GZMB ELISA Kit for Human(Homo sapiens) | EIAab.comGZMB ELISA Kit for Human(Homo sapiens)

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Index > Protein center > GZMB(Gene name) > Human

GZMB (Gene name),

Granzyme B (Protein name ), GRAB_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

GZMB(CGL1;CSPB;CTLA1;GRB);

Protein name:

Granzyme B;

Alternative:

CTLA-1;C11;Cytotoxic T-lymphocyte proteinase 2(Lymphocyte protease);Cathepsin G-like 1(CTSGL1);Granzyme-2;Fragmentin-2;SECT;Human lymphocyte protein(HLP);T-cell serine protease 1-3E;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Subcellular Location:

Cytoplasmic granule Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Protein Attributes:

Sequence length:

247

Sequence:

50:

100:

150:

200:

247:

GDSGGPLVCN | KVAQGIVSYG | RNNGMPPRAC | TKVSSFVHWI | KKTMKRY

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000216341

Pfam:

PF00089

UniGene:

Hs.1051

SMR:

P10144

KEGG:

hsa:3002

String:

P10144

MIM:

123910

Uniprot:

P10144

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ELISA Kit for Human Granzyme B

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E0600h

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96T

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ELISA Kit for Human Granzyme B

Cat.:

E0600r

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96T

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ELISA Kit for Human Granzyme B

Cat.:

E0600m

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96T

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CLIA Kit for Human Granzyme B

Cat.:

U0600m

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96T

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CLIA Kit for Human Granzyme B

Cat.:

U0600r

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96T

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CLIA Kit for Human Granzyme B

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U0600h

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96T

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Polyclonal Antibody for Human Granzyme B

Cat.:

P0600Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Granzyme B

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P0600Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Granzyme B

Cat.:

P0600Rb-m

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40ug/0.2ml

Monoclonal Antibody for Human Granzyme B

Protein for Human Granzyme B

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of Granzyme B into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	101	85-109
Plasma	99	94-110

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granzyme B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	80-96%	82-10%	87-98%	96-103%
EDTA plasma(n=5)	90-105%	93-102%	86-105%	84-102%
heparin plasma(n=5)	85-99%	85-94%	84-101%	85-101%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Study of effect of inhaled versus oral corticosteroids on sputum granzyme B in patients with moderate persistent bronchial asthma"

W Shoeib, M Alsalahy, M Almahdy, et al.

From:Egyptian Journal of Chest Diseases and Tuberculosis

References

1.

"Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes."
Schmid J. , Weissmann C.
J. Immunol.139:250-256(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT GLN-55
2.

"Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes."
Caputo A. , Fahey D. , Lloyd C. , Vozab R. , McCairns E. , Rowe P.B.
J. Biol. Chem.263:6363-6369(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT ALA-94
3.

"Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes."
Trapani J.A. , Klein J.L. , White P.C. , Dupont B.
Proc. Natl. Acad. Sci. U.S.A.85:6924-6928(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT GLN-55
4.

"Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes."
Klein J.L. , Shows T.B. , Dupont B. , Trapani J.A.
Genomics5:110-117(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLN-55
5.

"Nucleotide sequence and genomic organization of a human T lymphocyte serine protease gene."
Caputo A. , Sauer D.E. , Rowe P.B.
J. Immunol.145:737-744(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT GLN-55
6.

"Structural organization of the hCTLA-1 gene encoding human granzyme B."
Haddad P. , Clement M.-V. , Bernard O. , Larsen C.-J. , Degos L. , Sasportes M. , Mathieu-Mahul D.
Gene87:265-271(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS GLN-55 AND ALA-94
7.

"The DNA sequence and analysis of human chromosome 14."
Heilig R. , Eckenberg R. , Petit J.-L. , Fonknechten N. , Da Silva C. , Cattolico L. , Levy M. , Barbe V. , De Berardinis V. , Ureta-Vidal A. , Pelletier E. , Vico V. , Anthouard V. , Rowen L. , Madan A. , Qin S. , Sun H. , Du H. , more...
Nature421:601-607(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS GLN-55 AND HIS-247
tissue: Pancreas.
9.

"Isolation of a cDNA clone encoding a novel form of granzyme B from human NK cells and mapping to chromosome 14."
Dahl C.A. , Bach F.H. , Chan W. , Huebner K. , Russo G. , Croce C.M. , Herfurth T. , Cairns J.S.
Hum. Genet.84:465-470(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-23
10.

"Characterization of three serine esterases isolated from human IL-2 activated killer cells."
Hameed A. , Lowrey D.M. , Lichtenheld M. , Podack E.R.
J. Immunol.141:3142-3147(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-40;CHARACTERIZATION
11.

"Characterization of granzymes A and B isolated from granules of cloned human cytotoxic T lymphocytes."
Kraehenbuhl O. , Rey C. , Jenne D.E. , Lanzavecchia A. , Groscurth P. , Carrel S. , Tschopp J.
J. Immunol.141:3471-3477(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-40;CHARACTERIZATION
12.

"Human granzyme B degrades aggrecan proteoglycan in matrix synthesized by chondrocytes."
Froelich C.J. , Zhang X. , Turbov J. , Hudig D. , Winkler U. , Hanna W.L.
J. Immunol.151:7161-7171(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-39;CATALYTIC ACTIVITY;ENZYME REGULATION;SUBCELLULAR LOCATION
tissue: Lymphocyte.
13.

"Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity."
Poe M. , Blake J.T. , Boulton D.A. , Gammon M. , Sigal N.H. , Wu J.K. , Zweerink H.J.
J. Biol. Chem.266:98-103(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-38
14.

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z. , Henzel W.J.
Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-33
15.

"Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue."
Estebanez-Perpina E. , Fuentes-Prior P. , Belorgey D. , Braun M. , Kiefersauer R. , Maskos K. , Huber R. , Rubin H. , Bode W.
Biol. Chem.381:1203-1214(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 21-247
16.

"The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1."
Rotonda J. , Garcia-Calvo M. , Bull H.G. , Geissler W.M. , McKeever B.M. , Willoughby C.A. , Thornberry N.A. , Becker J.W.
Chem. Biol.8:357-368(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 21-247
17.

"Catalog of 680 variations among eight cytochrome p450 (CYP) genes, nine esterase genes, and two other genes in the Japanese population."
Saito S. , Iida A. , Sekine A. , Kawauchi S. , Higuchi S. , Ogawa C. , Nakamura Y.
J. Hum. Genet.48:249-270(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS GLN-55 AND HIS-247

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