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Index > Protein center > Dusp1(Gene name) > Rat
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  • Dusp1 (Gene name),
  • Dual specificity protein phosphatase 1 (Protein name ),  DUS1_RAT from NCBI database.
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  • General Annotation
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  • Gene name:
    Dusp1(Cl100;Mkp1;Ptpn16);
    Protein name:
    Dual specificity protein phosphatase 1;
    Alternative:
    Protein-tyrosine phosphatase CL100;Mitogen-activated protein kinase phosphatase 1(MAP kinase phosphatase 1;MKP-1);Protein-tyrosine phosphatase non-receptor type 16;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    367
    Sequence:
    50:
    MVMEVGILDA | GGLRALLRER | AAQCLLLDCR | SFFAFNAGHI | VGSVNVRFST | 
    100:
    IVRRRAKGAM | GLEHIVPNTE | LRGRLLAGAY | HAVVLLDERS | AALDGAKRDG | 
    150:
    TLALAAGALC | REARSTQVFF | LQGGYEAFSA | SCPELCSKQS | TPMGLSLPLS | 
    200:
    TSVPDSAESG | CSSCSTPLYD | QGGPVEILSF | LYLGSAYHAS | RKDMLDALGI | 
    250:
    TALINVSANC | PNHFEGHYQY | KSIPVEDNHK | ADISSWFNEA | IDFIDSIKDA | 
    300:
    GGRVFVHCQA | GISRSATICL | AYLMRTNRVK | LDEAFEFVKQ | RRSIISPNFS | 
    350:
    FMGQLLQFES | QVLAPHCSAE | AGSPAMAVLD | RGTSTTTVFN | FPVSIPVHPT | 
    367:
    NSALNYLQSP | ITTSPSC
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Pfam:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    E9899m
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    E9899r
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    E9899h
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    U9899h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    U9899m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Dual specificity protein phosphatase 1
    Cat.:
    U9899r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Dual specificity protein phosphatase 1
    Cat.:
    P9899Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Dual specificity protein phosphatase 1
    Polyclonal Antibody for Rat Dual specificity protein phosphatase 1
    Cat.:
    P9899Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Dual specificity protein phosphatase 1
    Monoclonal Antibody for Rat Dual specificity protein phosphatase 1
    Monoclonal Antibody for Rat Dual specificity protein phosphatase 1
    Protein for Rat Dual specificity protein phosphatase 1
    Protein for Rat Dual specificity protein phosphatase 1
    Protein for Rat Dual specificity protein phosphatase 1
    Cat.:
    R9899h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    12% SDS-PAGE analysis

               12% SDS-PAGE analysis

    s
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.4%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of DUSP1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    85-98

    Plasma

    93

    88-101

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DUSP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    85-97%

    87-95%

    82-101%

    88-95%

    EDTA plasma(n=5)

    89-98%

    83-105%

    89-97%

    85-100%

    heparin plasma(n=5)

    87-97%

     

    85-101%

    86-99%

    84-102%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Pathways regulating CL100 gene expression in pituitary cells."
      Muda M. , Schlegel W. , Arkinstall S.
      Submitted (1995-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Lung.
    2. 2.
      "MAP kinase phosphatase-1 gene transcription in rat neuroendocrine cells is modulated by a calcium-sensitive block to elongation in the first exon."
      Ryser S. , Tortola S. , van Haasteren G. , Muda M. , Li S. , Schlegel W.
      J. Biol. Chem.276:33319-33327(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: Wistar Furth.
    3. 3.
      "Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene."
      Kwak S.P. , Hakes D.J. , Martell K.J. , Dixon J.E.
      J. Biol. Chem.269:3596-3604(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    4. 4.
      "Mitogen-activated protein kinase phosphatase-1 (MKP-1): >100-fold nocturnal and norepinephrine-induced changes in the rat pineal gland."
      Price D.M. , Chik C.L. , Terriff D. , Weller J. , Humphries A. , Carter D.A. , Klein D.C. , Ho A.K.
      FEBS Lett.577:220-226(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;INDUCTION
    5. 5.
      "Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling."
      Bailey M.J. , Coon S.L. , Carter D.A. , Humphries A. , Kim J.S. , Shi Q. , Gaildrat P. , Morin F. , Ganguly S. , Hogenesch J.B. , Weller J.L. , Rath M.F. , Moller M. , Baler R. , Sugden D. , Rangel Z.G. , Munson P.J. , Klein D.C.
      J. Biol. Chem.284:7606-7622(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;INDUCTION
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