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  • FSHB (Gene name),
  • Follitropin subunit beta (Protein name ),  FSHB_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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    • Gene name:
    FSHB;
    Protein name:
    Follitropin subunit beta;
    Alternative:
    Follitropin beta chain;Follicle-stimulating hormone beta subunit(FSH-B;FSH-beta);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
    Function:
    Stimulates development of follicle and spermatogenesis in the reproductive organs.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    129
    Sequence:
    50:
    MKTLQFFFLF | CCWKAICCNS | CELTNITIAI | EKEECRFCIS | INTTWCAGYC | 
    100:
    YTRDLVYKDP | ARPKIQKTCT | FKELVYETVR | VPGCAHHADS | LYTYPVATQC | 
    129:
    HCGKCDSDST | DCTVRGLGPS | YCSFGEMKE
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    Hs.36975
    MIM:
    136530
    STRING:
    9606.ENSP00000254122
    Pfam:
    PF00007
    String:
    P01225
    KEGG:
    hsa:2488
    SMR:
    P01225
    Uniprot:
    P01225
     
    FOR
    ELISA Kit for Human Follitropin subunit beta
    Cat.:
    E0830h
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    96T
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    ELISA Kit for Human Follitropin subunit beta
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    E0830m
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    ELISA Kit for Human Follitropin subunit beta
    Cat.:
    E0830r
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    ELISA Kit for Human Follitropin subunit beta
    Cat.:
    E0830b
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    ELISA Kit for Human Follitropin subunit beta
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    E0830s
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    ELISA Kit for Human Follitropin subunit beta
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    ELISA Kit for Human Follitropin subunit beta
    Cat.:
    E0830p
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    CLIA Kit for Human Follitropin subunit beta
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    CLIA Kit for Human Follitropin subunit beta
    Cat.:
    U0830r
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    Packing:
    96T
    CLIA Kit for Human Follitropin subunit beta
    Cat.:
    U0830b
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    Packing:
    96T
    CLIA Kit for Human Follitropin subunit beta
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    U0830h
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    Packing:
    96T
    CLIA Kit for Human Follitropin subunit beta
    Cat.:
    U0830m
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    CLIA Kit for Human Follitropin subunit beta
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    Packing:
    96T
    Polyclonal Antibody for Human Follitropin subunit beta
    Polyclonal Antibody for Human Follitropin subunit beta
    Cat.:
    P0830Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Follitropin subunit beta
    Cat.:
    P0830Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Follitropin subunit beta
    Cat.:
    P0830Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Follitropin subunit beta
    Polyclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Monoclonal Antibody for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta
    Protein for Human Follitropin subunit beta

    R&D Technical Data

    12% SDS-PAGE analysis

             12% SDS-PAGE analysis

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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11."
      Watkins P.C. , Eddy R. , Beck A.K. , Vellucci V. , Leverone B. , Tanzi R.E. , Gusella J.F. , Shows T.B.
      DNA6:205-212(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Expression of biologically active human follitropin in Chinese hamster ovary cells."
      Keene J.L. , Matzuk M.M. , Otani T. , Fauser B.C.J.M. , Galway A.B. , Hsueh A.J.W. , Boime I.
      J. Biol. Chem.264:4769-4775(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Human follicle-stimulating hormone beta-subunit gene encodes multiple messenger ribonucleic acids."
      Jameson J.L. , Becker C.B. , Lindell C.M. , Habener J.F.
      Mol. Endocrinol.2:806-815(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "Follicle-stimulating hormone (FSHB) beta subunit genes."
      Akbari Eidgahi M.R. , Shaebani A.A. , Nasr R.
      Submitted (2007-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    8. 8.
      "A reevaluation of the amino acid sequence of human follitropin beta-subunit."
      Shome B. , Parlow A.F. , Liu W.K. , Nahm H.S. , Wen T. , Ward D.N.
      J. Protein Chem.7:325-339(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-129
    9. 9.
      "Amino acid sequence of the beta subunit of follicle-stimulating hormone from human pituitary glands."
      Saxena B.B. , Rathnam P.
      J. Biol. Chem.251:993-1005(1976) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 19-129
    10. 10.
      "Human follicle stimulating hormone: first proposal for the amino acid sequence of the hormone-specific, beta subunit (hFSHb)."
      Shome B. , Parlow A.F.
      J. Clin. Endocrinol. Metab.39:203-205(1974) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 19-129
    11. 11.
      "Studies on the disulfide bonds in human pituitary follicle-stimulating hormone."
      Fujiki Y. , Rathnam P. , Saxena B.B.
      Biochim. Biophys. Acta624:428-435(1980) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PRELIMINARY ASSIGNMENT OF DISULFIDE BONDS
    12. 12.
      "Assignment of the complete disulphide bridge pattern in the human recombinant follitropin beta-chain."
      Amoresano A. , Orru S. , Siciliano R.A. , De Luca E. , Napoleoni R. , Sirna A. , Pucci P.
      Biol. Chem.382:961-968(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
    13. 13.
      "Three-dimensional structure of human follicle-stimulating hormone."
      Fox K.M. , Dias J.A. , Van Roey P.
      Mol. Endocrinol.15:378-389(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF MUTANT ALA-27
    14. 14.
      "Structure of human follicle-stimulating hormone in complex with its receptor."
      Fan Q.R. , Hendrickson W.A.
      Nature433:269-277(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.92 ANGSTROMS) OF 19-129 IN COMPLEX WITH CGA AND FSHR;DISULFIDE BONDS;GLYCOSYLATION AT ASN-25 AND ASN-42
    15. 15.
      "Primary amenorrhoea and infertility due to a mutation in the beta-subunit of follicle-stimulating hormone."
      Matthews C.H. , Borgato S. , Beck-Peccoz P. , Adams M. , Tone Y. , Gambino G. , Casagrande S. , Tedeschini G. , Benedetti A. , Chatterjee V.K.K.
      Nat. Genet.5:83-86(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN IFSHD
    16. 16.
      "Delayed puberty and hypogonadism caused by mutations in the follicle-stimulating hormone beta-subunit gene."
      Layman L.C. , Lee E.-J. , Peak D.B. , Namnoum A.B. , Vu K.V. , van Lingen B.L. , Gray M.R. , McDonough P.G. , Reindollar R.H. , Jameson J.L.
      N. Engl. J. Med.337:607-611(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IFSHD GLY-69
    17. 17.
      "Isolated deficiency of follicle-stimulating hormone re-revisited."
      Matthews C. , Chatterjee V.K.
      N. Engl. J. Med.337:642-642(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN IFSHD
    18. 18.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ILE-20
    19. 19.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
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