Hsf1 ELISA Kit for Mouse(Mus musculus) | EIAab.comHsf1 ELISA Kit for Mouse(Mus musculus)

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Hsf1 (Gene name),

Heat shock factor protein 1 (Protein name ), HSF1_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Hsf1;

Protein name:

Heat shock factor protein 1(HSF 1);

Alternative:

Heat shock transcription factor 1(HSTF 1);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Monomer (By similarity). Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.

Function:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Subcellular Location:

Cytoplasm Nucleus Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.

Protein Attributes:

Sequence length:

525

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

525:

DYTDDPTISL | LTGTEPHKAK | DPTVS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P38532

UniGene:

Mm.347444

Pfam:

PF00447

SMR:

P38532

STRING:

10090.ENSMUSP00000023215

KEGG:

mmu:15499

Uniprot:

P38532

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ELISA Kit for Mouse HSF 1

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E0435h

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96T

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ELISA Kit for Mouse HSF 1

Cat.:

E0435m

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96T

ELISA Kit for Mouse HSF 1

Cat.:

E0435b

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96T

ELISA Kit for Mouse HSF 1

Cat.:

E0435c

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96T

CLIA Kit for Mouse HSF 1

Cat.:

U0435b

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96T

CLIA Kit for Mouse HSF 1

Cat.:

U0435h

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96T

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CLIA Kit for Mouse HSF 1

Cat.:

U0435c

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96T

CLIA Kit for Mouse HSF 1

Cat.:

U0435m

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96T

Polyclonal Antibody for Mouse HSF 1

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P0435Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse HSF 1

Cat.:

P0435Rb-h

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40ug/0.2ml

Monoclonal Antibody for Mouse HSF 1

Protein for Mouse HSF 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of HSF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	90-99
Plasma	98	96-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-100%	91-104%	95-99%	92-102%
EDTA plasma(n=5)	90-103%	94-100%	94-102%	91-105%
heparin plasma(n=5)	96-99%	92-102%	90-106%	93-100%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability."
Sarge K.D. , Zimarino V. , Holm K. , Wu C. , Morimoto R.I.
Genes Dev.5:1902-1911(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
strain: WEHI-3.
2.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
3.

"Structural organization and promoter analysis of murine heat shock transcription factor-1 gene."
Zhang Y. , Koushik S. , Dai R. , Mivechi N.F.
J. Biol. Chem.273:32514-32521(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-525 (ISOFORM 2)
strain: 129/SvJ.
tissue: Liver.
4.

"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M. , Bailey C.M. , Cunningham D.L. , Heath J.K. , Cooper H.J.
Mol. Cell. Proteomics8:904-912(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303 AND SER-307;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic fibroblast.

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