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Index > Protein center > C4B(Gene name) > Human
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  • C4B (Gene name),
  • Complement C4-B (Protein name ),  CO4B_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    C4B(CO4;CPAMD3);
    Protein name:
    Complement C4-B;
    Alternative:
    C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3;Basic complement C4;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Circulates in blood as a disulfide-linked trimer of an alpha, beta and gamma chain.
    Function:
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    1744
    Sequence:
    50:
    MRLLWGLIWA | SSFFTLSLQK | PRLLLFSPSV | VHLGVPLSVG | VQLQDVPRGQ | 
    100:
    VVKGSVFLRN | PSRNNVPCSP | KVDFTLSSER | DFALLSLQVP | LKDAKSCGLH | 
    150:
    QLLRGPEVQL | VAHSPWLKDS | LSRTTNIQGI | NLLFSSRRGH | LFLQTDQPIY | 
    200:
    NPGQRVRYRV | FALDQKMRPS | TDTITVMVEN | SHGLRVRKKE | VYMPSSIFQD | 
    250:
    DFVIPDISEP | GTWKISARFS | DGLESNSSTQ | FEVKKYVLPN | FEVKITPGKP | 
    300:
    YILTVPGHLD | EMQLDIQARY | IYGKPVQGVA | YVRFGLLDED | GKKTFFRGLE | 
    350:
    SQTKLVNGQS | HISLSKAEFQ | DALEKLNMGI | TDLQGLRLYV | AAAIIESPGG | 
    400:
    EMEEAELTSW | YFVSSPFSLD | LSKTKRHLVP | GAPFLLQALV | REMSGSPASG | 
    450:
    IPVKVSATVS | SPGSVPEVQD | IQQNTDGSGQ | VSIPIIIPQT | ISELQLSVSA | 
    500:
    GSPHPAIARL | TVAAPPSGGP | GFLSIERPDS | RPPRVGDTLN | LNLRAVGSGA | 
    550:
    TFSHYYYMIL | SRGQIVFMNR | EPKRTLTSVS | VFVDHHLAPS | FYFVAFYYHG | 
    600:
    DHPVANSLRV | DVQAGACEGK | LELSVDGAKQ | YRNGESVKLH | LETDSLALVA | 
    650:
    LGALDTALYA | AGSKSHKPLN | MGKVFEAMNS | YDLGCGPGGG | DSALQVFQAA | 
    700:
    GLAFSDGDQW | TLSRKRLSCP | KEKTTRKKRN | VNFQKAINEK | LGQYASPTAK | 
    750:
    RCCQDGVTRL | PMMRSCEQRA | ARVQQPDCRE | PFLSCCQFAE | SLRKKSRDKG | 
    800:
    QAGLQRALEI | LQEEDLIDED | DIPVRSFFPE | NWLWRVETVD | RFQILTLWLP | 
    850:
    DSLTTWEIHG | LSLSKTKGLC | VATPVQLRVF | REFHLHLRLP | MSVRRFEQLE | 
    900:
    LRPVLYNYLD | KNLTVSVHVS | PVEGLCLAGG | GGLAQQVLVP | AGSARPVAFS | 
    950:
    VVPTAAAAVS | LKVVARGSFE | FPVGDAVSKV | LQIEKEGAIH | REELVYELNP | 
    1000:
    LDHRGRTLEI | PGNSDPNMIP | DGDFNSYVRV | TASDPLDTLG | SEGALSPGGV | 
    1050:
    ASLLRLPRGC | GEQTMIYLAP | TLAASRYLDK | TEQWSTLPPE | TKDHAVDLIQ | 
    1100:
    KGYMRIQQFR | KADGSYAAWL | SRDSSTWLTA | FVLKVLSLAQ | EQVGGSPEKL | 
    1150:
    QETSNWLLSQ | QQADGSFQDL | SPVIHRSMQG | GLVGNDETVA | LTAFVTIALH | 
    1200:
    HGLAVFQDEG | AEPLKQRVEA | SISKANSFLG | EKASAGLLGA | HAAAITAYAL | 
    1250:
    SLTKAPVDLL | GVAHNNLMAM | AQETGDNLYW | GSVTGSQSNA | VSPTPAPRNP | 
    1300:
    SDPMPQAPAL | WIETTAYALL | HLLLHEGKAE | MADQASAWLT | RQGSFQGGFR | 
    1350:
    STQDTVIALD | ALSAYWIASH | TTEERGLNVT | LSSTGRNGFK | SHALQLNNRQ | 
    1400:
    IRGLEEELQF | SLGSKINVKV | GGNSKGTLKV | LRTYNVLDMK | NTTCQDLQIE | 
    1450:
    VTVKGHVEYT | MEANEDYEDY | EYDELPAKDD | PDAPLQPVTP | LQLFEGRRNR | 
    1500:
    RRREAPKVVE | EQESRVHYTV | CIWRNGKVGL | SGMAIADVTL | LSGFHALRAD | 
    1550:
    LEKLTSLSDR | YVSHFETEGP | HVLLYFDSVP | TSRECVGFEA | VQEVPVGLVQ | 
    1600:
    PASATLYDYY | NPERRCSVFY | GAPSKSRLLA | TLCSAEVCQC | AEGKCPRQRR | 
    1650:
    ALERGLQDED | GYRMKFACYY | PRVEYGFQVK | VLREDSRAAF | RLFETKITQV | 
    1700:
    LHFTKDVKAA | ANQMRNFLVR | ASCRLRLEPG | KEYLIMGLDG | ATYDLEGHPQ | 
    1744:
    YLLDSNSWIE | EMPSERLCRS | TRQRAACAQL | NDFLQEYGTQ | GCQV
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    String:
    SMR:
    MIM:
    KEGG:
    UniGene:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Complement C4-B
    Cat.:
    E11254m
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Complement C4-B
    Cat.:
    E11254h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Complement C4-B
    Cat.:
    U11254m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Complement C4-B
    Cat.:
    U11254h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Complement C4-B
    Cat.:
    P11254Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Complement C4-B
    Monoclonal Antibody for Human Complement C4-B
    Monoclonal Antibody for Human Complement C4-B
    Protein for Human Complement C4-B
    Protein for Human Complement C4-B

