Gzmb ELISA Kit for Rat(Rattus norvegicus) | EIAab.comGzmb ELISA Kit for Rat(Rattus norvegicus)

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Gzmb (Gene name),

Granzyme B (Protein name ), GRAB_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Gzmb;

Protein name:

Granzyme B;

Alternative:

Natural killer cell protease 1;Fragmentin;RNKP-1;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

248

Sequence:

50:

100:

150:

200:

248:

RGDSGGPLVC | KKVAAGIVSY | GQNDGSTPRA | FTKVSTFLSW | IKKTMKKS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P18291

KEGG:

rno:171528

Pfam:

PF00089

KEGG:

rno:100361468

UniGene:

Rn.132115

SMR:

P18291

STRING:

10116.ENSRNOP00000040429

Uniprot:

P18291

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ELISA Kit for Rat Granzyme B

Cat.:

E0600h

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96T

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ELISA Kit for Rat Granzyme B

Cat.:

E0600r

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96T

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ELISA Kit for Rat Granzyme B

Cat.:

E0600m

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CLIA Kit for Rat Granzyme B

Cat.:

U0600m

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96T

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CLIA Kit for Rat Granzyme B

Cat.:

U0600r

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96T

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CLIA Kit for Rat Granzyme B

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U0600h

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96T

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Polyclonal Antibody for Rat Granzyme B

Cat.:

P0600Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Granzyme B

Cat.:

P0600Rb-h

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40ug/0.2ml

Polyclonal Antibody for Rat Granzyme B

Cat.:

P0600Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat Granzyme B

Protein for Rat Granzyme B

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of Granzyme B into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	101	85-109
Plasma	99	94-110

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granzyme B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	80-96%	82-10%	87-98%	96-103%
EDTA plasma(n=5)	90-105%	93-102%	86-105%	84-102%
heparin plasma(n=5)	85-99%	85-94%	84-101%	85-101%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"RNKP-1, a novel natural killer-associated serine protease gene cloned from RNK-16 cytotoxic lymphocytes."
Zunino S.J. , Bleackley R.C. , Martinez J. , Hudig D.
J. Immunol.144:2001-2009(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: T-cell.
2.

"Purification of a factor from the granules of a rat natural killer cell line (RNK) that reduces tumor cell growth and changes tumor morphology. Molecular identity with a granule serine protease (RNKP-1)."
Sayers T.J. , Wiltrout T.A. , Sowder R. , Munger W.L. , Smyth M.J. , Henderson L.E.
J. Immunol.148:292-300(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-53
3.

"A natural killer cell granule protein that induces DNA fragmentation and apoptosis."
Shi L. , Kraut R.P. , Aebersold R. , Greenberg A.H.
J. Exp. Med.175:553-566(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
4.

"The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity."
Waugh S.M. , Harris J.L. , Fletterick R. , Craik C.S.
Nat. Struct. Biol.7:762-765(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 21-248 IN COMPLEX WITH E.COLI ECOTIN;ACTIVE SITE;DISULFIDE BONDS

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