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Index > Protein center > Hspd1(Gene name) > Rat
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  • Hspd1 (Gene name),
  • 60 kDa heat shock protein, mitochondrial (Protein name ),  CH60_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Hspd1(Hsp60);
    Protein name:
    60 kDa heat shock protein, mitochondrial;
    Alternative:
    Mitochondrial matrix protein P1;Heat shock protein 60(HSP-60;Hsp60);HSP-65;60 kDa chaperonin;Chaperonin 60(CPN60);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Interacts with HRAS.
    Function:
    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
    Subcellular Location:
    Mitochondrion matrix
    Protein Attributes:
    Sequence length:
    573
    Sequence:
    50:
    MLRLPTVLRQ | MRPVSRALAP | HLTRAYAKDV | KFGADARALM | LQGVDLLADA | 
    100:
    VAVTMGPKGR | TVIIEQSWGS | PKVTKDGVTV | AKSIDLKDKY | KNIGAKLVQD | 
    150:
    VANNTNEEAG | DGTTTATVLA | RSIAKEGFEK | ISKGANPVEI | RRGVMLAVDA | 
    200:
    VIAELKKQSK | PVTTPEEIAQ | VATISANGDK | DIGNIISDAM | KKVGRKGVIT | 
    250:
    VKDGKTLNDE | LEIIEGMKFD | RGYISPYFIN | TSKGQKCEFQ | DAYVLLSEKK | 
    300:
    ISSVQSIVPA | LEIANAHRKP | LVIIAEDVDG | EALSTLVLNR | LKVGLQVVAV | 
    350:
    KAPGFGDNRK | NQLKDMAIAT | GGAVFGEEGL | NLNLEDVQAH | DLGKVGEVIV | 
    400:
    TKDDAMLLKG | KGDKAHIEKR | IQEITEQLDI | TTSEYEKEKL | NERLAKLSDG | 
    450:
    VAVLKVGGTS | DVEVNEKKDR | VTDALNATRA | AVEEGIVLGG | GCALLRCIPA | 
    500:
    LDSLKPANED | QKIGIEIIKR | ALKIPAMTIA | KNAGVEGSLI | VEKILQSSSE | 
    550:
    VGYDAMLGDF | VNMVEKGIID | PTKVVRTALL | DAAGVASLLT | TAEAVVTEIP | 
    573:
    KEEKDPGMGA | MGGMGGGMGG | GMF
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    UniGene:
    String:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
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    E0822h
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    96T
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    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
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    E0822m
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    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
    MSDS:
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    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    E0822r
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    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    E0822c
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    ELISA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    E0822b
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    Packing:
    96T
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    U0822c
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    U0822r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    U0822b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    MSDS:
    Please sign in first.
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    U0822m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    U0822h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    P0822Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    P0822Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Cat.:
    P0822Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Monoclonal Antibody for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial
    Protein for Rat 60 kDa heat shock protein, mitochondrial

    R&D Technical Data
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    s
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "cDNA and deduced amino acid sequence of rat liver prehsp60 (chaperonin-60)."
      Peralta D. , Hartman D.J. , McIntosh A.M. , Hoogenraad N.J. , Hoej P.B.
      Nucleic Acids Res.18:7162-7162(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Wistar.
      tissue: Liver.
    2. 2.
      Ryan M.T. , Herd S.M. , Sberna G. , Samuel M.M. , Hoogenraad N.J. , Hoej P.B.
      Submitted (1997-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: Sprague-Dawley.
      strain: Wistar.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Heart.
    4. 4.
      "Nucleotide sequence of rat hsp60 (chaperonin, GroEL homolog) cDNA."
      Venner T.J. , Gupta R.S.
      Nucleic Acids Res.18:5309-5309(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-573
      tissue: Kidney.
    5. 5.
      Lubec G. , Afjehi-Sadat L. , Diao W. , Kang S.U.
      Submitted (2007-07) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 38-58; 61-72; 143-157; 222-233; 237-309; 345-352; 371-387; 397-405; 421-446; 463-469 AND 482-493;IDENTIFICATION BY MASS SPECTROMETRY
      strain: Sprague-Dawley.
      tissue: Brain.
      tissue: Hippocampus.
      tissue: Spinal cord.
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