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.9%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of CO4B into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    94-104

    Plasma

    99

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CO4B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-110%

    94-107%

    95-105%

    93-109%

    EDTA plasma(n=5)

    94-105%

    96-102%

    92-104%

    90-108%

    heparin plasma(n=5)

    93-107%

     

    91-106%

    93-106%

    94-103%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Complete sequence of the complement C4 gene from the HLA-A1, B8, C4AQ0, C4B1, DR3 haplotype."
      Ulgiati D. , Townend D.C. , Christiansen F.T. , Dawkins R.L. , Abraham L.J.
      Immunogenetics43:250-252(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT PHE-1317
      tissue: Blood.
    2. 2.
      "Sequence determination of 300 kilobases of the human class III MHC locus."
      Rowen L. , Dankers C. , Baskin D. , Faust J. , Loretz C. , Ahearn M.E. , Banta A. , Swartzell S. , Smith T.M. , Spies T. , Hood L.
      Submitted (1999-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS TYR-347 AND ALA-907
    3. 3.
      "Molecular genetics of complement C4: implications for MHC evolution and disease susceptibility gene mapping."
      Sayer D. , Puschendorf M. , Wetherall J.
      Submitted (2003-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ALA-907 AND ASP-1073
    4. 4.
      "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J. , Palmer S.A. , Sims S.K. , Edwards C.A. , Ashurst J.L. , Wilming L. , Jones M.C. , Horton R. , Hunt S.E. , Scott C.E. , Gilbert J.G.R. , Clamp M.E. , Bethel G. , Milne S. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Andrews T.D. , more...
      Nature425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANTS TYR-347 AND ALA-907
    5. 5.
      "Complete primary structure of human C4a anaphylatoxin."
      Moon K.E. , Gorski J.P. , Hugli T.E.
      J. Biol. Chem.256:8685-8692(1981) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 680-756
    6. 6.
      "Importance of the alpha 3-fragment of complement C4 for the binding with C4b-binding protein."
      Hessing M. , van 't Veer C. , Hackeng T.M. , Bouma B.N. , Iwanaga S.
      FEBS Lett.271:131-136(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 757-771 AND 980-990
    7. 7.
      "The structural basis of the multiple forms of human complement component C4."
      Belt K.T. , Carroll M.C. , Porter R.R.
      Cell36:907-914(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 956-1336;VARIANT ASP-1073
      tissue: Liver.
    8. 8.
      "Amino acid sequence around the thiol and reactive acyl groups of human complement component C4."
      Campbell R.D. , Gagnon J. , Porter R.R.
      Biochem. J.199:359-370(1981) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 957-1044
    9. 9.
      "The chemical structure of the C4d fragment of the human complement component C4."
      Chakravarti D.N. , Campbell R.D. , Porter R.R.
      Mol. Immunol.24:1187-1197(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 957-1336
    10. 10.
      "Sequence determination of the thiolester site of the fourth component of human complement."
      Harrison R.A. , Thomas M.L. , Tack B.F.
      Proc. Natl. Acad. Sci. U.S.A.78:7388-7392(1981) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 990-1037
    11. 11.
      "C4d DNA sequences of two infrequent human allotypes (C4A13 and C4B12) and the presence of signal sequences enhancing recombination."
      Martinez-Quiles N. , Paz-Artal E. , Moreno-Pelayo M.A. , Longas J. , Ferre-Lopez S. , Rosal M. , Arnaiz-Villena A.
      J. Immunol.161:3438-3443(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1055-1225 (ALLOTYPE C4B12)
    12. 12.
      "C4d DNA sequence of complement C4B93 and recombination mechanisms for its generation."
