IL6 ELISA Kit for Human(Homo sapiens) | EIAab.comIL6 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > IL6(Gene name) > Human

IL6 (Gene name),

Interleukin-6 (Protein name ), IL6_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (4)

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Gene name:

IL6(IFNB2);

Protein name:

Interleukin-6(IL-6);

Alternative:

CTL differentiation factor(CDF);B-cell stimulatory factor 2(BSF-2);Interferon beta-2(IFN-beta-2);Hybridoma growth factor;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

212

Sequence:

50:

100:

150:

200:

212:

FLQSSLRALR | QM

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000258743

SMR:

P05231

MIM:

147620

Pfam:

PF00489

UniGene:

Hs.654458

String:

P05231

KEGG:

hsa:3569

Uniprot:

P05231

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ELISA Kit for Human IL-6

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E0079p

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96T

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ELISA Kit for Human IL-6

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E0079d

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96T

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ELISA Kit for Human IL-6

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E0079r

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ELISA Kit for Human IL-6

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E0079b

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96T

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ELISA Kit for Human IL-6

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E0079c

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96T

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ELISA Kit for Human IL-6

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E0079h

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96T

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ELISA Kit for Human IL-6

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E0079m

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ELISA Kit for Human IL-6

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CLIA Kit for Human IL-6

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CLIA Kit for Human IL-6

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U0079r

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96T

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CLIA Kit for Human IL-6

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U0079m

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96T

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CLIA Kit for Human IL-6

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96T

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CLIA Kit for Human IL-6

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U0079h

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96T

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CLIA Kit for Human IL-6

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U0079b

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CLIA Kit for Human IL-6

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U0079d

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CLIA Kit for Human IL-6

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U0079c

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Polyclonal Antibody for Human IL-6

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P0079Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human IL-6

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P0079Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human IL-6

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P0079Rb-m

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40ug/0.2ml

Monoclonal Antibody for Human IL-6

Protein for Human IL-6

Cat.:

R0079h

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Packing:

1mg

Purity:

>90%

Protein for Human IL-6

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-102
Plasma	98	94-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	90	85-96
Plasma	91	87-93

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	93	86-99
Plasma	95	86-101

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	91	87-94
Plasma	92	89-93

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	90-99%	89-97%	96-102%
EDTA plasma(n=5)	87-95%	92-98%	100-105%	97-106%
heparin plasma(n=5)	91-100%	94-103%	105-108%	91-97%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-93%	92-90%	86-91%	89-94%
EDTA plasma(n=5)	82-86%	86-92%	87-93%	90-96%
heparin plasma(n=5)	91-94%	82-86%	81-93%	84-95%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-101%	93-102%	97-108%	94-110%
EDTA plasma(n=5)	101-105%	97-109%	88-97%	90-105%
heparin plasma(n=5)	96-107%	98-110%	100-111%	90-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-96%	90-93%	85-89%	82-87%
EDTA plasma(n=5)	89-95%	92-96%	85-87%	90-95%
heparin plasma(n=5)	86-93%	85-92%	83-87%	91-96%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Effect of high-dose rosuvastatin loading before percutaneous coronary intervention in female patients with non-ST-segment elevation acute coronary..."

Y Gao, ZM Jia, YJ Sun, et al.

From:Chin Med J (Engl)

[2].

"Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice"

Xiao Yu, Ning Shen, Ming-Liang Zhang,, Fei-Yan Pan, Chen Wang, Wei-Ping Jia, Chang Liu, Qian Gao, Xiang Gao, Bin Xue and Chao-Jun Li

From:The EMBO Journal

[3].

"Preclinical efficacy of Dexmedetomidine on spinal cord injury provoked oxidative renal damage"

Shou-Shi W, Ting-Ting S, Ji-Shun N, et al.

From:Renal failure

[4].

"Promoter methylation status and expression of PPAR-γ gene are associated with prognosis of acute-on-chronic hepatitis B liver failure"

Zhao Z H, Fan Y C, Zhao Q, et al.

From:Clinical epigenetics

References

1.

"Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin."
Hirano T. , Yasukawa K. , Harada H. , Taga T. , Watanabe Y. , Matsuda T. , Kashiwamura S. , Nakajima K. , Koyama K. , Iwamatsu A. , Tsunasawa S. , Sakiyama F. , Matsui H. , Takahara Y. , Taniguchi T. , Kishimoto T.
Nature324:73-76(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
2.

"Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene."
Yasukawa K. , Hirano T. , Watanabe Y. , Muratani K. , Matsuda T. , Nakai S. , Kishimoto T.
EMBO J.6:2939-2945(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Anti-beta-interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: structural studies of the beta 2 interferon involved."
May L.T. , Helfgott D.C. , Sehgal P.B.
Proc. Natl. Acad. Sci. U.S.A.83:8957-8961(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines."
Zilberstein A. , Ruggieri R. , Korn J.H. , Revel M.
EMBO J.5:2529-2537(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Molecular cloning and expression of hybridoma growth factor in Escherichia coli."
Brakenhoff J.P.J. , de Groot E.R. , Evers R.F. , Pannekoek H. , Aarden L.A.
J. Immunol.139:4116-4121(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.

