Il6 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comIl6 ELISA Kit for Rat(Rattus norvegicus)

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Index > Protein center > Il6(Gene name) > Rat

Il6 (Gene name),

Interleukin-6 (Protein name ), IL6_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (9)

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Gene name:

Il6(Il-6);

Protein name:

Interleukin-6(IL-6);

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

211

Sequence:

50:

100:

150:

200:

211:

LKVTMRSTRQ | T

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P20607

UniGene:

Rn.9873

Pfam:

PF00489

STRING:

10116.ENSRNOP00000013732

KEGG:

rno:24498

Uniprot:

P20607

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ELISA Kit for Rat IL-6

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E0079p

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079d

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079r

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079b

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079c

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079h

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96T

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ELISA Kit for Rat IL-6

Cat.:

E0079m

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96T

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ELISA Kit for Rat IL-6

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CLIA Kit for Rat IL-6

MSDS:

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CLIA Kit for Rat IL-6

Cat.:

U0079r

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

Cat.:

U0079m

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

Cat.:

U0079p

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

Cat.:

U0079h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

Cat.:

U0079b

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

Cat.:

U0079d

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat IL-6

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U0079c

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96T

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Polyclonal Antibody for Rat IL-6

Cat.:

P0079Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat IL-6

Cat.:

P0079Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat IL-6

Cat.:

P0079Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat IL-6

Protein for Rat IL-6

Cat.:

R0079h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Rat IL-6

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-102
Plasma	98	94-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	90	85-96
Plasma	91	87-93

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	93	86-99
Plasma	95	86-101

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of IL-6 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	91	87-94
Plasma	92	89-93

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	90-99%	89-97%	96-102%
EDTA plasma(n=5)	87-95%	92-98%	100-105%	97-106%
heparin plasma(n=5)	91-100%	94-103%	105-108%	91-97%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-93%	92-90%	86-91%	89-94%
EDTA plasma(n=5)	82-86%	86-92%	87-93%	90-96%
heparin plasma(n=5)	91-94%	82-86%	81-93%	84-95%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-101%	93-102%	97-108%	94-110%
EDTA plasma(n=5)	101-105%	97-109%	88-97%	90-105%
heparin plasma(n=5)	96-107%	98-110%	100-111%	90-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-96%	90-93%	85-89%	82-87%
EDTA plasma(n=5)	89-95%	92-96%	85-87%	90-95%
heparin plasma(n=5)	86-93%	85-92%	83-87%	91-96%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Molecular mechanisms of neuroprotective effect of adjuvant therapy with phenytoin in pentylenetetrazole-induced seizures: Impact on Sirt1/NRF2 signaling pathways"

M M. Nagib, M G. Tadros, R M. Rahmo, et al.

From:NeuroToxicology

[2].

"Effect of ω-3 Fatty Acid on GastrointestinalMotility after Abdominal Operation in Rats"

Q Zhang, JC Yu, WM Kang, et al.

From:Mediators of Inflammation

[3].

"Alleviating effects of morin against experimentally-induced diabetic osteopenia"

Hatem M Abuohashish,Salim S Al-Rejaie ,Khaled A Al-Hosaini,Mihir Y Parmar,Mohammed M Ahmed

From:Diabetology & Metabolic Syndrome

[4].

"Cerebroprotective potential of resveratrol through anti-oxidant and anti-inflammatory mechanisms in rats"

Prabhakar Orsu , B. V. S. N. Murthy and Annapurna Akula

From:Journal of Neural Transmission

[5].

"Flavonoid, morin inhibits oxidative stress, inflammation and enhances neurotrophic support in the brain of streptozotocin-induced diabetic rats"

MS Ola, AM Aleisa, SS Al-Rejaie, et al.

From:Neurological Sciences

[6].

"Transgenic flax overexpressing polyphenols as a potential anti-inflammatory dietary agent"

M Matusiewicz, I Kosieradzka, M Sobczak-Filipiak, et al.

From:Journal of Functional Foods

[7].

"Efficacy of coenzyme Q10 in mitigating spinal cord injury‑induced osteoporosis"

Zhang X X, Qian K J, Zhang Y, et al.

From:Molecular medicine reports

[8].

"Paeoniflorin ameliorates acute myocardial infarction of rats by inhibiting inflammation and inducible nitric oxide synthase signaling pathways"

Chen C, Du P, Wang J.

From:Molecular medicine reports

[9].

"Therapeutic effectiveness of rectally administered fish oil and mesalazine in trinitrobenzenesulfonic acid-induced colitis"

E Yorulmaz, H Yorulmaz, O Zengi, et al.

From:Biomedicine & Pharmacotherapy

References

1.

"Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells."
Northemann W. , Braciak T.A. , Hattori M. , Lee F. , Fey G.H.
J. Biol. Chem.264:16072-16082(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]

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