      Lopez-Goyanes A. , Moreno M.A. , Ferre S. , Paz-Artal E.
      Tissue Antigens63:260-262(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1055-1225;VARIANTS ASN-1176; VAL-1207 AND LEU-1210
    13. 13.
      "Amino acid sequence of a polymorphic segment from fragment C4d of human complement component C4."
      Chakravarti D.N. , Campbell R.D. , Gagnon J.
      FEBS Lett.154:387-390(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1199-1304
    14. 14.
      "Identification of the site of sulfation of the fourth component of human complement."
      Hortin G. , Sims H. , Strauss A.W.
      J. Biol. Chem.261:1786-1793(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1405-1431;SULFATION AT TYR-1417; TYR-1420 AND TYR-1422
    15. 15.
      "Complete C4B deficiency in black Americans with systemic lupus erythematosus."
      Wilson W.A. , Perez M.C.
      J. Rheumatol.15:1855-1858(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN C4BD AND SLE
    16. 16.
      "Substitution of a single amino acid (aspartic acid for histidine) converts the functional activity of human complement C4B to C4A."
      Carroll M.C. , Fathallah D.M. , Bergamaschini L. , Alicot E.M. , Isenman D.E.
      Proc. Natl. Acad. Sci. U.S.A.87:6868-6872(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INVOLVEMENT OF HIS-1125 IN IMMUNOGLOBULIN-BINDING AND HEMOLYSIS;MUTAGENESIS OF LEU-1120; SER-1121; ILE-1124 AND HIS-1125
    17. 17.
      "The reaction mechanism of the internal thioester in the human complement component C4."
      Dodds A.W. , Ren X.D. , Willis A.C. , Law S.K.
      Nature379:177-179(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    18. 18.
      "Deficiency of human complement protein C4 due to identical frameshift mutations in the C4A and C4B genes."
      Lokki M.L. , Circolo A. , Ahokas P. , Rupert K.L. , Yu C.Y. , Colten H.R.
      J. Immunol.162:3687-3693(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SLE
    19. 19.
      "Genetic, structural and functional diversities of human complement components C4A and C4B and their mouse homologues, Slp and C4."
      Blanchong C.A. , Chung E.K. , Rupert K.L. , Yang Y. , Yang Z. , Zhou B. , Moulds J.M. , Yu C.Y.
      Int. Immunopharmacol.1:365-392(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW;DESCRIPTION OF ALLOTYPES;TISSUE SPECIFICITY
    20. 20.
      "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
      Zhang H. , Li X.-J. , Martin D.B. , Aebersold R.
      Nat. Biotechnol.21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-226
    21. 21.
      "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
      Bunkenborg J. , Pilch B.J. , Podtelejnikov A.V. , Wisniewski J.R.
      Proteomics4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1391
      tissue: Plasma.
    22. 22.
      "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
      J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1328
      tissue: Saliva.
    23. 23.
      "Structural basis of the polymorphism of human complement components C4A and C4B: gene size, reactivity and antigenicity."
      Yu C.Y. , Belt K.T. , Giles C.M. , Campbell R.D. , Porter R.R.
      EMBO J.5:2873-2881(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURAL BASIS OF POLYMORPHISM
    24. 24.
      "Gene copy-number variation and associated polymorphisms of complement component C4 in human systemic lupus erythematosus (SLE): low copy number is a risk factor for and high copy number is a protective factor against SLE susceptibility in European Americans."
      Yang Y. , Chung E.K. , Wu Y.L. , Savelli S.L. , Nagaraja H.N. , Zhou B. , Hebert M. , Jones K.N. , Shu Y. , Kitzmiller K. , Blanchong C.A. , McBride K.L. , Higgins G.C. , Rennebohm R.M. , Rice R.R. , Hackshaw K.V. , Roubey R.A. , Grossman J.M. , more...
      Am. J. Hum. Genet.80:1037-1054(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SLE
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