LEPR ELISA Kit for Human(Homo sapiens) | EIAab.comLEPR ELISA Kit for Human(Homo sapiens) | EIAab.com

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LEPR (Gene name),

Leptin receptor (Protein name ), LEPR_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

LEPR(DB;OBR);

Protein name:

Leptin receptor(LEP-R);

Alternative:

OB receptor(OB-R);HuB219;

CD:

CD295;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling.

Function:

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.

Subcellular Location:

Isoform E Secreted

Protein Attributes:

Sequence length:

1165

Sequence:

50:

MICQKFCVVL | LHWEFIYVIT | AFNLSYPITP | WRFKLSCMPP | NSTYDYFLLP |

100:

AGLSKNTSNS | NGHYETAVEP | KFNSSGTHFS | NLSKTTFHCC | FRSEQDRNCS |

150:

LCADNIEGKT | FVSTVNSLVF | QQIDANWNIQ | CWLKGDLKLF | ICYVESLFKN |

200:

LFRNYNYKVH | LLYVLPEVLE | DSPLVPQKGS | FQMVHCNCSV | HECCECLVPV |

250:

PTAKLNDTLL | MCLKITSGGV | IFQSPLMSVQ | PINMVKPDPP | LGLHMEITDD |

300:

GNLKISWSSP | PLVPFPLQYQ | VKYSENSTTV | IREADKIVSA | TSLLVDSILP |

350:

GSSYEVQVRG | KRLDGPGIWS | DWSTPRVFTT | QDVIYFPPKI | LTSVGSNVSF |

400:

HCIYKKENKI | VPSKEIVWWM | NLAEKIPQSQ | YDVVSDHVSK | VTFFNLNETK |

450:

PRGKFTYDAV | YCCNEHECHH | RYAELYVIDV | NINISCETDG | YLTKMTCRWS |

500:

TSTIQSLAES | TLQLRYHRSS | LYCSDIPSIH | PISEPKDCYL | QSDGFYECIF |

550:

QPIFLLSGYT | MWIRINHSLG | SLDSPPTCVL | PDSVVKPLPP | SSVKAEITIN |

600:

IGLLKISWEK | PVFPENNLQF | QIRYGLSGKE | VQWKMYEVYD | AKSKSVSLPV |

650:

PDLCAVYAVQ | VRCKRLDGLG | YWSNWSNPAY | TVVMDIKVPM | RGPEFWRIIN |

700:

GDTMKKEKNV | TLLWKPLMKN | DSLCSVQRYV | INHHTSCNGT | WSEDVGNHTK |

750:

FTFLWTEQAH | TVTVLAINSI | GASVANFNLT | FSWPMSKVNI | VQSLSAYPLN |

800:

SSCVIVSWIL | SPSDYKLMYF | IIEWKNLNED | GEIKWLRISS | SVKKYYIHDH |

850:

FIPIEKYQFS | LYPIFMEGVG | KPKIINSFTQ | DDIEKHQSDA | GLYVIVPVII |

900:

SSSILLLGTL | LISHQRMKKL | FWEDVPNPKN | CSWAQGLNFQ | KPETFEHLFI |

950:

KHTASVTCGP | LLLEPETISE | DISVDTSWKN | KDEMMPTTVV | SLLSTTDLEK |

1000:

GSVCISDQFN | SVNFSEAEGT | EVTYEDESQR | QPFVKYATLI | SNSKPSETGE |

1050:

EQGLINSSVT | KCFSSKNSPL | KDSFSNSSWE | IEAQAFFILS | DQHPNIISPH |

1100:

LTFSEGLDEL | LKLEGNFPEE | NNDKKSIYYL | GVTSIKKRES | GVLLTDKSRV |

1150:

SCPFPAPCLF | TDIRVLQDSC | SHFVENNINL | GTSSKKTFAS | YMPQFQTCST |

1165:

