IDE ELISA Kit for Human(Homo sapiens) | EIAab.comIDE ELISA Kit for Human(Homo sapiens)

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IDE (Gene name),

Insulin-degrading enzyme (Protein name ), IDE_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IDE;

Protein name:

Insulin-degrading enzyme;

Alternative:

Insulin protease(Insulinase);Abeta-degrading protease;Insulysin;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.

Function:

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Subcellular Location:

Cytoplasm Cell surface Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

Protein Attributes:

Sequence length:

1019

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

1000:

1019:

GLPLFPLVKP | HINFMAAKL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:3416

Pfam:

PF00675

UniGene:

Hs.500546

String:

P14735

SMR:

P14735

MIM:

146680

STRING:

9606.ENSP00000265986

Uniprot:

P14735

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ELISA Kit for Human Insulin-degrading enzyme

Cat.:

E1897m

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96T

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ELISA Kit for Human Insulin-degrading enzyme

Cat.:

E1897h

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96T

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ELISA Kit for Human Insulin-degrading enzyme

Cat.:

E1897r

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ELISA Kit for Human Insulin-degrading enzyme

Cat.:

E1897b

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Packing:

96T

CLIA Kit for Human Insulin-degrading enzyme

Cat.:

U1897b

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MSDS:

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Packing:

96T

CLIA Kit for Human Insulin-degrading enzyme

Cat.:

U1897r

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MSDS:

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Packing:

96T

CLIA Kit for Human Insulin-degrading enzyme

Cat.:

U1897h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human Insulin-degrading enzyme

Cat.:

U1897m

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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Polyclonal Antibody for Human Insulin-degrading enzyme

Cat.:

P1897Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Insulin-degrading enzyme

Cat.:

P1897Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Insulin-degrading enzyme

Cat.:

P1897Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Insulin-degrading enzyme

Monoclonal Antibody for Human Insulin-degrading enzyme

Protein for Human Insulin-degrading enzyme

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III."
Affholter J.A. , Fried V.A. , Roth R.A.
Science242:1415-1418(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PARTIAL PROTEIN SEQUENCE
2.

"Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes."
Affholter J.A. , Hsieh C.L. , Francke U. , Roth R.A.
Mol. Endocrinol.4:1125-1135(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);SEQUENCE REVISION
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
tissue: Testis.
4.

"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P. , Earthrowl M.E. , Grafham D.V. , Rubenfield M. , French L. , Steward C.A. , Sims S.K. , Jones M.C. , Searle S. , Scott C. , Howe K. , Hunt S.E. , Andrews T.D. , Gilbert J.G.R. , Swarbreck D. , Ashurst J.L. , Taylor A. , Battles J. , more...
Nature429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
7.

"Neurons regulate extracellular levels of amyloid beta-protein via proteolysis by insulin-degrading enzyme."
Vekrellis K. , Ye Z. , Qiu W.Q. , Walsh D. , Hartley D. , Chesneau V. , Rosner M.R. , Selkoe D.J.
J. Neurosci.20:1657-1665(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
8.

"Insulin degrading enzyme is a cellular receptor mediating varicella-zoster virus infection and cell-to-cell spread."
Li Q. , Ali M.A. , Cohen J.I.
Cell127:305-316(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH VZV GLYCOPROTEIN E
9.

"The amino terminus of varicella-zoster virus (VZV) glycoprotein E is required for binding to insulin-degrading enzyme, a VZV receptor."
Li Q. , Krogmann T. , Ali M.A. , Tang W.-J. , Cohen J.I.
J. Virol.81:8525-8532(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH VZV GLYCOPROTEIN E
10.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
11.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
12.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
13.

"Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism."
Shen Y. , Joachimiak A. , Rosner M.R. , Tang W.-J.
Nature443:870-874(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 42-1019 OF MUTANT GLN-111 IN COMPLEXES WITH ZINC IONS; IAPP; INSULIN; AMYLOID AND GLUCAGON;MUTAGENESIS OF GLU-111; SER-132; ASN-184; ASP-426; GLU-817; GLN-828 AND LYS-899;COFACTOR;SUBUNIT;ACTIVE SITE
14.

"Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE."
Im H. , Manolopoulou M. , Malito E. , Shen Y. , Zhao J. , Neant-Fery M. , Sun C.-Y. , Meredith S.C. , Sisodia S.S. , Leissring M.A. , Tang W.-J.
J. Biol. Chem.282:25453-25463(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 43-1018 OF MUTANT PHE-831 IN COMPLEX WITH ZINC IONS AND SUBSTRATE PEPTIDE;CATALYTIC ACTIVITY;ENZYME REGULATION;ATP-BINDING;SUBUNIT;MUTAGENESIS OF ASP-426 AND LYS-899;FUNCTION
15.

"Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme."
Malito E. , Ralat L.A. , Manolopoulou M. , Tsay J.L. , Wadlington N.L. , Tang W.-J.
Biochemistry47:12822-12834(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 42-1019 OF MUTANT GLN-111 IN COMPLEX WITH BRADYKININ;FUNCTION;ENZYME REGULATION

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