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Index > Protein center > Il27(Gene name) > Mouse
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  • Il27 (Gene name),
  • Interleukin-27 subunit alpha (Protein name ),  IL27A_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Il27(Il27a);
    Protein name:
    Interleukin-27 subunit alpha(IL-27 subunit alpha;IL-27-A;IL27-A);
    Alternative:
    p28;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Heterodimer with EBI3. IL-27 is an heterodimer composed IL-27 subunit alpha and EBI3. EBI3 is also a component of the IL-12 heterodimer.
    Function:
    Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.
    Subcellular Location:
    Secreted Poorly secreted without coexpression of EBI3.
    Protein Attributes:
    Sequence length:
    234
    Sequence:
    50:
    MGQVTGDLGW | RLSLLLLPLL | LVQAGSWGFP | TDPLSLQELR | REFTVSLYLA | 
    100:
    RKLLSEVQGY | VHSFAESRLP | GVNLDLLPLG | YHLPNVSLTF | QAWHHLSDSE | 
    150:
    RLCFLATTLR | PFPAMLGGLG | TQGTWTSSER | EQLWAMRLDL | RDLHRHLRFQ | 
    200:
    VLAAGFKCSK | EEEDKEEEEE | EEEEEKKLPL | GALGGPNQVS | SQVSWPQLLY | 
    234:
    TYQLLHSLEL | VLSRAVRDLL | LLSLPRRPGS | AWDS
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse IL-27 subunit alpha
    Cat.:
    E0385h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse IL-27 subunit alpha
    Cat.:
    E0385m
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Mouse IL-27 subunit alpha
    Cat.:
    E0385p
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse IL-27 subunit alpha
    Cat.:
    U0385h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse IL-27 subunit alpha
    Cat.:
    U0385p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse IL-27 subunit alpha
    Cat.:
    U0385m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Mouse IL-27 subunit alpha
    Polyclonal Antibody for Mouse IL-27 subunit alpha
    Cat.:
    P0385Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse IL-27 subunit alpha
    Cat.:
    P0385Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse IL-27 subunit alpha
    Monoclonal Antibody for Mouse IL-27 subunit alpha
    Monoclonal Antibody for Mouse IL-27 subunit alpha
    Protein for Mouse IL-27 subunit alpha
    Protein for Mouse IL-27 subunit alpha
    Protein for Mouse IL-27 subunit alpha

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.8%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of IL27A into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    104

    100-108

    Plasma

    103

    98-105

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL27A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    100-106%

    98-106%

    95-105%

    103-109%

    EDTA plasma(n=5)

    95-100%

    102-107%

    97-104%

    102-108%

    heparin plasma(n=5)

    98-105%

     

