EPO ELISA Kit for Pig(Sus scrofa) | EIAab.comEPO ELISA Kit for Pig(Sus scrofa)

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Index > Protein center > EPO(Gene name) > Pig

EPO (Gene name),

Erythropoietin (Protein name ), EPO_PIG from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

EPO;

Protein name:

Erythropoietin;

Alternative:

Organism:

Pig (Sus scrofa).

General Annotation

Sub Unit:

N/A

Function:

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

194

Sequence:

50:

100:

150:

194:

SPSSATPLRT | FAVDTLCKLF | RNYSNFLRGK | LTLYTGEACR | RRDR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00758

UniGene:

Ssc.15896

String:

P49157

KEGG:

ssc:397249

STRING:

9823.ENSSSCP00000008194

SMR:

P49157

Uniprot:

P49157

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ELISA Kit for Pig Erythropoietin

Cat.:

E0028r

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96T

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ELISA Kit for Pig Erythropoietin

Cat.:

E0028h

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96T

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ELISA Kit for Pig Erythropoietin

Cat.:

E0028d

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ELISA Kit for Pig Erythropoietin

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E0028p

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ELISA Kit for Pig Erythropoietin

Cat.:

E0028m

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ELISA Kit for Pig Erythropoietin

Cat.:

E0028b

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ELISA Kit for Pig Erythropoietin

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CLIA Kit for Pig Erythropoietin

Cat.:

U0028b

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96T

CLIA Kit for Pig Erythropoietin

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CLIA Kit for Pig Erythropoietin

Cat.:

U0028m

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CLIA Kit for Pig Erythropoietin

Cat.:

U0028h

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Looking for manual.

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96T

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CLIA Kit for Pig Erythropoietin

Cat.:

U0028d

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Looking for manual.

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96T

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CLIA Kit for Pig Erythropoietin

Cat.:

U0028p

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Looking for manual.

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96T

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CLIA Kit for Pig Erythropoietin

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U0028r

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Polyclonal Antibody for Pig Erythropoietin

Cat.:

P0028Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig Erythropoietin

Cat.:

P0028Rb-r

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40ug/0.2ml

Polyclonal Antibody for Pig Erythropoietin

Cat.:

P0028Rb-m

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40ug/0.2ml

Polyclonal Antibody for Pig Erythropoietin

Monoclonal Antibody for Pig Erythropoietin

Protein for Pig Erythropoietin

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-99%	94-98%	95-100%	100-105%
EDTA plasma(n=5)	91-95%	93-99%	89-94%	96-100%
heparin plasma(n=5)	93-96%	99-106%	97-100%	94-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-102%	95-101%	98-104%	97-105%
EDTA plasma(n=5)	96-99%	97-104%	100-105%	89-94%
heparin plasma(n=5)	91-96%	95-100%	99-107%	101-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Identification and characterization of neurotrophic factors in porcine small intestinal submucosa"

KJ Yang, KC Park, H Choi, et al.

From:Tissue Engineering and Regenerative Medicine

References

1.

"The porcine erythropoietin gene: cDNA sequence, genomic sequence and expression analyses in piglets."
David R.B. , Blom A.K. , Sjaastad O.V. , Harbitz I.
Domest. Anim. Endocrinol.20:137-147(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
strain: Norwegian Landrace.
tissue: Kidney.
2.

"Erythropoietin structure-function relationships: high degree of sequence homology among mammals."
Wen D. , Boissel J.-P.R. , Tracy T.E. , Gruninger R.H. , Mulcahy L.S. , Czelusniak J. , Goodman M. , Bunn H.F.
Blood82:1507-1516(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-194
tissue: Kidney.

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