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s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤8.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.8%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Recovery was determined by spiking various levels of GPX1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
93-105
Plasma
101
95-107
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
93-105%
95-102%
92-108%
90-110%
EDTA plasma(n=5)
91-110%
93-107%
94-108%
97-105%
heparin plasma(n=5)
96-106%
96-104%
95-106%
93-109%
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];TISSUE SPECIFICITY
"Functional analysis of the 5'-flanking region of the rat glutathione peroxidase gene." Suemizu H.
Submitted (1997-06) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 7-52;SELENOCYSTEINE AT SEC-47
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 96-112 AND 165-176;IDENTIFICATION BY MASS SPECTROMETRY