CD74 ELISA Kit for Human(Homo sapiens) | EIAab.comCD74 ELISA Kit for Human(Homo sapiens)

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CD74 (Gene name),

HLA class II histocompatibility antigen gamma chain (Protein name ), HG2A_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CD74(DHLAG);

Protein name:

HLA class II histocompatibility antigen gamma chain;

Alternative:

Ia antigen-associated invariant chain(Ii);HLA-DR antigens-associated invariant chain;p33;

CD:

CD74;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homotrimer. In the endoplasmic reticulum (ER) it forms an heterononameric MHC II-Ii complex: 3 MHC class II molecules (heterodimers of an alpha and a beta subunit) bind to the CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system, the CD74 component undergoes sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide) attached to the MHC class II molecule (alpha-beta-CLIP complex). This processed complex interacts with HLA_DM and HLA_DO heterodimers in order to release CLIP and facilitate the binding of antigenic peptides to the MHC class II molecules.

Function:

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

Subcellular Location:

Cell membrane Single-pass type II membrane protein Endoplasmic reticulum membrane Golgi apparatus trans-Golgi network Endosome Lysosome Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.

Protein Attributes:

Sequence length:

296

Sequence:

50:

100:

150:

200:

250:

296:

WCVFPNGTEV | PNTRSRGHHN | CSESLELEDP | SSGLGVTKQD | LGPVPM

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:972

SMR:

P04233

UniGene:

Hs.436568

Pfam:

PF09307

STRING:

9606.ENSP00000009530

MIM:

142790

String:

P04233

Uniprot:

P04233

FOR

ELISA Kit for Human HLA class II histocompatibility antigen gamma chain

Cat.:

E13627h

Price:

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Manual:

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MSDS:

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Packing:

96T

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ELISA Kit for Human HLA class II histocompatibility antigen gamma chain

Cat.:

E13627r

Price:

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MSDS:

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Packing:

96T

ELISA Kit for Human HLA class II histocompatibility antigen gamma chain

Cat.:

E13627m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human HLA class II histocompatibility antigen gamma chain

MSDS:

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CLIA Kit for Human HLA class II histocompatibility antigen gamma chain

Cat.:

U13627h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human HLA class II histocompatibility antigen gamma chain

Cat.:

U13627m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

Polyclonal Antibody for Human HLA class II histocompatibility antigen gamma chain

Monoclonal Antibody for Human HLA class II histocompatibility antigen gamma chain

Protein for Human HLA class II histocompatibility antigen gamma chain

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-98
Plasma	97	93-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	90-101%	93-101%	92-106%
EDTA plasma(n=5)	89-97%	90-102%	89-102%	90-106%
heparin plasma(n=5)	93-104%	94-108%	101-109%	93-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure."
Claesson L. , Larhammar D. , Rask L. , Peterson P.A.
Proc. Natl. Acad. Sci. U.S.A.80:7395-7399(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
2.

"The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity."
Strubin M. , Mach B. , Long E.O.
EMBO J.3:869-872(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
3.

"Structure of the human gene encoding the invariant gamma-chain of class II histocompatibility antigens."
Kudo J. , Chao L.-Y. , Narni F. , Saunders G.F.
Nucleic Acids Res.13:8827-8841(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 2)
4.

"Structure of the human Ia-associated invariant (gamma)-chain gene: identification of 5' sequences shared with major histocompatibility complex class II genes."
O'Sullivan D.M. , Larhammar D. , Wilson M.C. , Peterson P.A. , Quaranta V.
Proc. Natl. Acad. Sci. U.S.A.83:4484-4488(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORMS 1 AND 2)
tissue: Liver.
5.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2004-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
6.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
tissue: Heart.
tissue: Synovium.
7.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)
tissue: B-cell.
tissue: Tonsil.
10.

Bienvenut W.V.
Submitted (2005-06) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-35; 81-94; 171-179 AND 273-288;IDENTIFICATION BY MASS SPECTROMETRY
tissue: B-cell lymphoma.
11.

"HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides."
Riberdy J.M. , Newcomb J.R. , Surman M.J. , Barbosa J.A. , Cresswell P.
Nature360:474-477(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 97-120
12.

"Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21)."
Charest A. , Lane K. , McMahon K. , Park J. , Preisinger E. , Conroy H. , Housman D.
Genes Chromosomes Cancer37:58-71(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISEASE;CHROMOSOMAL TRANSLOCATION WITH ROS1
13.

"MIF signal transduction initiated by binding to CD74."
Leng L. , Metz C.N. , Fang Y. , Xu J. , Donnelly S. , Baugh J. , Delohery T. , Chen Y. , Mitchell R.A. , Bucala R.
J. Exp. Med.197:1467-1476(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MIF
14.

"MHC class II transport at a glance."
Berger A.C. , Roche P.A.
J. Cell Sci.122:1-4(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
15.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136
tissue: Liver.
16.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
17.

"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
Halim A. , Nilsson J. , Ruetschi U. , Hesse C. , Larson G.
Mol. Cell. Proteomics0:0-0(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-203;STRUCTURE OF CARBOHYDRATES;IDENTIFICATION BY MASS SPECTROMETRY
18.

"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
Halim A. , Ruetschi U. , Larson G. , Nilsson J.
J. Proteome Res.12:573-584(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-203 AND SER-281;IDENTIFICATION BY MASS SPECTROMETRY
19.

"The structure of an intermediate in class II MHC maturation: CLIP bound to HLA-DR3."
Ghosh P. , Amaya M. , Mellins E. , Wiley D.C.
Nature378:457-462(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 103-117 (CLIP) IN COMPLEX WITH HLA-DRA/HLA-DRB1 HETERODIMER
20.

"Structure of a trimeric domain of the MHC class II-associated chaperonin and targeting protein Ii."
Jasanoff A. , Wagner G. , Wiley D.C.
EMBO J.17:6812-6818(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 134-208
21.

"Crystal structure of MHC class II-associated p41 Ii fragment bound to cathepsin L reveals the structural basis for differentiation between cathepsins L and S."
Guncar G. , Pungercic G. , Klemencic I. , Turk V. , Turk D.
EMBO J.18:793-803(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 210-274;GLYCOSYLATION AT ASN-256;DISULFIDE BONDS

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