KRT2 ELISA Kit for Human(Homo sapiens) | EIAab.comKRT2 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > KRT2(Gene name) > Human

KRT2 (Gene name),

Keratin, type II cytoskeletal 2 epidermal (Protein name ), K22E_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

KRT2(KRT2A;KRT2E);

Protein name:

Keratin, type II cytoskeletal 2 epidermal;

Alternative:

Epithelial keratin-2e;Cytokeratin-2e(CK-2e);Keratin-2e(K2e);Keratin-2 epidermis;Type-II keratin Kb2;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterotetramer of two type I and two type II keratins. Associates with KRT10.

Function:

Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

639

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

639:

GSRGGSSSGG | GYGSGGGGSS | SVKGSSGEAF | GSSVTFSFR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

146800

Pfam:

PF00038

String:

P35908

KEGG:

hsa:3849

SMR:

P35908

STRING:

9606.ENSP00000310861

UniGene:

Hs.707

Uniprot:

P35908

FOR

ELISA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

E1427h

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96T

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ELISA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

E1427d

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96T

ELISA Kit for Human Keratin, type II cytoskeletal 2 epidermal

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E1427m

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96T

ELISA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

E1427r

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96T

CLIA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

U1427r

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96T

CLIA Kit for Human Keratin, type II cytoskeletal 2 epidermal

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U1427m

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96T

CLIA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

U1427h

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MSDS:

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Packing:

96T

CLIA Kit for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

U1427d

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96T

Polyclonal Antibody for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

P1427Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

P1427Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Keratin, type II cytoskeletal 2 epidermal

Cat.:

P1427Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Keratin, type II cytoskeletal 2 epidermal

Protein for Human Keratin, type II cytoskeletal 2 epidermal

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of K22E into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-100
Plasma	99	96-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of K22E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-100%	94-102%	96-101%	94-100%
EDTA plasma(n=5)	94-102%	96-103%	97-103%	95-104%
heparin plasma(n=5)	92-106%	93-104%	92-105%	94-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Characterization of human cytokeratin 2, an epidermal cytoskeletal protein synthesized late during differentiation."
Collin C. , Moll R. , Kubicka S. , Ouhayoun J.-P. , Franke W.W.
Exp. Cell Res.202:132-141(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE;VARIANT GLY-101
tissue: Thigh epidermis.
2.

"Genomic organization and fine mapping of the keratin 2e gene (KRT2E): K2e V1 domain polymorphism and novel mutations in ichthyosis bullosa of Siemens."
Smith F.J.D. , Maingi C. , Covello S.P. , Higgins C. , Schmidt M. , Lane E.B. , Uitto J. , Leigh I.M. , McLean W.H.I.
J. Invest. Dermatol.111:817-821(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS IBS TYR-186 AND LYS-476;VARIANT GLY-101
3.

"The finished DNA sequence of human chromosome 12."
Scherer S.E. , Muzny D.M. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , Jackson A. , Khan Z.M. , Kovar-Smith C. , Lewis L.R. , more...
Nature440:346-351(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
5.

"Ontogeny and regional variability of keratin 2e (K2e) in developing human fetal skin: a unique spatial and temporal pattern of keratin expression in development."
Smith L.T. , Underwood R.A. , McLean W.H.I.
Br. J. Dermatol.140:582-591(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
6.

"Expression of keratin K2e in cutaneous and oral lesions: association with keratinocyte activation, proliferation, and keratinization."
Bloor B.K. , Tidman N. , Leigh I.M. , Odell E. , Dogan B. , Wollina U. , Ghali L. , Waseem A.
Am. J. Pathol.162:963-975(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY
7.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
8.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
9.

"Ichthyosis bullosa of Siemens -- a disease involving keratin 2e."
McLean W.H.I. , Morley S.M. , Lane E.B. , Eady R.A.J. , Griffiths W.A.D. , Paige D.G. , Harper J.I. , Higgins C. , Leigh I.M.
J. Invest. Dermatol.103:277-281(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS LYS-487
10.

"Ichthyosis bullosa of Siemens is caused by mutations in the keratin 2e gene."
Kremer H. , Zeeuwen P. , McLean W.H.I. , Mariman E.C.M. , Lane E.B. , van de Kerkhof P.C.M. , Ropers H.-H. , Steijlen P.M.
J. Invest. Dermatol.103:286-289(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS IBS PRO-181; PRO-484 AND LYS-487
11.