QTHKIMENKM | CDLTV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Pfam:

UniGene:

STRING:

9606.ENSP00000330393

MIM:

String:

KEGG:

Uniprot:

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ELISA Kit for Human LEP-R

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E0083m

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96T

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ELISA Kit for Human LEP-R

Cat.:

E0083p

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96T

ELISA Kit for Human LEP-R

Cat.:

E0083h

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ELISA Kit for Human LEP-R

Cat.:

E0083r

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96T

CLIA Kit for Human LEP-R

Cat.:

U0083r

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96T

CLIA Kit for Human LEP-R

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U0083p

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96T

CLIA Kit for Human LEP-R

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U0083h

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CLIA Kit for Human LEP-R

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U0083m

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96T

Polyclonal Antibody for Human LEP-R

Cat.:

P0083Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human LEP-R

Cat.:

P0083Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human LEP-R

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P0083Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human LEP-R

Monoclonal Antibody for Human LEP-R

Monoclonal Antibody for Human LEP-R

Monoclonal Antibody for Human LEP-R

Monoclonal Antibody for Human LEP-R

Protein for Human LEP-R

Protein for Human LEP-R

Protein for Human LEP-R

Protein for Human LEP-R

R&D Technical Data

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s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.9%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of LEPR into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-101
Plasma	98	95-102

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEPR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-99%	95-100%	93-102%	92-103%
Saliva(n=3)	91-103%	93-102%	94-100%	93-101%
heparin plasma(n=5)	95-100%	91-104%	95-100%	94-100%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Identification and expression cloning of a leptin receptor, OB-R."
Tartaglia L.A. , Dembski M. , Weng X. , Deng N. , Culpepper J. , Devos R. , Richards G.J. , Campfield L.A. , Clark F.T. , Deeds J. , Muir C. , Sanker S. , Moriarty A. , Moore K.J. , Smutko J.S. , Mays G.G. , Woolf E.A. , Monroe C.A. , more...
Cell83:1263-1271(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND E)
tissue: Brain.
2.

"A role for leptin and its cognate receptor in hematopoiesis."
Bennett B.D. , Solar G.P. , Yuan J.Q. , Mathias J. , Thomas G.R. , Matthews W.
Curr. Biol.6:1170-1180(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C)
tissue: Fetal liver.
3.

"Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction."
Cioffi J.A. , Shafer A.W. , Zupancic T.J. , Smith-Gbur J. , Mikhail A. , Platika D. , Snodgrass H.R.
Nat. Med.2:585-589(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; C AND D);VARIANTS ARG-109 AND ARG-223
tissue: Fetal liver.
4.

"Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians."
Thompson D.B. , Ravussin E. , Bennett P.H. , Bogardus C.
Hum. Mol. Genet.6:675-679(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B);VARIANTS ARG-109 AND ARG-223
5.

"Cloning and characterization of a human leptin receptor using a biologically active leptin immunoadhesin."
Luoh S.-M. , Di Marco F. , Levin N. , Armanini M. , Xie M.H. , Nelson C. , Bennett G.L. , Williams M. , Spencer S.A. , Gurney A. , de Sauvage F.J.
J. Mol. Endocrinol.18:77-85(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A)
6.

"A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction."
Clement K. , Vaisse C. , Lahlou N. , Cabrol S. , Pelloux V. , Cassuto D. , Gourmelen M. , Dina C. , Chambaz J. , Lacorte J.M. , Basdevant A. , Bougneres P. , Lebouc Y. , Froguel P. , Guy-Grand B.
Nature392:398-401(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN LEPRD
7.

"The long terminal repeat of an endogenous retrovirus induces alternative splicing and encodes an additional carboxy-terminal sequence in the human leptin receptor."
Kapitonov V.V. , Jurka J.
J. Mol. Evol.48:248-251(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING DUE TO AN ENDOGENOUS RETROVIRUS
8.

