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Index > Protein center > DRD2(Gene name) > Human
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  • DRD2 (Gene name),
  • D(2) dopamine receptor (Protein name ),  DRD2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    DRD2;
    Protein name:
    D(2) dopamine receptor;
    Alternative:
    Dopamine D2 receptor;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Forms homo- and heterooligomers with DRD4. The interaction with DRD4 may modulate agonist-induced downstream signaling. Interacts with GPRASP1, PPP1R9B and CLIC6 (By similarity). Interacts with CADPS and CADPS2. Interacts with ARRB2 (By similarity). Interacts with GNAI1.
    Function:
    Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.
    Subcellular Location:
    Cell membrane Multi-pass membrane protein
    Protein Attributes:
    Sequence length:
    443
    Sequence:
    50:
    MDPLNLSWYD | DDLERQNWSR | PFNGSDGKAD | RPHYNYYATL | LTLLIAVIVF | 
    100:
    GNVLVCMAVS | REKALQTTTN | YLIVSLAVAD | LLVATLVMPW | VVYLEVVGEW | 
    150:
    KFSRIHCDIF | VTLDVMMCTA | SILNLCAISI | DRYTAVAMPM | LYNTRYSSKR | 
    200:
    RVTVMISIVW | VLSFTISCPL | LFGLNNADQN | ECIIANPAFV | VYSSIVSFYV | 
    250:
    PFIVTLLVYI | KIYIVLRRRR | KRVNTKRSSR | AFRAHLRAPL | KGNCTHPEDM | 
    300:
    KLCTVIMKSN | GSFPVNRRRV | EAARRAQELE | MEMLSSTSPP | ERTRYSPIPP | 
    350:
    SHHQLTLPDP | SHHGLHSTPD | SPAKPEKNGH | AKDHPKIAKI | FEIQTMPNGK | 
    400:
    TRTSLKTMSR | RKLSQQKEKK | ATQMLAIVLG | VFIICWLPFF | ITHILNIHCD | 
    443:
    CNIPPVLYSA | FTWLGYVNSA | VNPIIYTTFN | IEFRKAFLKI | LHC
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    KEGG:
    MIM:
    Pfam:
    UniGene:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human D(2) dopamine receptor
    Cat.:
    E0673m
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human D(2) dopamine receptor
    Cat.:
    E0673r
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    Packing:
    96T
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    ELISA Kit for Human D(2) dopamine receptor
    Cat.:
    E0673h
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    96T
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    ELISA Kit for Human D(2) dopamine receptor
    Cat.:
    E0673b
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human D(2) dopamine receptor
    Cat.:
    E0673d
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human D(2) dopamine receptor
    Cat.:
    U0673m
    Price:
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human D(2) dopamine receptor
    Cat.:
    U0673h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human D(2) dopamine receptor
    Cat.:
    U0673r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human D(2) dopamine receptor
    Cat.:
    U0673b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human D(2) dopamine receptor
    Cat.:
    U0673d
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human D(2) dopamine receptor
    Polyclonal Antibody for Human D(2) dopamine receptor
    Cat.:
    P0673Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human D(2) dopamine receptor
    Polyclonal Antibody for Human D(2) dopamine receptor
    Cat.:
    P0673Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human D(2) dopamine receptor
    Cat.:
    P0673Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human D(2) dopamine receptor
    Monoclonal Antibody for Human D(2) dopamine receptor
    Monoclonal Antibody for Human D(2) dopamine receptor
    Monoclonal Antibody for Human D(2) dopamine receptor
    Monoclonal Antibody for Human D(2) dopamine receptor
    Protein for Human D(2) dopamine receptor
    Protein for Human D(2) dopamine receptor
    Protein for Human D(2) dopamine receptor
    Protein for Human D(2) dopamine receptor
    Protein for Human D(2) dopamine receptor

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.8%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of DRD2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    94-107

    Plasma

    100

    92-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DRD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    94-108%

    96-107%

    93-110%

    97-104%

    EDTA plasma(n=5)

    92-108%

    91-110%

    92-106%

    94-108%

    heparin plasma(n=5)

    95-106%

     

