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Index > Protein center > Fgf1(Gene name) > Rat
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  • Fgf1 (Gene name),
  • Heparin-binding growth factor 1 (Protein name ),  FGF1_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Fgf1(Fgf-1;Fgfa);
    Protein name:
    Heparin-binding growth factor 1(HBGF-1);
    Alternative:
    Acidic fibroblast growth factor(aFGF);Heparin-binding growth factor 1(HBGF-1);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13.
    Function:
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
    Subcellular Location:
    Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    Protein Attributes:
    Sequence length:
    155
    Sequence:
    50:
    MAEGEITTFA | ALTERFNLPL | GNYKKPKLLY | CSNGGHFLRI | LPDGTVDGTR | 
    100:
    DRSDQHIQLQ | LSAESAGEVY | IKGTETGQYL | AMDTEGLLYG | SQTPNEECLF | 
    150:
    LERLEENHYN | TYTSKKHAEK | NWFVGLKKNG | SCKRGPRTHY | GQKAILFLPL | 
    155:
    PVSSD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    UniGene:
    String:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat HBGF-1
    Cat.:
    E0032c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat HBGF-1
    Cat.:
    U0032c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat HBGF-1
    Polyclonal Antibody for Rat HBGF-1
    Polyclonal Antibody for Rat HBGF-1
    Polyclonal Antibody for Rat HBGF-1
    Polyclonal Antibody for Rat HBGF-1
    Cat.:
    P0032Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat HBGF-1
    Cat.:
    P0032Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat HBGF-1
    Cat.:
    P0032Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Monoclonal Antibody for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1
    Protein for Rat HBGF-1

    R&D Technical Data

    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.8%

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    Recovery

    Recovery was determined by spiking various levels of FGF1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    93-97

    Plasma

    97

    96-102

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-100%

    97-104%

    95-98%

    91-96%

    EDTA plasma(n=5)

    89-93%

    102-105%

    94-97%

    93-98%

    heparin plasma(n=5)

    91-95%

     

    96-99%

    106-108%

    87-93%

     

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    References
    1. 1.
      "The nucleotide sequence of rat heparin binding growth factor 1 (HBGF-1)."
      Goodrich S. , Yan G.C. , Bahrenburg K. , Mansson P.E.
      Nucleic Acids Res.17:2867-2867(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Astrocytes produce and secrete FGF-1, which promotes the production of apoE-HDL in a manner of autocrine action."
      Ito J. , Nagayasu Y. , Lu R. , Kheirollah A. , Hayashi M. , Yokoyama S.
      J. Lipid Res.46:679-686(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    3. 3.
      "Structure of rat acidic fibroblast growth factor at 1.4 A resolution."
      Kulahin N. , Kiselyov V. , Kochoyan A. , Kristensen O. , Kastrup J.S. , Berezin V. , Bock E. , Gajhede M.
      Acta Crystallogr. F63:65-68(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 22-155
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 22-153
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