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Index > Protein center > CD74(Gene name) > Human
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  • CD74 (Gene name),
  • HLA class II histocompatibility antigen gamma chain (Protein name ),  HG2A_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    CD74(DHLAG);
    Protein name:
    HLA class II histocompatibility antigen gamma chain;
    Alternative:
    Ia antigen-associated invariant chain(Ii);HLA-DR antigens-associated invariant chain;p33;
    CD:
    CD74;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homotrimer. In the endoplasmic reticulum (ER) it forms an heterononameric MHC II-Ii complex: 3 MHC class II molecules (heterodimers of an alpha and a beta subunit) bind to the CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system, the CD74 component undergoes sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide) attached to the MHC class II molecule (alpha-beta-CLIP complex). This processed complex interacts with HLA_DM and HLA_DO heterodimers in order to release CLIP and facilitate the binding of antigenic peptides to the MHC class II molecules.
    Function:
    Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
    Subcellular Location:
    Cell membrane Single-pass type II membrane protein Endoplasmic reticulum membrane Golgi apparatus trans-Golgi network Endosome Lysosome Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
    Protein Attributes:
    Sequence length:
    296
    Sequence:
    50:
    MHRRRSRSCR | EDQKPVMDDQ | RDLISNNEQL | PMLGRRPGAP | ESKCSRGALY | 
    100:
    TGFSILVTLL | LAGQATTAYF | LYQQQGRLDK | LTVTSQNLQL | ENLRMKLPKP | 
    150:
    PKPVSKMRMA | TPLLMQALPM | GALPQGPMQN | ATKYGNMTED | HVMHLLQNAD | 
    200:
    PLKVYPPLKG | SFPENLRHLK | NTMETIDWKV | FESWMHHWLL | FEMSRHSLEQ | 
    250:
    KPTDAPPKVL | TKCQEEVSHI | PAVHPGSFRP | KCDENGNYLP | LQCYGSIGYC | 
    296:
    WCVFPNGTEV | PNTRSRGHHN | CSESLELEDP | SSGLGVTKQD | LGPVPM
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    SMR:
    UniGene:
    Pfam:
    MIM:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human HLA class II histocompatibility antigen gamma chain
    Cat.:
    E13627h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human HLA class II histocompatibility antigen gamma chain
    Cat.:
    E13627r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human HLA class II histocompatibility antigen gamma chain
    Cat.:
    E13627m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human HLA class II histocompatibility antigen gamma chain
    MSDS:
    Please sign in first.
    CLIA Kit for Human HLA class II histocompatibility antigen gamma chain
    Cat.:
    U13627h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human HLA class II histocompatibility antigen gamma chain
    Cat.:
    U13627m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Polyclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Polyclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Monoclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Monoclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Monoclonal Antibody for Human HLA class II histocompatibility antigen gamma chain
    Protein for Human HLA class II histocompatibility antigen gamma chain
    Protein for Human HLA class II histocompatibility antigen gamma chain
    Protein for Human HLA class II histocompatibility antigen gamma chain

    R&D Technical Data

         12% SDS-PAGE Analysis

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 5.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 9.3%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    96

    94-98

    Plasma

    97

    93-99

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    89-99%

    90-101%

    93-101%

    92-106%

    EDTA plasma(n=5)

    89-97%

    90-102%

    89-102%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-108%

    101-109%

    93-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure."
      Claesson L. , Larhammar D. , Rask L. , Peterson P.A.
      Proc. Natl. Acad. Sci. U.S.A.80:7395-7399(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
    2. 2.
      "The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity."
      Strubin M. , Mach B. , Long E.O.
      EMBO J.3:869-872(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
    3. 3.
      "Structure of the human gene encoding the invariant gamma-chain of class II histocompatibility antigens."
      Kudo J. , Chao L.-Y. , Narni F. , Saunders G.F.
      Nucleic Acids Res.13:8827-8841(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 2)
    4. 4.
      "Structure of the human Ia-associated invariant (gamma)-chain gene: identification of 5' sequences shared with major histocompatibility complex class II genes."
      O'Sullivan D.M. , Larhammar D. , Wilson M.C. , Peterson P.A. , Quaranta V.
      Proc. Natl. Acad. Sci. U.S.A.83:4484-4488(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORMS 1 AND 2)
      tissue: Liver.
    5. 5.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
    6. 6.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
      tissue: Heart.
      tissue: Synovium.
    7. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 9.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)
      tissue: B-cell.
      tissue: Tonsil.
    9. 10.
      Bienvenut W.V.
      Submitted (2005-06) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-35; 81-94; 171-179 AND 273-288;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: B-cell lymphoma.
    10. 11.
      "HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides."
      Riberdy J.M. , Newcomb J.R. , Surman M.J. , Barbosa J.A. , Cresswell P.
      Nature360:474-477(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 97-120
    11. 12.
      "Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21)."
      Charest A. , Lane K. , McMahon K. , Park J. , Preisinger E. , Conroy H. , Housman D.
      Genes Chromosomes Cancer37:58-71(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISEASE;CHROMOSOMAL TRANSLOCATION WITH ROS1
    12. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MIF
    13. 14.
      "MHC class II transport at a glance."
      Berger A.C. , Roche P.A.
      J. Cell Sci.122:1-4(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    14. 15.
      "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
      J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-136
      tissue: Liver.
    15. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    16. 17.
      "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
      Halim A. , Nilsson J. , Ruetschi U. , Hesse C. , Larson G.
      Mol. Cell. Proteomics0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-203;STRUCTURE OF CARBOHYDRATES;IDENTIFICATION BY MASS SPECTROMETRY
    17. 18.
      "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A. , Ruetschi U. , Larson G. , Nilsson J.
      J. Proteome Res.12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-203 AND SER-281;IDENTIFICATION BY MASS SPECTROMETRY
    18. 19.
      "The structure of an intermediate in class II MHC maturation: CLIP bound to HLA-DR3."
      Ghosh P. , Amaya M. , Mellins E. , Wiley D.C.
      Nature378:457-462(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 103-117 (CLIP) IN COMPLEX WITH HLA-DRA/HLA-DRB1 HETERODIMER
    19. 20.
      "Structure of a trimeric domain of the MHC class II-associated chaperonin and targeting protein Ii."
      Jasanoff A. , Wagner G. , Wiley D.C.
      EMBO J.17:6812-6818(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 134-208
    20. 21.
      "Crystal structure of MHC class II-associated p41 Ii fragment bound to cathepsin L reveals the structural basis for differentiation between cathepsins L and S."
      Guncar G. , Pungercic G. , Klemencic I. , Turk V. , Turk D.
      EMBO J.18:793-803(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 210-274;GLYCOSYLATION AT ASN-256;DISULFIDE BONDS
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