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Index > Protein center > Csf2(Gene name) > Mouse
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  • Csf2 (Gene name),
  • Granulocyte-macrophage colony-stimulating factor (Protein name ),  CSF2_MOUSE from NCBI database.
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  • General Annotation
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    • Gene name:
    Csf2(Csfgm);
    Protein name:
    Granulocyte-macrophage colony-stimulating factor(GM-CSF);
    Alternative:
    Colony-stimulating factor(CSF);
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    141
    Sequence:
    50:
    MWLQNLLFLG | IVVYSLSAPT | RSPITVTRPW | KHVEAIKEAL | NLLDDMPVTL | 
    100:
    NEEVEVVSNE | FSFKKLTCVQ | TRLKIFEQGL | RGNFTKLKGA | LNMTASYYQT | 
    141:
    YCPPTPETDC | ETQVTTYADF | IDSLKTFLTD | IPFECKKPGQ | K
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    PF01109
    UniGene:
    Mm.4922
    SMR:
    P01587
    String:
    P01587
    KEGG:
    mmu:12981
    Uniprot:
    P01587
     
    FOR
    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045m
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    96T
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    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045h
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    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045r
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    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045b
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    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045p
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    ELISA Kit for Mouse GM-CSF
    Cat.:
    E0045d
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    Packing:
    96T
    CLIA Kit for Mouse GM-CSF
    Cat.:
    U0045p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse GM-CSF
    Cat.:
    U0045d
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    Packing:
    96T
    CLIA Kit for Mouse GM-CSF
    Cat.:
    U0045b
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    CLIA Kit for Mouse GM-CSF
    Cat.:
    U0045r
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    96T
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    CLIA Kit for Mouse GM-CSF
    Cat.:
    U0045h
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    CLIA Kit for Mouse GM-CSF
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    U0045m
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    Polyclonal Antibody for Mouse GM-CSF
    Polyclonal Antibody for Mouse GM-CSF
    Cat.:
    P0045Rb-r
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse GM-CSF
    Cat.:
    P0045Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse GM-CSF
    Polyclonal Antibody for Mouse GM-CSF
    Cat.:
    P0045Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Monoclonal Antibody for Mouse GM-CSF
    Protein for Mouse GM-CSF
    Protein for Mouse GM-CSF
    Protein for Mouse GM-CSF
    Protein for Mouse GM-CSF
    Protein for Mouse GM-CSF
    Protein for Mouse GM-CSF

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.1%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.5%

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    Recovery
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    Recovery was determined by spiking various levels of CSF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    95-103

    Plasma

    100

    98-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    92-102%

    96-104%

    97-101%

    98-102%

    EDTA plasma(n=5)

    95-106%

    97-103%

    92-102%

    98-100%

    heparin plasma(n=5)

    93-104%

     

    102-108%

    98-105%

    96-108%

     

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    References
    1. 1.
      "Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes."
      Miyatake S. , Otsuka T. , Yokota T. , Lee F. , Arai K.
      EMBO J.4:2561-2568(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters."
      Stanley E.R. , Metcalf D. , Sobieszczuk P. , Gough N.M. , Dunn A.R.
      EMBO J.4:2569-2573(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum."
      Delamarter J.F. , Mermod J.-J. , Liang C.M. , Eliason J.F. , Thatcher D.R.
      EMBO J.4:2575-2581(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Structure and expression of the mRNA for murine granulocyte-macrophage colony stimulating factor."
      Gough N.M. , Metcalf D. , Gough J. , Grail D. , Dunn A.R.
      EMBO J.4:645-653(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: BALB/c.
    5. 5.
      "Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor."
      Gough N.M. , Gough J. , Metcalf D. , Kelso A. , Grail D. , Nicola N.A. , Burgess A.W. , Dunn A.R.
      Nature309:763-767(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-141
      tissue: Lung.
    6. 6.
      "Cloning, sequence, and expression of a human granulocyte/macrophage colony-stimulating factor."
      Cantrell M.A. , Anderson D. , Cerretti D.P. , Price V. , McKereghan K. , Tushinski R.J. , Mochizuki D.Y. , Larsen A. , Grabstein S. , Gillis S. , Cosman D.
      Proc. Natl. Acad. Sci. U.S.A.82:6250-6254(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-141
    7. 7.
      "Purification and partial amino acid sequence of asialo murine granulocyte-macrophage colony stimulating factor."
      Sparrow L.G. , Metcalf D. , Hunkapiller M.W. , Hood L.E. , Burgess A.W.
      Proc. Natl. Acad. Sci. U.S.A.82:292-296(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-57
    8. 8.
      "Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli."
      Schrimser J.L. , Rose K. , Simona M.G. , Wingfield P.
      Biochem. J.247:195-199(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
    9. 9.
      "Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation."
      Altmann S.W. , Johnson G.D. , Prystowsky M.B.
      J. Biol. Chem.266:5333-5341(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS
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