    106-110%

    100-108%

    96-108%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Citations
    [2].
    M.L.P. Van Der Hoorn, R. Keijser, C. Ris-Stalpers, G. Afink, F.H.J. Claas, J.A.M. Van Der Post, S.A. Scherjon
    References
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;SUBUNIT
      strain: NZB.
    2. 2.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Bone marrow.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Brain.
    4. 4.
      "IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells."
      Hibbert L. , Pflanz S. , De Waal Malefyt R. , Kastelein R.A.
      J. Interferon Cytokine Res.23:513-522(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    5. 5.
      "IL-27 regulates IL-12 responsiveness of naive CD4+ T cells through Stat1-dependent and -independent mechanisms."
      Lucas S. , Ghilardi N. , Li J. , de Sauvage F.J.
      Proc. Natl. Acad. Sci. U.S.A.100:15047-15052(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    7. 7.
      "IL-27 mediates complete regression of orthotopic primary and metastatic murine neuroblastoma tumors: role for CD8+ T cells."
      Salcedo R. , Stauffer J.K. , Lincoln E. , Back T.C. , Hixon J.A. , Hahn C. , Shafer-Weaver K. , Malyguine A. , Kastelein R. , Wigginton J.M.
      J. Immunol.173:7170-7182(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    8. 8.
      "Production of IL-27 and other IL-12 family cytokines by microglia and their subpopulations."
      Sonobe Y. , Yawata I. , Kawanokuchi J. , Takeuchi H. , Mizuno T. , Suzumura A.
      Brain Res.1040:202-207(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
    9. 9.
      "Expression of IL-27 in murine carcinoma cells produces antitumor effects and induces protective immunity in inoculated host animals."
      Chiyo M. , Shimozato O. , Yu L. , Kawamura K. , Iizasa T. , Fujisawa T. , Tagawa M.
      Int. J. Cancer115:437-442(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    10. 10.
      "Augmentation of effector CD8+ T cell generation with enhanced granzyme B expression by IL-27."
      Morishima N. , Owaki T. , Asakawa M. , Kamiya S. , Mizuguchi J. , Yoshimoto T.
      J. Immunol.175:1686-1693(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    11. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    12. 12.
      "IL-27 suppresses CD28-mediated IL-2 production through suppressor of cytokine signaling 3."
      Owaki T. , Asakawa M. , Kamiya S. , Takeda K. , Fukai F. , Mizuguchi J. , Yoshimoto T.
      J. Immunol.176:2773-2780(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    13. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    14. 14.
      "Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism."
      Yoshimura T. , Takeda A. , Hamano S. , Miyazaki Y. , Kinjyo I. , Ishibashi T. , Yoshimura A. , Yoshida H.
      J. Immunol.177:5377-5385(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    15. 15.
      "IL-27 induces Th1 differentiation via p38 MAPK/T-bet- and intercellular adhesion molecule-1/LFA-1/ERK1/2-dependent pathways."
      Owaki T. , Asakawa M. , Fukai F. , Mizuguchi J. , Yoshimoto T.
      J. Immunol.177:7579-7587(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    16. 16.
      "Interleukin 27 limits autoimmune encephalomyelitis by suppressing the development of interleukin 17-producing T cells."
      Batten M. , Li J. , Yi S. , Kljavin N.M. , Danilenko D.M. , Lucas S. , Lee J. , de Sauvage F.J. , Ghilardi N.
      Nat. Immunol.7:929-936(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MOUSE MODEL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALITIS;FUNCTION
    17. 17.
      "Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system."
      Stumhofer J.S. , Laurence A. , Wilson E.H. , Huang E. , Tato C.M. , Johnson L.M. , Villarino A.V. , Huang Q. , Yoshimura A. , Sehy D. , Saris C.J.M. , O'Shea J.J. , Hennighausen L. , Ernst M. , Hunter C.A.
      Nat. Immunol.7:937-945(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MOUSE MODEL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALITIS;FUNCTION;INDUCTION
    18. 18.
      "IL-27 controls the development of inducible regulatory T cells and Th17 cells via differential effects on STAT1."
      Neufert C. , Becker C. , Wirtz S. , Fantini M.C. , Weigmann B. , Galle P.R. , Neurath M.F.
      Eur. J. Immunol.37:1809-1816(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    19. 19.
      "IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3."
      Molle C. , Nguyen M. , Flamand V. , Renneson J. , Trottein F. , De Wit D. , Willems F. , Goldman M. , Goriely S.
      J. Immunol.178:7607-7615(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
    20. 20.
      "Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis."
      Fitzgerald D.C. , Ciric B. , Touil T. , Harle H. , Grammatikopolou J. , Das Sarma J. , Gran B. , Zhang G.-X. , Rostami A.
      J. Immunol.179:3268-3275(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MOUSE MODEL OF EXPERIMENTAL AUTOIMMUNE ENCEPHALITIS;INDUCTION
    21. 21.
      "The biology and therapeutic potential of interleukin 27."
      Batten M. , Ghilardi N.
      J. Mol. Med.85:661-672(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    22. 22.
      "Peroxisome proliferator-activated receptor-alpha agonist fenofibrate regulates IL-12 family cytokine expression in the CNS: relevance to multiple sclerosis."
      Xu J. , Racke M.K. , Drew P.D.
      J. Neurochem.103:1801-1810(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
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