"Deletion of 3' untranslated region of human BSF-2 mRNA causes stabilization of the mRNA and high-level expression in mouse NIH3T3 cells."
Tonouchi N. , Miwa K. , Karasuyama H. , Matsui H.
Biochem. Biophys. Res. Commun.163:1056-1062(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
7.

"Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts."
Haegeman G. , Content J. , Volckaert G. , Derynck R. , Tavernier J. , Fiers W.
Eur. J. Biochem.159:625-632(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
tissue: Fibroblast.
8.

"Interleukin 6: identification as a hematopoietic colony-stimulating factor."
Wong G. , Witek-Giannotti J. , Hewick R. , Clark S. , Ogawa M.
Behring Inst. Mitt.83:40-47(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
9.

"Stable and efficient expression of human interleukin-6 cDNA in mammalian cells after gene transfer."
Chen Q.Y.
Zhonghua Zhong Liu Za Zhi14:340-344(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
10.

SeattleSNPs variation discovery resource
Submitted (2001-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS SER-32 AND VAL-162
11.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
12.

"Separation and comparison of two monokines with lymphocyte-activating factor activity: IL-1 beta and hybridoma growth factor (HGF). Identification of leukocyte-derived HGF as IL-6."
van Damme J. , van Beeumen J. , Decock B. , van Snick J. , de Ley M. , Billiau A.
J. Immunol.140:1534-1541(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 30-63
13.

"Interleukin 6 is the principal cytolytic T lymphocyte differentiation factor for thymocytes in human leukocyte conditioned medium."
Ming J.E. , Cernetti C. , Steinman R.M. , Granelli-Piperno A.
J. Mol. Cell. Immunol.4:203-211(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 30-50
14.

"Marked cell-type-specific differences in glycosylation of human interleukin-6."
May L.T. , Shaw J.E. , Khanna A.K. , Zabriskie J.B. , Sehgal P.B.
Cytokine3:204-211(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 30-40;GLYCOSYLATION
15.

"Structure, stability and biological properties of a N-terminally truncated form of recombinant human interleukin-6 containing a single disulfide bond."
Breton J. , la Fiura A. , Bertolero F. , Orsini G. , Valsasina B. , Ziliotto R. , de Filippis V. , Polverino de Laureto P. , Fontana A.
Eur. J. Biochem.227:573-581(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 50-212 OF RECOMBINANT FORM LACKING FIRST DISULFIDE BOND
16.

"Disulfide structures of human interleukin-6 are similar to those of human granulocyte colony stimulating factor."
Clogston C.L. , Boone T.C. , Crandall B.C. , Mendiaz E.A. , Lu H.S.
Arch. Biochem. Biophys.272:144-151(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
17.

"Evidence for the importance of a positive charge and an alpha-helical structure of the C-terminus for biological activity of human IL-6."
Luetticken C. , Kruettgen A. , Moeller C. , Heinrich P.C. , Rose-John S.
FEBS Lett.282:265-267(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS
18.

"Folding topologies of human interleukin-6 and its mutants as studied by NMR spectroscopy."
Nishimura C. , Watanabe A. , Gouda H. , Shimada I. , Arata Y.
Biochemistry35:273-281(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
19.

"Solution structure of recombinant human interleukin-6."
Xu G.-Y. , Yu H.-A. , Hong J. , Stahl M. , McDonagh T. , Kay L.E. , Cumming D.A.
J. Mol. Biol.268:468-481(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
20.

"1.9-A crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling."
Somers W. , Stahl M. , Seehra J.S.
EMBO J.16:989-997(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
21.

"The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis."
Fishman D. , Faulds G. , Jeffery R. , Mohamed-Ali V. , Yudkin J.S. , Humphries S. , Woo P.
J. Clin. Invest.102:1369-1376(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO SYSTEMIC JUVENILE RHEUMATOID ARTHRITIS
22.

"An IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in men infected with human immunodeficiency virus."
Foster C.B. , Lehrnbecher T. , Samuels S. , Stein S. , Mol F. , Metcalf J.A. , Wyvill K. , Steinberg S.M. , Kovacs J. , Blauvelt A. , Yarchoan R. , Chanock S.J.
Blood96:2562-2567(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO KAPOSI SARCOMA
23.

"A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density."
Ota N. , Nakajima T. , Nakazawa I. , Suzuki T. , Hosoi T. , Orimo H. , Inoue S. , Shirai Y. , Emi M.
J. Hum. Genet.46:267-272(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN BMD
24.

"Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women."
Chung H.W. , Seo J.-S. , Hur S.E. , Kim H.L. , Kim J.Y. , Jung J.H. , Kim L.H. , Park B.L. , Shin H.D.
J. Hum. Genet.48:243-248(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN BMD

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