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Index > Protein center > PRKCE(Gene name) > Human
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  • PRKCE (Gene name),
  • Protein kinase C epsilon type (Protein name ),  KPCE_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    PRKCE(PKCE);
    Protein name:
    Protein kinase C epsilon type;
    Alternative:
    nPKC-epsilon;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Forms a ternary complex with TRIM63 and GN2BL1 (By similarity). Interacts with DGKQ.
    Function:
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    737
    Sequence:
    50:
    MVVFNGLLKI | KICEAVSLKP | TAWSLRHAVG | PRPQTFLLDP | YIALNVDDSR | 
    100:
    IGQTATKQKT | NSPAWHDEFV | TDVCNGRKIE | LAVFHDAPIG | YDDFVANCTI | 
    150:
    QFEELLQNGS | RHFEDWIDLE | PEGRVYVIID | LSGSSGEAPK | DNEERVFRER | 
    200:
    MRPRKRQGAV | RRRVHQVNGH | KFMATYLRQP | TYCSHCRDFI | WGVIGKQGYQ | 
    250:
    CQVCTCVVHK | RCHELIITKC | AGLKKQETPD | QVGSQRFSVN | MPHKFGIHNY | 
    300:
    KVPTFCDHCG | SLLWGLLRQG | LQCKVCKMNV | HRRCETNVAP | NCGVDARGIA | 
    350:
    KVLADLGVTP | DKITNSGQRR | KKLIAGAESP | QPASGSSPSE | EDRSKSAPTS | 
    400:
    PCDQEIKELE | NNIRKALSFD | NRGEEHRAAS | SPDGQLMSPG | ENGEVRQGQA | 
    450:
    KRLGLDEFNF | IKVLGKGSFG | KVMLAELKGK | DEVYAVKVLK | KDVILQDDDV | 
    500:
    DCTMTEKRIL | ALARKHPYLT | QLYCCFQTKD | RLFFVMEYVN | GGDLMFQIQR | 
    550:
    SRKFDEPRSR | FYAAEVTSAL | MFLHQHGVIY | RDLKLDNILL | DAEGHCKLAD | 
    600:
    FGMCKEGILN | GVTTTTFCGT | PDYIAPEILQ | ELEYGPSVDW | WALGVLMYEM | 
    650:
    MAGQPPFEAD | NEDDLFESIL | HDDVLYPVWL | SKEAVSILKA | FMTKNPHKRL | 
    700:
    GCVASQNGED | AIKQHPFFKE | IDWVLLEQKK | IKPPFKPRIK | TKRDVNNFDQ | 
    737:
    DFTREEPVLT | LVDEAIVKQI | NQEEFKGFSY | FGEDLMP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    MIM:
    String:
    UniGene:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human Protein kinase C epsilon type
    Cat.:
    E8827h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Protein kinase C epsilon type
    Cat.:
    E8827m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Protein kinase C epsilon type
    Cat.:
    E8827r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Protein kinase C epsilon type
    MSDS:
    Please sign in first.
    CLIA Kit for Human Protein kinase C epsilon type
    Cat.:
    U8827r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Protein kinase C epsilon type
    Cat.:
    U8827h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Protein kinase C epsilon type
    MSDS:
    Please sign in first.
    CLIA Kit for Human Protein kinase C epsilon type
    Cat.:
    U8827m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Protein kinase C epsilon type
    Polyclonal Antibody for Human Protein kinase C epsilon type
    Cat.:
    P8827Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein kinase C epsilon type
    Cat.:
    P8827Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein kinase C epsilon type
    Monoclonal Antibody for Human Protein kinase C epsilon type
    Monoclonal Antibody for Human Protein kinase C epsilon type
    Monoclonal Antibody for Human Protein kinase C epsilon type
    Monoclonal Antibody for Human Protein kinase C epsilon type
    Protein for Human Protein kinase C epsilon type
    Protein for Human Protein kinase C epsilon type
    Protein for Human Protein kinase C epsilon type
    Protein for Human Protein kinase C epsilon type

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    s
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.3%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of KPCE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    96

    93-103

    Plasma

    98

    93-104

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KPCE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    89-105%

    90-101%

    94-100%

    92-102%

    EDTA plasma(n=5)

