Pikachurin (Protein name
), EGFLA_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (1)
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Gene name:
EGFLAM(AGRINL;AGRNL);
Protein name:
Pikachurin;
Alternative:
EGF-like, fibronectin type-III and laminin G-like domain-containing protein;Agrin-like protein;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Interacts with DAG1 alpha-dystroglycan.
Function:
Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.
Subcellular Location:
Secreted
extracellular space
extracellular matrix
Cell junction
synapse
Detected in the synaptic cleft of the ribbon synapse around the postsynaptic terminals of bipolar cells. Colocalizes with BSN, CTBP2 and DAG1 in photoreceptor synaptic terminals.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
Recovery was determined by spiking various levels of Pikachurin into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
100
95-105
Plasma
103
97-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pikachurin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-105%
93-101%
95-103%
97-105%
Saliva(n=3)
92-97%
96-105%
94-102%
95-106%
heparin plasma(n=5)
95-104%
94-108%
101-108%
96-110%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 5)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 5);VARIANT MET-473
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 874-897;IDENTIFICATION BY MASS SPECTROMETRY