"Mutations in the rod domain of keratin 2e in patients with ichthyosis bullosa of Siemens."
Rothnagel J.A. , Traupe H. , Wojcik S. , Huber M. , Hohl D. , Pittelkow M.R. , Saeki H. , Ishibashi Y. , Roop D.R.
Nat. Genet.7:485-490(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS IBS ASP-487 AND LYS-487
12.

"A new keratin 2e mutation in ichthyosis bullosa of Siemens."
Jones D.O. , Watts C. , Mills C. , Sharpe G. , Marks R. , Bowden P.E.
J. Invest. Dermatol.108:354-356(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS IBS LYS-487 AND LYS-488
13.

"A novel threonine-to-proline mutation at the end of 2B rod domain in the keratin 2e chain in ichthyosis bullosa of Siemens."
Yang J.-M. , Lee S. , Bang H.-D. , Kim W.-S. , Lee E.-S. , Steinert P.M.
J. Invest. Dermatol.109:116-118(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS PRO-479
14.

"A glutamate to lysine mutation at the end of 2B rod domain of keratin 2e gene in ichthyosis bullosa of Siemens."
Yang J.-M. , Lee E.-S. , Kang H.-J. , Choi G.-S. , Yoneda K. , Jung S.-Y. , Park K.-B. , Steinert P.M. , Lee E.-S.
Acta Derm. Venereol.78:417-419(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS LYS-487
15.

"Ichthyosis bullosa of Siemens: report of a family with evidence of a keratin 2e mutation, and a review of the literature."
Basarab T. , Smith F.J. , Jolliffe V.M. , McLean W.H.I. , Neill S. , Rustin M.H. , Eady R.A.
Br. J. Dermatol.140:689-695(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS LYS-487
16.

"A novel mutation in the 1A domain of keratin 2e in ichthyosis bullosa of Siemens."
Arin M.J. , Longley M.A. , Epstein E.H. Jr. , Scott G. , Goldsmith L.A. , Rothnagel J.A. , Roop D.R.
J. Invest. Dermatol.112:380-382(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS ASN-182
17.

"Ichthyosis bullosa of Siemens resulting from a novel missense mutation near the helix termination motif of the keratin 2e gene."
Moraru R. , Cserhalmi-Friedman P.B. , Grossman M.E. , Schneiderman P. , Christiano A.M.
Clin. Exp. Dermatol.24:412-415(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS VAL-476
18.

"A novel mutation in the 2B domain of keratin 2e causing ichthyosis bullosa of Siemens."
Irvine A.D. , Smith F.J. , Shum K.W. , Williams H.C. , McLean W.H.I.
Clin. Exp. Dermatol.25:648-651(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS ASN-477
19.

"Hot spot mutations in keratin 2e suggest a correlation between genotype and phenotype in patients with ichthyosis bullosa of Siemens."
Suga Y. , Arin M.J. , Scott G. , Goldsmith L.A. , Magro C.M. , Baden L.A. , Baden H.P. , Roop D.R.
Exp. Dermatol.9:11-15(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS IBS LYS-465 AND ASP-465
20.

"A novel asparagine-->aspartic acid mutation in the rod 1A domain in keratin 2e in a Japanese family with ichthyosis bullosa of Siemens."
Takizawa Y. , Akiyama M. , Nagashima M. , Shimizu H.
J. Invest. Dermatol.114:193-195(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS ASP-186
21.

"New mutations in keratin 1 that cause bullous congenital ichthyosiform erythroderma and keratin 2e that cause ichthyosis bullosa of Siemens."
Whittock N.V. , Ashton G.H.S. , Griffiths W.A.D. , Eady R.A.J. , McGrath J.A.
Br. J. Dermatol.145:330-335(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT IBS LYS-186
22.

"Ichthyosis bullosa of Siemens: its correct diagnosis facilitated by molecular genetic testing."
Akiyama M. , Tsuji-Abe Y. , Yanagihara M. , Nakajima K. , Kodama H. , Yaosaka M. , Abe M. , Sawamura D. , Shimizu H.
Br. J. Dermatol.152:1353-1356(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS IBS PRO-484 AND LYS-487

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