    91-107%

    90-104%

    92-103%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-101%

    95-102%

    91-105%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      SeattleSNPs variation discovery resource
      Submitted (2007-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
      Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    4. 4.
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    5. 5.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    6. 6.
      "PKC epsilon-related kinase associates with and phosphorylates cytokeratin 8 and 18."
      Omary M.B. , Baxter G.T. , Chou C.F. , Riopel C.L. , Lin W.Y. , Strulovici B.
      J. Cell Biol.117:583-593(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF KRT8
    7. 7.
      "Regulation of novel protein kinase C epsilon by phosphorylation."
      Cenni V. , Doeppler H. , Sonnenburg E.D. , Maraldi N. , Newton A.C. , Toker A.
      Biochem. J.363:537-545(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-566 AND SER-729;MUTAGENESIS OF LYS-437; THR-566; THR-710 AND SER-729
    8. 8.
      "Direct phosphorylation of capsaicin receptor VR1 by protein kinase Cepsilon and identification of two target serine residues."
      Numazaki M. , Tominaga T. , Toyooka H. , Tominaga M.
      J. Biol. Chem.277:13375-13378(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TRPV1
    9. 9.
      "Phosphorylation of IQGAP1 modulates its binding to Cdc42, revealing a new type of rho-GTPase regulator."
      Grohmanova K. , Schlaepfer D. , Hess D. , Gutierrez P. , Beck M. , Kroschewski R.
      J. Biol. Chem.279:48495-48504(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF IQGAP1
    10. 10.
      "Translocation of diacylglycerol kinase theta from cytosol to plasma membrane in response to activation of G protein-coupled receptors and protein kinase C."
      van Baal J. , de Widt J. , Divecha N. , van Blitterswijk W.J.
      J. Biol. Chem.280:9870-9878(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DGKQ
    11. 11.
      "Trif-related adapter molecule is phosphorylated by PKCepsilon during Toll-like receptor 4 signaling."
      McGettrick A.F. , Brint E.K. , Palsson-McDermott E.M. , Rowe D.C. , Golenbock D.T. , Gay N.J. , Fitzgerald K.A. , O'Neill L.A.J.
      Proc. Natl. Acad. Sci. U.S.A.103:9196-9201(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    12. 12.
      "Protein kinase Cepsilon interacts with signal transducers and activators of transcription 3 (Stat3), phosphorylates Stat3Ser727, and regulates its constitutive activation in prostate cancer."
      Aziz M.H. , Manoharan H.T. , Church D.R. , Dreckschmidt N.E. , Zhong W. , Oberley T.D. , Wilding G. , Verma A.K.
      Cancer Res.67:8828-8838(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF STAT3;INTERACTION WITH STAT3
    13. 13.
      "Protein kinase C alpha and epsilon differentially modulate hepatocyte growth factor-induced epithelial proliferation and migration."
      Sharma G.D. , Kakazu A. , Bazan H.E.
      Exp. Eye Res.85:289-297(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CELL MOTILITY;SUBCELLULAR LOCATION
    14. 14.
      "Protein kinase C epsilon regulates gamma-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of gamma2 subunits."
      Qi Z.H. , Song M. , Wallace M.J. , Wang D. , Newton P.M. , McMahon T. , Chou W.H. , Zhang C. , Shokat K.M. , Messing R.O.
      J. Biol. Chem.282:33052-33063(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF GABRG2
    15. 15.
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      Akita Y.
      J. Biochem.132:847-852(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
    16. 16.
      "TNF-related apoptosis-inducing ligand (TRAIL) and erythropoiesis: a role for PKC epsilon."
      Vitale M. , Gobbi G. , Mirandola P. , Ponti C. , Sponzilli I. , Rinaldi L. , Manzoli F.A.
      Eur. J. Histochem.50:15-18(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION IN APOPTOSIS
    17. 17.
      "Protein kinase Cepsilon: function in neurons."
      Shirai Y. , Adachi N. , Saito N.
      FEBS J.275:3988-3994(2008) [PubMed] [Europe PMC] [Abstract]
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    18. 18.
      "Protein kinase Cepsilon: multiple roles in the function of, and signaling mediated by, the cytoskeleton."
      Akita Y.
      FEBS J.275:3995-4004(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION IN CYTOSKELETON
    19. 19.
      "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
      Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-228; THR-309; SER-329; SER-388 AND THR-710;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    20. 20.
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      tissue: Cervix carcinoma.
    21. 21.
      "The substrates and binding partners of protein kinase Cepsilon."
      Newton P.M. , Messing R.O.
      Biochem. J.427:189-196(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    22. 22.
      "The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases."
      Chen Y. , Tian Q.
      Front. Med.5:70-76(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION IN NEURONS
    23. 23.
      "PKC-delta and PKC-epsilon: Foes of the same family or strangers?"
      Duquesnes N. , Lezoualc'h F. , Crozatier B.
      J. Mol. Cell. Cardiol.51:665-673(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    24. 24.
      "Recognition of an intra-chain tandem 14-3-3 binding site within PKCepsilon."
      Kostelecky B. , Saurin A.T. , Purkiss A. , Parker P.J. , McDonald N.Q.
      EMBO Rep.10:983-989(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 342-372;PHOSPHORYLATION AT SER-346 AND SER-368
    25. 25.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-143
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