    93-107%

    95-105%

    91-109%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "The major dopamine D2 receptor: molecular analysis of the human D2A subtype."
      Selbie L.A. , Hayes G. , Shine J.
      DNA8:683-689(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    2. 2.
      "The dopamine D2 receptor: two molecular forms generated by alternative splicing."
      Dal-Toso R. , Sommer B. , Ewert M. , Herb A. , Pritchett D.B. , Bach A. , Shivers B.D. , Seeburg P.H.
      EMBO J.8:4025-4034(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2)
    3. 3.
      "Human retina D2 receptor cDNAs have multiple polyadenylation sites and differ from a pituitary clone at the 5' non-coding region."
      Robakis N.K. , Mohamadi M. , Fu D.Y. , Sambamurti K. , Refolo L.M.
      Nucleic Acids Res.18:1299-1299(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Retina.
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    5. 5.
      "Molecular cloning and expression of a dopamine D2 receptor from human retina."
      Stormann T.M. , Gdula D.C. , Weiner D.M. , Brann M.R.
      Mol. Pharmacol.37:1-6(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Retina.
    6. 6.
      "DNA homology screening: isolation and characterization of the human D2A dopamine receptor subtype."
      Selbie L.A. , Hayes G. , Shine J.
      Adv. Second Messenger Phosphoprotein Res.24:9-14(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1)
    7. 7.
      "Structure and expression of human and rat D2 dopamine receptor genes."
      Araki K. , Kuwano R. , Morii K. , Hayashi S. , Minoshima S. , Shimizu N. , Katagiri T. , Usui H. , Kumanishi T. , Takahashi Y.
      Neurochem. Int.21:91-98(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    8. 8.
      "D2 dopamine receptors in the human retina: cloning of cDNA and localization of mRNA."
      Dearry A. , Falardeau P. , Shores C. , Caron M.G.
      Cell. Mol. Neurobiol.11:437-453(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Retina.
    9. 9.
      "Schizophrenia: normal sequence in the dopamine D2 receptor region that couples to G-proteins. DNA polymorphisms in D2."
      Seeman P. , Ohara K. , Ulpian C. , Seeman M.V. , Jellinger K. , Tol H.H. , Niznik H.B.
      Neuropsychopharmacology8:137-142(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
      tissue: Brain.
    10. 10.
      "New dopamine receptor, D2(Longer), with unique TG splice site, in human brain."
      Seeman P. , Nam D. , Ulpian C. , Liu I.S.C. , Tallerico T.
      Brain Res. Mol. Brain Res.76:132-141(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3)
      tissue: Corpus striatum.
    11. 11.
      "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
      Suwa M. , Sato T. , Okouchi I. , Arita M. , Futami K. , Matsumoto S. , Tsutsumi S. , Aburatani H. , Asai K. , Akiyama Y.
      Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3)
    12. 12.
      Kidd K.K.
      Submitted (1998-02) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1)
    13. 13.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Lung.
    14. 14.
      "Regulation of dense core vesicle release from PC12 cells by interaction between the D2 dopamine receptor and calcium-dependent activator protein for secretion (CAPS)."
      Binda A.V. , Kabbani N. , Levenson R.
      Biochem. Pharmacol.69:1451-1461(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CADPS AND CADPS2
    15. 15.
      "Role of a Galphai2 protein splice variant in the formation of an intracellular dopamine D2 receptor pool."
      Lopez-Aranda M.F. , Acevedo M.J. , Gutierrez A. , Koulen P. , Khan Z.U.
      J. Cell Sci.120:2171-2178(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GNAI2
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: HOMOOLIGOMERIZATION;INTERACTION WITH DRD4
    17. 17.
      "Functional crosstalk and heteromerization of serotonin 5-HT2A and dopamine D2 receptors."
      Albizu L. , Holloway T. , Gonzalez-Maeso J. , Sealfon S.C.
      Neuropharmacology61:770-777(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HTR2A;FUNCTION;SUBCELLULAR LOCATION
    18. 18.
      "Structural basis for nucleotide exchange on G alpha i subunits and receptor coupling specificity."
      Johnston C.A. , Siderovski D.P.
      Proc. Natl. Acad. Sci. U.S.A.104:2001-2006(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 208-226 IN COMPLEX WITH GNAI1;INTERACTION WITH GNAI1;FUNCTION
    19. 19.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM;RETRACTION
    20. 20.
      "A structural polymorphism of human dopamine D2 receptor, D2(Ser311->Cys)."
      Itokawa M. , Arinami T. , Futamura N. , Hamaguchi H. , Toru M.
      Biochem. Biophys. Res. Commun.196:1369-1375(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CYS-311
    21. 21.
      "Association of a missense change in the D2 dopamine receptor with myoclonus dystonia."
      Klein C. , Brin M.F. , Kramer P. , Sena-Esteves M. , de Leon D. , Doheny D. , Bressman S. , Fahn S. , Breakefield X.O. , Ozelius L.J.
      Proc. Natl. Acad. Sci. U.S.A.96:5173-5176(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT DYT11 ILE-154
    22. 22.
      "Association of the -141C Del variant of the dopamine D2 receptor (DRD2) with positive family history and suicidality in German alcoholics."
      Johann M. , Putzhammer A. , Eichhammer P. , Wodarz N.
      Am. J. Med. Genet. B Neuropsychiatr. Genet.132:46-49(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO ALCOHOLISM
    23. 23.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT DYT11 ILE-154
    24. 24.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT GLU-327
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