IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
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12% SDS-PAGE Analysis
s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 6.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 8.6%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
Recovery was determined by spiking various levels of IL8 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
100
97-106
Plasma
101
95-104
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
93-105
Plasma
98
94-106
Recovery was determined by spiking various levels of IL8 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
105
100-106
Plasma
104
102-107
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-105%
97-104%
95-101%
103-106%
EDTA plasma(n=5)
98-104%
94-103%
90-97%
105-109%
heparin plasma(n=5)
94-99%
103-108%
96-104%
98-105%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
91-103%
92-105%
95-106%
94-109%
EDTA plasma(n=5)
89-95%
93-103%
94-102%
95-106%
heparin plasma(n=5)
93-105%
94-107%
101-106%
96-108%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
98-102%
103-107%
97-105%
96-103%
EDTA plasma(n=5)
100-104%
99-107%
102-108%
97-102%
heparin plasma(n=5)
104-106%
94-102%
108-112%
97-103%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 26-45