FGF1 ELISA Kit for Pig(Sus scrofa) | EIAab.comFGF1 ELISA Kit for Pig(Sus scrofa)

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Index > Protein center > FGF1(Gene name) > Pig

FGF1 (Gene name),

Heparin-binding growth factor 1 (Protein name ), FGF1_PIG from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

FGF1(FGF-1);

Protein name:

Heparin-binding growth factor 1(HBGF-1);

Alternative:

Alpha-endothelial cell growth factor(ECGF);Acidic fibroblast growth factor(aFGF);Heparin-binding growth factor 1(HBGF-1);

Organism:

Pig (Sus scrofa).

General Annotation

Sub Unit:

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13.

Function:

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Subcellular Location:

Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

Protein Attributes:

Sequence length:

152

Sequence:

50:

100:

150:

152:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P20002

UniGene:

Ssc.16246

String:

P20002

Pfam:

PF00167

STRING:

9823.ENSSSCP00000015321

Uniprot:

P20002

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ELISA Kit for Pig HBGF-1

Cat.:

E0032h

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96T

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ELISA Kit for Pig HBGF-1

Cat.:

E0032m

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ELISA Kit for Pig HBGF-1

Cat.:

E0032r

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ELISA Kit for Pig HBGF-1

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E0032p

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ELISA Kit for Pig HBGF-1

Cat.:

E0032b

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ELISA Kit for Pig HBGF-1

Cat.:

E0032d

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ELISA Kit for Pig HBGF-1

Cat.:

E0032c

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CLIA Kit for Pig HBGF-1

Cat.:

U0032r

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CLIA Kit for Pig HBGF-1

Cat.:

U0032p

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96T

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CLIA Kit for Pig HBGF-1

Cat.:

U0032m

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Looking for manual.

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Packing:

96T

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CLIA Kit for Pig HBGF-1

Cat.:

U0032h

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Looking for manual.

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96T

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CLIA Kit for Pig HBGF-1

Cat.:

U0032d

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96T

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CLIA Kit for Pig HBGF-1

Cat.:

U0032b

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96T

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CLIA Kit for Pig HBGF-1

Cat.:

U0032c

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Polyclonal Antibody for Pig HBGF-1

Cat.:

P0032Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig HBGF-1

Cat.:

P0032Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig HBGF-1

Cat.:

P0032Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig HBGF-1

Monoclonal Antibody for Pig HBGF-1

Protein for Pig HBGF-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of FGF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	93-97
Plasma	97	96-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-100%	97-104%	95-98%	91-96%
EDTA plasma(n=5)	89-93%	102-105%	94-97%	93-98%
heparin plasma(n=5)	91-95%	96-99%	106-108%	87-93%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Amplification and sequencing of mRNA encoding acidic fibroblast growth factor (aFGF) from porcine heart."
Schmidt M. , Sharma H.S. , Schott R.J. , Schaper W.
Biochem. Biophys. Res. Commun.180:853-859(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Heart.
2.

"Isolation of heparin-binding growth factors from bovine, porcine and canine hearts."
Quinkler W. , Maasberg M. , Bernotat-Danielowski S. , Luethe N. , Sharma H.S. , Schaper W.
Eur. J. Biochem.181:67-73(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-41

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