DNAJC27 ELISA Kit for Human(Homo sapiens) | EIAab.comDNAJC27 ELISA Kit for Human(Homo sapiens) | EIAab.com

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DNAJC27 (Gene name),

DnaJ homolog subfamily C member 27 (Protein name ), DJC27_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

DNAJC27(RABJS;RBJ);

Protein name:

DnaJ homolog subfamily C member 27;

Alternative:

Rab and DnaJ domain-containing protein;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

N/A

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

273

Sequence:

50:

MEANMPKRKE | PGRSLRIKVI | SMGNAEVGKS | CIIKRYCEKR | FVSKYLATIG |

100:

IDYGVTKVHV | RDREIKVNIF | DMAGHPFFYE | VRNEFYKDTQ | GVILVYDVGQ |

150:

KDSFDALDAW | LAEMKQELGP | HGNMENIIFV | VCANKIDCTK | HRCVDESEGR |

200:

LWAESKGFLY | FETSAQTGEG | INEMFQTFYI | SIVDLCENGG | KRPTTNSSAS |

250:

FTKEQADAIR | RIRNSKDSWD | MLGVKPGASR | DEVNKAYRKL | AVLLHPDKCV |

273:

APGSEDAFKA | VVNARTALLK | NIK

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

MIM:

Pfam:

KEGG:

UniGene:

SMR:

STRING:

9606.ENSP00000264711

Uniprot:

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ELISA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

E3713h

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96T

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ELISA Kit for Human DnaJ homolog subfamily C member 27

MSDS:

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ELISA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

E3713m

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96T

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ELISA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

E3713r

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ELISA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

E3713b

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MSDS:

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Packing:

96T

CLIA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

U3713m

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MSDS:

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Packing:

96T

CLIA Kit for Human DnaJ homolog subfamily C member 27

MSDS:

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CLIA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

U3713h

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CLIA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

U3713b

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96T

CLIA Kit for Human DnaJ homolog subfamily C member 27

Cat.:

U3713r

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Polyclonal Antibody for Human DnaJ homolog subfamily C member 27

Polyclonal Antibody for Human DnaJ homolog subfamily C member 27

Polyclonal Antibody for Human DnaJ homolog subfamily C member 27

Cat.:

P3713Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human DnaJ homolog subfamily C member 27

Polyclonal Antibody for Human DnaJ homolog subfamily C member 27

Cat.:

P3713Rb-m

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Monoclonal Antibody for Human DnaJ homolog subfamily C member 27

Monoclonal Antibody for Human DnaJ homolog subfamily C member 27

Monoclonal Antibody for Human DnaJ homolog subfamily C member 27

Monoclonal Antibody for Human DnaJ homolog subfamily C member 27

Monoclonal Antibody for Human DnaJ homolog subfamily C member 27

Protein for Human DnaJ homolog subfamily C member 27

Protein for Human DnaJ homolog subfamily C member 27

Protein for Human DnaJ homolog subfamily C member 27

Protein for Human DnaJ homolog subfamily C member 27

Protein for Human DnaJ homolog subfamily C member 27

R&D Technical Data

12% SDS-PAGE Analysis

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.4%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

Recovery was determined by spiking various levels of DJC27 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	85-96
Plasma	94	87-102

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DJC27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-100%	90-96%	85-98%	89-95%
EDTA plasma(n=5)	88-101%	87-102%	90-97%	85-101%
heparin plasma(n=5)	90-99%	86-101%	91-98%	84-103%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Identification of a unique Rab GTPase containing a J domain that interacts with Hsc70."
Chen T. , Zhang W. , Li N. , Wan T. , Zhang M. , Chen G. , Zhang Y. , Cao X.
Submitted (2002-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3)
2.

"The full-ORF clone resource of the German cDNA consortium."
Bechtel S. , Rosenfelder H. , Duda A. , Schmidt C.P. , Ernst U. , Wellenreuther R. , Mehrle A. , Schuster C. , Bahr A. , Bloecker H. , Heubner D. , Hoerlein A. , Michel G. , Wedler H. , Koehrer K. , Ottenwaelder B. , Poustka A. , Wiemann S. , more...
BMC Genomics8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Testis.
3.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Brain.
5.

"RJLs: a new family of Ras-related GTP-binding proteins."
Nepomuceno-Silva J.L. , de Melo L.D. , Mendonca S.M. , Paixao J.C. , Lopes U.G.
Gene327:221-232(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION;POSSIBLE LACK OF GTPASE ACTIVITY
6.

"Small GTPase RBJ mediates nuclear entrapment of MEK1/MEK2 in tumor progression."
Chen T. , Yang M. , Yu Z. , Tang S. , Wang C. , Zhu X. , Guo J. , Li N. , Zhang W. , Hou J. , Liu H. , Han C. , Liu Q. , Gu Y. , Qian C. , Wan T. , Cui L. , Zhu M. , more...
Cancer Cell25:682-696(2014) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
7.

"Solution structure of the dnaJ-like domain from human Ras-associated protein RAP1."
RIKEN structural genomics initiative (RSGI)
Submitted (2007-10) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 197-273

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