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Index > Protein center > CSF2(Gene name) > Human
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  • CSF2 (Gene name),
  • Granulocyte-macrophage colony-stimulating factor (Protein name ),  CSF2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    CSF2(GMCSF);
    Protein name:
    Granulocyte-macrophage colony-stimulating factor(GM-CSF);
    Alternative:
    Molgramostin;Colony-stimulating factor(CSF);Sargramostim;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    144
    Sequence:
    50:
    MWLQSLLLLG | TVACSISAPA | RSPSPSTQPW | EHVNAIQEAR | RLLNLSRDTA | 
    100:
    AEMNETVEVI | SEMFDLQEPT | CLQTRLELYK | QGLRGSLTKL | KGPLTMMASH | 
    144:
    YKQHCPPTPE | TSCATQIITF | ESFKENLKDF | LLVIPFDCWE | PVQE
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    Pfam:
    UniGene:
    SMR:
    MIM:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045m
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human GM-CSF
    Cat.:
    E0045d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human GM-CSF
    Cat.:
    U0045m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Human GM-CSF
    Polyclonal Antibody for Human GM-CSF
    Cat.:
    P0045Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human GM-CSF
    Cat.:
    P0045Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human GM-CSF
    Polyclonal Antibody for Human GM-CSF
    Cat.:
    P0045Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Monoclonal Antibody for Human GM-CSF
    Protein for Human GM-CSF
    Protein for Human GM-CSF
    Protein for Human GM-CSF
    Protein for Human GM-CSF
    Protein for Human GM-CSF
    Protein for Human GM-CSF

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.1%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.5%

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    Recovery
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    Recovery was determined by spiking various levels of CSF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    95-103

    Plasma

    100

    98-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-102%

    96-104%

    97-101%

    98-102%

    EDTA plasma(n=5)

    95-106%

    97-103%

    92-102%

    98-100%

    heparin plasma(n=5)

    93-104%

     

    102-108%

    98-105%

    96-108%

     

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    References
    1. 1.
      "Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells."
      Lee F. , Yokota T. , Otsuka T. , Gemmell L. , Larson N. , Luh J. , Arai K. , Rennick D.
      Proc. Natl. Acad. Sci. U.S.A.82:4360-4364(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Genomic cloning, characterization, and multilineage growth-promoting activity of human granulocyte-macrophage colony-stimulating factor."
      Kaushansky K. , O'Hara P.J. , Berkner K. , Segal G.M. , Hagen F.S. , Adamson J.W.
      Proc. Natl. Acad. Sci. U.S.A.83:3101-3105(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor."
      Cantrell M.A. , Anderson D. , Cerretti D.P. , Price V. , McKereghan K. , Tushinski R.J. , Mochizuki D.Y. , Larsen A. , Grabstein S. , Gillis S. , Cosman D.
      Proc. Natl. Acad. Sci. U.S.A.82:6250-6254(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins."
      Wong G.G. , Witek J.S. , Temple P.A. , Wilkens K.M. , Leary A.C. , Luxenberg D.P. , Jones S.S. , Brown E.L. , Kay R.M. , Orr E.C. , Shoemaker C. , Golde D.W. , Kaufman R.J. , Hewick R.M. , Wang E.A. , Clark S.C.
      Science228:810-815(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    5. 5.
      "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes."
      Miyatake S. , Otsuka T. , Yokota T. , Lee F. , Arai K.
      EMBO J.4:2561-2568(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    6. 6.
      SeattleSNPs variation discovery resource
      Submitted (2001-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ILE-115 AND THR-117
    7. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT THR-117
    8. 9.
      Bhattacharya P. , Pandey G. , Mukherjee K.J.
      Submitted (2002-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-144;VARIANT THR-117
      tissue: Peripheral blood.
    9. 10.
      "Role of carbohydrate modification in the production and secretion of human granulocyte macrophage colony-stimulating factor in genetically engineered and normal mesenchymal cells."
      Kaushansky K. , Lopez J.A. , Brown C.B.
      Biochemistry31:1881-1886(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-22; SER-24; SER-26 AND THR-27
    10. 11.
      "Novel fold and putative receptor binding site of granulocyte-macrophage colony-stimulating factor."
      Diederichs K. , Boone T. , Karplus P.A.
      Science254:1779-1782(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)
    11. 12.
      "Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor."
      Walter M.R. , Cook W.J. , Ealick S.E. , Nagabhushan T.L. , Trotta P.P. , Bugg C.E.
      J. Mol. Biol.224:1075-1085(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS)
    12. 13.
      "The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation."
      Hansen G. , Hercus T.R. , McClure B.J. , Stomski F.C. , Dottore M. , Powell J. , Ramshaw H. , Woodcock J.M. , Xu Y. , Guthridge M. , McKinstry W.J. , Lopez A.F. , Parker M.W.
      Cell134:496-507(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 18-144 IN COMPLEX WITH CSF2RA AND CSF2RB;SUBUNIT;DISULFIDE BOND
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