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Index > Protein center > IGF2(Gene name) > Human
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  • IGF2 (Gene name),
  • Insulin-like growth factor II (Protein name ),  IGF2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Vaild Sequence
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  • Gene name:
    IGF2(PP1446);
    Protein name:
    Insulin-like growth factor II(IGF-II);
    Alternative:
    Preptin;Somatomedin-A;
    Organism:
    IGF2 (Homo sapiens). 
    General Annotation
    Sub Unit:
    Function:
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    180
    Sequence:
    50:
    MGIPMGKSML | VLLTFLAFAS | CCIAAYRPSE | TLCGGELVDT | LQFVCGDRGF | 
    100:
    YFSRPASRVS | RRSRGIVEEC | CFRSCDLALL | ETYCATPAKS | ERDVSTPPTV | 
    150:
    LPDNFPRYPV | GKFFQYDTWK | QSTQRLRRGL | PALLRARRGH | VLAKELEAFR | 
    180:
    EAKRHRPLIA | LPTQDPAHGG | APPEMASNRK | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    MIM:
    KEGG:
    UniGene:
    SMR:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human IGF-II
    Cat.:
    E0051r
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    96T
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    ELISA Kit for Human IGF-II
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    E0051p
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    ELISA Kit for Human IGF-II
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    E0051m
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    ELISA Kit for Human IGF-II
    Cat.:
    E0051h
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    96T
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    ELISA Kit for Human IGF-II
    Cat.:
    E0051c
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human IGF-II
    Cat.:
    E0051b
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    MSDS:
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    96T
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    CLIA Kit for Human IGF-II
    Cat.:
    U0051b
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    96T
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    CLIA Kit for Human IGF-II
    Cat.:
    U0051c
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human IGF-II
    Cat.:
    U0051h
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    MSDS:
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    96T
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    CLIA Kit for Human IGF-II
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    U0051m
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    CLIA Kit for Human IGF-II
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    96T
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    CLIA Kit for Human IGF-II
    Cat.:
    U0051r
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    96T
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    Polyclonal Antibody for Human IGF-II
    Cat.:
    P0051Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IGF-II
    Polyclonal Antibody for Human IGF-II
    Cat.:
    P0051Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IGF-II
    Polyclonal Antibody for Human IGF-II
    Cat.:
    P0051Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Monoclonal Antibody for Human IGF-II
    Protein for Human IGF-II
    Protein for Human IGF-II
    Protein for Human IGF-II
    Protein for Human IGF-II
    Protein for Human IGF-II
    Protein for Human IGF-II

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.7%

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    Recovery
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    Recovery was determined by spiking various levels of IGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    87-95

    Plasma

    92

    90-97

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-96%

    85-92%

    87-98%

    91-97%

    EDTA plasma(n=5)

    93-95%

    88-96%

    95-100%

    86-93%

    heparin plasma(n=5)

    88-94%

     

    91-98%

    100-106%

    80-87

     

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    References
    1. 1.
      "Insulin-like growth factor II precursor gene organization in relation to insulin gene family."
      Dull T.J. , Gray A. , Hayflick J.S. , Ullrich A.
      Nature310:777-781(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
    2. 2.
      "Sequence of a cDNA clone encoding human preproinsulin-like growth factor II."
      Bell G.I. , Merryweather J.P. , Sanchez-Pescador R. , Stempien M.M. , Priestley L. , Scott J. , Rall L.B.
      Nature310:775-777(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    3. 3.
      "Isolation of an insulin-like growth factor II cDNA with a unique 5' untranslated region from human placenta."
      Shen S.-J. , Daimon M. , Wang C.-Y. , Jansen M. , Ilan J.
      Proc. Natl. Acad. Sci. U.S.A.85:1947-1951(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    4. 4.
      "Organization of the human genes for insulin-like growth factors I and II."
      de Pagter-Holthuizen P. , van Schaik F.M.A. , Verduijn G.M. , van Ommen G.J.B. , Bouma B.N. , Jansen M. , Sussenbach J.S.
      FEBS Lett.195:179-184(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
    5. 5.
      "Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions."
      Irminger J.C. , Rosen K.M. , Humbel R.E. , Villa-Komaroff L.
      Proc. Natl. Acad. Sci. U.S.A.84:6330-6334(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    6. 6.
      "Human insulin-like growth factor I and II messenger RNA: isolation of complementary DNA and analysis of expression."
      Rall L.B. , Scott J. , Bell G.I.
      Methods Enzymol.146:239-248(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    7. 7.
      "Nucleotide sequences of cDNAs encoding precursors of human insulin-like growth factor II (IGF-II) and an IGF-II variant."
      Jansen M. , van Schaik F.M.A. , van Tol H. , van den Brande J.L. , Sussenbach J.S.
      FEBS Lett.179:243-246(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)
    8. 8.
      "Isolation of a cDNA for a growth factor of vascular endothelial cells from human lung cancer cells: its identity with insulin-like growth factor II."
      Hagiwara K. , Kobayashi T. , Tobita M. , Kikyo N. , Yazaki Y. , Okabe T.
      Jpn. J. Cancer Res.86:202-207(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    9. 9.
      "Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human."
      Monk D. , Sanches R. , Arnaud P. , Apostolidou S. , Hills F.A. , Abu-Amero S. , Murrell A. , Friess H. , Reik W. , Stanier P. , Constancia M. , Moore G.E.
      Hum. Mol. Genet.15:1259-1269(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    10. 10.
      "Down-regulation of achaete-scute complex homolog 1 (ASCL1) in neuroblastoma cells induces up-regulation of insulin-like growth factor 2 (IGF2)."
      Li J. , Neumann I. , Volkmer I. , Staege M.S.
      Mol. Biol. Rep.38:1515-1521(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-165 (ISOFORM 3);ALTERNATIVE SPLICING
    11. 11.
      "Large-scale cDNA transfection screening for genes related to cancer development and progression."
      Wan D. , Gong Y. , Qin W. , Zhang P. , Li J. , Wei L. , Zhou X. , Li H. , Qiu X. , Zhong F. , He L. , Yu J. , Yao G. , Jiang H. , Qian L. , Yu Y. , Shu H. , Chen X. , more...
      Proc. Natl. Acad. Sci. U.S.A.101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    12. 12.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    13. 13.
      SeattleSNPs variation discovery resource
      Submitted (2002-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    14. 14.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Cerebellum.
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    17. 17.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Muscle.
    18. 18.
      "Differential expression of the human insulin-like growth factor II gene. Characterization of the IGF-II mRNAs and an mRNA encoding a putative IGF-II-associated protein."
      de Pagter-Holthuizen P. , van der Kammen R.A. , Jansen M. , van Schaik F.M.A. , Sussenbach J.S.
      Biochim. Biophys. Acta950:282-295(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-180
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) OF 25-91 IN COMPLEX WITH IGF2R;DISULFIDE BONDS
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