"Enhancing leptin response by preventing SH2-containing phosphatase 2 interaction with Ob receptor."
Carpenter L.R. , Farruggella T.J. , Symes A. , Karow M.L. , Yancopoulos G.D. , Stahl N.
Proc. Natl. Acad. Sci. U.S.A.95:6061-6066(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PTPN11;MUTAGENESIS OF TYR-986; 1078-TYR-TYR-1079 AND TYR-1141
9.

"Human leptin receptor. Determination of disulfide structure and N-glycosylation sites of the extracellular domain."
Haniu M. , Arakawa T. , Bures E.J. , Young Y. , Hui J.O. , Rohde M.F. , Welcher A.A. , Horan T.
J. Biol. Chem.273:28691-28699(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-23; ASN-41; ASN-56; ASN-73; ASN-81; ASN-98; ASN-187; ASN-206; ASN-276; ASN-347; ASN-397; ASN-516; ASN-624; ASN-659; ASN-688; ASN-697; ASN-728 AND ASN-750;DISULFIDE BONDS;PARTIAL PROTEIN SEQUENCE
10.

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-276 AND ASN-516
tissue: Plasma.
11.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-276 AND ASN-397
tissue: Liver.
12.

"Structure of the human obesity receptor leptin-binding domain reveals the mechanism of leptin antagonism by a monoclonal antibody."
Carpenter B. , Hemsworth G.R. , Wu Z. , Maamra M. , Strasburger C.J. , Ross R.J. , Artymiuk P.J.
Structure20:487-497(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 428-633 IN COMPLEX WITH ANTIBODY;LEPTIN-BINDING REGION;DISULFIDE BONDS
13.

"The hypothalamic leptin receptor in humans: identification of incidental sequence polymorphisms and absence of the db/db mouse and fa/fa rat mutations."
Considine R.V. , Considine E.L. , Williams C.J. , Hyde T.M. , Caro J.F.
Diabetes45:992-994(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-223
14.

"Amino acid variants in the human leptin receptor: lack of association to juvenile onset obesity."
Echwald S.M. , Soerensen T.D. , Soerensen T.I. , Tybjaerg-Hansen A. , Andersen T. , Chung W.K. , Leibel R.L. , Pedersen O.
Biochem. Biophys. Res. Commun.233:248-252(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-109; ARG-204; ARG-223 AND ASN-656
15.

"Exonic and intronic sequence variation in the human leptin receptor gene (LEPR)."
Chung W.K. , Power-Kehoe L. , Chua M. , Chu F. , Aronne L. , Huma Z. , Sothern M. , Udall J.N. , Kahle B. , Leibel R.L.
Diabetes46:1509-1511(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-109; ARG-223 AND ASN-656
16.

"Leptin receptor gene variation and obesity: lack of association in a white British male population."
Gotoda T. , Manning B.S. , Goldstone A.P. , Imrie H. , Evans A.L. , Strosberg A.D. , McKeigue P.M. , Scott J. , Aitman T.J.
Hum. Mol. Genet.6:869-876(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-109; ARG-223 AND ASN-656
17.

"Transmission disequilibrium and sequence variants at the leptin receptor gene in extremely obese German children and adolescents."
Roth H. , Korn T. , Rosenkranz K. , Hinney A. , Ziegler A. , Kunz J. , Siegfried W. , Mayer H. , Hebebrand J. , Grzeschik K.-H.
Hum. Genet.103:540-546(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-109; ARG-223; ASN-656 AND THR-675
18.

"DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome."
Ley T.J. , Mardis E.R. , Ding L. , Fulton B. , McLellan M.D. , Chen K. , Dooling D. , Dunford-Shore B.H. , McGrath S. , Hickenbotham M. , Cook L. , Abbott R. , Larson D.E. , Koboldt D.C. , Pohl C. , Smith S. , Hawkins A. , Abbott S. , more...
Nature456:66-72(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-109 AND ARG-223

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