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Index > Protein center > Gpx1(Gene name) > Mouse
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  • Gpx1 (Gene name),
  • Glutathione peroxidase 1 (Protein name ),  GPX1_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (1)
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  • Gene name:
    Gpx1;
    Protein name:
    Glutathione peroxidase 1(GPx-1;GSHPx-1);
    Alternative:
    Selenium-dependent glutathione peroxidase 1;Cellular glutathione peroxidase;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homotetramer.
    Function:
    Protects the hemoglobin in erythrocytes from oxidative breakdown.
    Subcellular Location:
    Cytoplasm
    Protein Attributes:
    Sequence length:
    201
    Sequence:
    50:
    MCAARLSAAA | QSTVYAFSAR | PLTGGEPVSL | GSLRGKVLLI | ENVASLUGTT | 
    100:
    IRDYTEMNDL | QKRLGPRGLV | VLGFPCNQFG | HQENGKNEEI | LNSLKYVRPG | 
    150:
    GGFEPNFTLF | EKCEVNGEKA | HPLFTFLRNA | LPTPSDDPTA | LMTDPKYIIW | 
    200:
    SPVCRNDIAW | NFEKFLVGPD | GVPVRRYSRR | FRTIDIEPDI | ETLLSQQSGN | 
    201:
    S
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    UniGene:
    SMR:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse GPx-1
    Cat.:
    E0295r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse GPx-1
    Cat.:
    E0295b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse GPx-1
    Cat.:
    E0295h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse GPx-1
    Cat.:
    E0295m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse GPx-1
    Cat.:
    E0295p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Mouse GPx-1
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse GPx-1
    Cat.:
    U0295h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse GPx-1
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse GPx-1
    Cat.:
    U0295b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse GPx-1
    Cat.:
    U0295r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse GPx-1
    Cat.:
    U0295m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse GPx-1
    Cat.:
    U0295p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse GPx-1
    Cat.:
    P0295Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse GPx-1
    Polyclonal Antibody for Mouse GPx-1
    Polyclonal Antibody for Mouse GPx-1
    Cat.:
    P0295Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse GPx-1
    Polyclonal Antibody for Mouse GPx-1
    Cat.:
    P0295Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse GPx-1
    Monoclonal Antibody for Mouse GPx-1
    Monoclonal Antibody for Mouse GPx-1
    Monoclonal Antibody for Mouse GPx-1
    Monoclonal Antibody for Mouse GPx-1
    Monoclonal Antibody for Mouse GPx-1
    Protein for Mouse GPx-1
    Protein for Mouse GPx-1
    Protein for Mouse GPx-1
    Protein for Mouse GPx-1
    Protein for Mouse GPx-1
    Protein for Mouse GPx-1

    R&D Technical Data

    s

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    s

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.8%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of GPX1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    93-105

    Plasma

    101

    95-107

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-105%

    95-102%

    92-108%

    90-110%

    EDTA plasma(n=5)

    91-110%

    93-107%

    94-108%

    97-105%

    heparin plasma(n=5)

    96-106%

     

    96-104%

    95-106%

    93-109%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the 'termination' codon, TGA."
      Chambers I. , Frampton J. , Goldfarb P. , Affara N. , McBain W. , Harrison P.R.
      EMBO J.5:1221-1227(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];SELENOCYSTEINE AT SEC-47
      tissue: Erythrocyte.
    2. 2.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      strain: NOD.
      tissue: Bone marrow.
      tissue: Embryonic liver.
      tissue: Kidney.
      tissue: Liver.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6.
      tissue: Brain.
    4. 4.
      "A human cDNA sequence for a novel glutathione peroxidase-related selenopeptide, GPRP."
      Dunn D.K. , Howells D.D. , Richardson J. , Goldfarb P.S.
      Nucleic Acids Res.17:6390-6390(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Liver.
    5. 5.
      "SelT, SelW, SelH, and Rdx12: genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family."
      Dikiy A. , Novoselov S.V. , Fomenko D.E. , Sengupta A. , Carlson B.A. , Cerny R.L. , Ginalski K. , Grishin N.V. , Hatfield D.L. , Gladyshev V.N.
      Biochemistry46:6871-6882(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MIEN1
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    7. 7.
      "Knockout of SOD1 promotes conversion of selenocysteine to dehydroalanine in murine hepatic GPX1 protein."
      Wang S.K. , Weaver J.D. , Zhang S. , Lei X.G.
      Free Radic. Biol. Med.51:197-204(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: BIOPHYSICOCHEMICAL PROPERTIES;SELENOCYSTEINE AT SEC-47;PARTIAL LOSS OF SELENIUM IN ABSENCE OF SOD1;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Liver.
    8. 8.
      "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
      Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-62; LYS-86; LYS-112 AND LYS-146;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    9. 9.
      "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J. , Newman J.C. , Held J.M. , Cusack M.P. , Sorensen D.J. , Li B. , Schilling B. , Mooney S.D. , Kahn C.R. , Verdin E. , Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A.110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-62; LYS-86; LYS-112; LYS-119 AND LYS-146;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
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