DKK4 ELISA Kit for Human(Homo sapiens) | EIAab.comDKK4 ELISA Kit for Human(Homo sapiens)

Search Protein-oriented Product center.

For antibodies, ELISA/CLIA, proteins... Product center

Tips

Index > Protein center > DKK4(Gene name) > Human

DKK4 (Gene name),

Dickkopf-related protein 4 (Protein name ), DKK4_HUMAN from NCBI database.

top

General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Feedback

Gene name:

DKK4;

Protein name:

Dickkopf-related protein 4(Dickkopf-4;Dkk-4;hDkk-4);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with LRP5 and LRP6.

Function:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

224

Sequence:

50:

100:

150:

200:

224:

LCRSQLTSNR | QHARLRVCQK | IEKL

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

Please Sign in.

Related Databases

Pfam:

PF04706

String:

Q9UBT3

MIM:

605417

STRING:

9606.ENSP00000220812

SMR:

Q9UBT3

KEGG:

hsa:27121

UniGene:

Hs.159311

Uniprot:

Q9UBT3

FOR

ELISA Kit for Human Dickkopf-4

Cat.:

E5890h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

ELISA Kit for Human Dickkopf-4

Cat.:

E5890m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Dickkopf-4

Cat.:

U5890h

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Dickkopf-4

Cat.:

U5890m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

Polyclonal Antibody for Human Dickkopf-4

Cat.:

P5890Rb-h

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Human Dickkopf-4

Cat.:

P5890Rb-m

Price:

Please sign in first.

Packing:

40ug/0.2ml

Monoclonal Antibody for Human Dickkopf-4

Protein for Human Dickkopf-4

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Functional and structural diversity of the human Dickkopf gene family."
Krupnik V.E. , Sharp J.D. , Jiang C. , Robison K. , Chickering T.W. , Amaravadi L. , Brown D.E. , Guyot D. , Mays G. , Leiby K. , Chang B. , Duong T. , Goodearl A.D.J. , Gearing D.P. , Sokol S.Y. , McCarthy S.A.
Gene238:301-313(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 19-28 AND 134-144;PROTEOLYTIC PROCESSING
2.

"Human Dickkopf as well as DAN family members, Cerberus and Gremlin, are preferentially expressed in the epithelial malignant cell lines."
Tate G. , Mitsuya T.
J. Biochem. Mol. Biol. Biophys.3:239-242(1999)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z. , Henzel W.J.
Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-33
5.

"Function and biological roles of the Dickkopf family of Wnt modulators."
Niehrs C.
Oncogene25:7469-7481(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW OF THE DKK FAMILY

Product Feedback Wall

* Email:
*Comments: