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Index > Protein center > Drd2(Gene name) > Rat
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  • Drd2 (Gene name),
  • D(2) dopamine receptor (Protein name ),  DRD2_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Drd2;
    Protein name:
    D(2) dopamine receptor;
    Alternative:
    Dopamine D2 receptor;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Forms homo- and heterooligomers with DRD4. The interaction with DRD4 may modulate agonist-induced downstream signaling (By similarity). Interacts with GNAI1, CADPS and CADPS2 (By similarity). Interacts with GPRASP1, PPP1R9B and CLIC6. Interacts with ARRB2.
    Function:
    Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.
    Subcellular Location:
    Cell membrane Multi-pass membrane protein
    Protein Attributes:
    Sequence length:
    444
    Sequence:
    50:
    MDPLNLSWYD | DDLERQNWSR | PFNGSEGKAD | RPHYNYYAML | LTLLIFIIVF | 
    100:
    GNVLVCMAVS | REKALQTTTN | YLIVSLAVAD | LLVATLVMPW | VVYLEVVGEW | 
    150:
    KFSRIHCDIF | VTLDVMMCTA | SILNLCAISI | DRYTAVAMPM | LYNTRYSSKR | 
    200:
    RVTVMIAIVW | VLSFTISCPL | LFGLNNTDQN | ECIIANPAFV | VYSSIVSFYV | 
    250:
    PFIVTLLVYI | KIYIVLRKRR | KRVNTKRSSR | AFRANLKTPL | KGNCTHPEDM | 
    300:
    KLCTVIMKSN | GSFPVNRRRM | DAARRAQELE | MEMLSSTSPP | ERTRYSPIPP | 
    350:
    SHHQLTLPDP | SHHGLHSNPD | SPAKPEKNGH | AKIVNPRIAK | FFEIQTMPNG | 
    400:
    KTRTSLKTMS | RRKLSQQKEK | KATQMLAIVL | GVFIICWLPF | FITHILNIHC | 
    444:
    DCNIPPVLYS | AFTWLGYVNS | AVNPIIYTTF | NIEFRKAFMK | ILHC
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    SMR:
    Pfam:
    UniGene:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Rat D(2) dopamine receptor
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    E0673m
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    ELISA Kit for Rat D(2) dopamine receptor
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    E0673r
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    ELISA Kit for Rat D(2) dopamine receptor
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    ELISA Kit for Rat D(2) dopamine receptor
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    E0673b
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    ELISA Kit for Rat D(2) dopamine receptor
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    E0673d
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    Packing:
    96T
    CLIA Kit for Rat D(2) dopamine receptor
    Cat.:
    U0673m
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat D(2) dopamine receptor
    Cat.:
    U0673h
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat D(2) dopamine receptor
    Cat.:
    U0673r
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat D(2) dopamine receptor
    Cat.:
    U0673b
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat D(2) dopamine receptor
    Cat.:
    U0673d
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    Packing:
    96T
    Polyclonal Antibody for Rat D(2) dopamine receptor
    Polyclonal Antibody for Rat D(2) dopamine receptor
    Cat.:
    P0673Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat D(2) dopamine receptor
    Polyclonal Antibody for Rat D(2) dopamine receptor
    Cat.:
    P0673Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat D(2) dopamine receptor
    Cat.:
    P0673Rb-h
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat D(2) dopamine receptor
    Monoclonal Antibody for Rat D(2) dopamine receptor
    Monoclonal Antibody for Rat D(2) dopamine receptor
    Monoclonal Antibody for Rat D(2) dopamine receptor
    Monoclonal Antibody for Rat D(2) dopamine receptor
    Protein for Rat D(2) dopamine receptor
    Protein for Rat D(2) dopamine receptor
    Protein for Rat D(2) dopamine receptor
    Protein for Rat D(2) dopamine receptor
    Protein for Rat D(2) dopamine receptor

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.8%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of DRD2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    94-107

    Plasma

    100

    92-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DRD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    94-108%

    96-107%

    93-110%

    97-104%

    EDTA plasma(n=5)

    92-108%

    91-110%

    92-106%

    94-108%

    heparin plasma(n=5)

    95-106%

     

    93-107%

    95-105%

    91-109%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT)
      tissue: Brain.
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Alternative splicing directs the expression of two D2 dopamine receptor isoforms."
      Giros B. , Sokoloff P. , Martres M.-P. , Riou J.-F. , Emorine L.J. , Schwartz J.-C.
      Nature342:923-926(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Multiple D2 dopamine receptors produced by alternative RNA splicing."
      Monsma F.J. Jr. , McVittie L.D. , Gerfen C.R. , Mahan L.C. , Sibley D.R.
      Nature342:926-929(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    5. 5.
      "Two forms of the rat D2 dopamine receptor as revealed by the polymerase chain reaction."
      Rao D.D. , McKelvy J. , Kebabian J. , MacKenzie R.G.
      FEBS Lett.263:18-22(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    6. 6.
      "5'untranslated region of rat pituitary dopamine D2(B) receptor contains a putative CpG island."
      Taylor P.L. , Inglis J.D. , Eidne K.A.
      Submitted (1990-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG)
      strain: Sprague-Dawley.
    7. 7.
      "Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor."
      Miller J.C. , Wang Y. , Filer D.
      Biochem. Biophys. Res. Commun.166:109-112(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 242-270
    8. 8.
      "Cloning of two additional catecholamine receptors from rat brain."
      O'Dowd B.F. , Nguyen T. , Tirpak A. , Jarvie K.R. , Israel Y. , Seeman P. , Niznik H.B.
      FEBS Lett.262:8-12(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 242-270
    9. 9.
      "Contributions of conserved serine residues to the interactions of ligands with dopamine D2 receptors."
      Cox B.A. , Henningsen R.A. , Spanoyannis A. , Neve R.L. , Neve K.A.
      J. Neurochem.59:627-635(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF SER-193; SER-194; SER-197 AND SER-420
    10. 10.
      "Association of the D2 dopamine receptor third cytoplasmic loop with spinophilin, a protein phosphatase-1-interacting protein."
      Smith F.D. , Oxford G.S. , Milgram S.L.
      J. Biol. Chem.274:19894-19900(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PPP1R9B
    11. 11.
      "CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors."
      Griffon N. , Jeanneteau F. , Prieur F. , Diaz J. , Sokoloff P.
      Brain Res. Mol. Brain Res.117:47-57(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CLIC6
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GPRASP1
    13. 13.
      "G protein-coupled receptor kinase-mediated phosphorylation regulates post-endocytic trafficking of the D2 dopamine receptor."
      Namkung Y. , Dipace C. , Javitch J.A. , Sibley D.R.
      J. Biol. Chem.284:15038-15051(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARRB2
    14. 14.
      "Molecular modelling of D2-like dopamine receptors."
      Livingstone C.D. , Strange P.G. , Naylor L.H.
      Biochem. J.287:277-282(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: 3D-STRUCTURE MODELING
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