Pyruvate kinase isozymes M1/M2 (Protein name
), KPYM_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
PKM2(OIP3;PK2;PK3;PKM);
Protein name:
Pyruvate kinase isozymes M1/M2;
Alternative:
Opa-interacting protein 3(OIP-3);Cytosolic thyroid hormone-binding protein(CTHBP);Pyruvate kinase muscle isozyme;Pyruvate kinase 2/3;Tumor M2-PK;Thyroid hormone-binding protein 1(THBP1);p58;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML.
Function:
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Subcellular Location:
Cytoplasm
Nucleus
Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
Monoclonal Antibody for Human Pyruvate kinase isozymes M1/M2
Monoclonal Antibody for Human Pyruvate kinase isozymes M1/M2
Monoclonal Antibody for Human Pyruvate kinase isozymes M1/M2
Monoclonal Antibody for Human Pyruvate kinase isozymes M1/M2
Monoclonal Antibody for Human Pyruvate kinase isozymes M1/M2
Protein for Human Pyruvate kinase isozymes M1/M2
Protein for Human Pyruvate kinase isozymes M1/M2
Protein for Human Pyruvate kinase isozymes M1/M2
Protein for Human Pyruvate kinase isozymes M1/M2
Protein for Human Pyruvate kinase isozymes M1/M2
R&D Technical Data
s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M2);PROTEIN SEQUENCE OF 70-98;SUBUNIT;ENZYME REGULATION
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS M1 AND 3)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM M2)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM M2);VARIANT VAL-204
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Cited for: PROTEIN SEQUENCE OF 2-43; 57-73; 93-115; 126-135; 167-186; 231-246; 271-311; 401-422; 448-455 AND 490-498;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY
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Cited for: PROTEIN SEQUENCE OF 2-18;CATALYTIC ACTIVITY;ENZYME REGULATION;BIOPHYSICOCHEMICAL PROPERTIES;SUBUNIT;INTERACTION WITH THYROID HORMONE
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Cited for: PROTEIN SEQUENCE OF 74-89;IDENTIFICATION BY MASS SPECTROMETRY
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 368-531 (ISOFORM M2)
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Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: INTERACTION WITH PML;ENZYME REGULATION;SUBUNIT;SUBCELLULAR LOCATION
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Cited for: INTERACTION WITH POU5F1;IDENTIFICATION BY MASS SPECTROMETRY;FUNCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
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Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37; TYR-175 AND THR-195;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-62; LYS-89; LYS-166; LYS-266 AND LYS-433;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: INTERACTION WITH EGLN3 AND HIF1A;SUBCELLULAR LOCATION;INDUCTION;FUNCTION;IDENTIFICATION BY MASS SPECTROMETRY;HYDROXYLATION AT PRO-403 AND PRO-408;MUTAGENESIS OF PRO-403 AND PRO-408
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.82 ANGSTROMS) OF ISOFORM M2 IN COMPLEX WITH OXALATE AND FBP;CATALYTIC ACTIVITY;SUBUNIT;ENZYME MECHANISM;ENZYME REGULATION;BIOPHYSICOCHEMICAL PROPERTIES
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Cited for: X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 14-531 ALONE AND IN COMPLEX WITH FBP;ENZYME REGULATION
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Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 2-531;ENZYME REGULATION BY SERINE;MAGNESIUM-BINDING SITES;SUBUNIT;MUTAGENESIS OF SER-437 AND HIS-464