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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.7%
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Recovery
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Recovery was determined by spiking various levels of G6PD1into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
106
95-108
Plasma
104
94-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of G6PD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
99-112%
100-110%
102-109%
97-113%
EDTA plasma(n=5)
96-112%
98-108%
99-107%
96-111%
heparin plasma(n=5)
95-1114%
97-110%
98-109%
99-107%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 57-71; 175-191 AND 247-257;IDENTIFICATION BY MASS SPECTROMETRY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-214 AND 352-454
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-507;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]