93-106%
heparin plasma(n=5)	93-104%	94-107%	101-108%	95-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
Shi M.M. , Godleski J.J. , Paulauskis J.D.
Biochem. Biophys. Res. Commun.211:289-295(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Lung.
2.

"Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
Shanley T.P. , Schmal H. , Friedl H.P. , Jones M.L. , Ward P.A.
J. Immunol.154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Long Evans.
tissue: Lung.
3.

"Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
Saad Y. , Garrett M.R. , Manickavasagam E. , Yerga-Woolwine S. , Farms P. , Radecki T. , Joe B.
Genomics89:343-353(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Dahl salt-sensitive.
strain: Lewis.
4.

"Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
Nakagawa H. , Shiota S. , Takano K. , Shibata F. , Kato H.
Biochem. Biophys. Res. Commun.220:945-948(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-57
strain: Wistar.

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