Lepr ELISA Kit for Rat(Rattus norvegicus) | EIAab.comLepr ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Lepr (Gene name),

Leptin receptor (Protein name ), LEPR_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Lepr(Fa;Obr);

Protein name:

Leptin receptor(LEP-R);

Alternative:

OB receptor(OB-R);

CD:

CD295;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

On leptin stimulation, homodimerizes (By similarity). The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling.

Function:

The putative soluble isoform E could function as a transport protein.

Subcellular Location:

Isoform E Secreted

Protein Attributes:

Sequence length:

1162

Sequence:

50:

MTCQKFYVVL | LHWEFLYVIT | ALNLAYPTSP | WRFKLFCAPP | STTDDSFLSP |

100:

AGVPNNTSSL | KGASEALVEA | KFNSTGIYVS | ELSKTIFHCC | FGNEQGQNCS |

150:

ALTGNTEGKT | LASVVKPLVF | RQLGVNWDIE | CWMKGDLTLF | ICHMEPLLKN |

200:

PFKNYDSKVH | LLYDLPEVID | DLPLPPLKDS | FQTVQCNCSV | RECECHVPVP |

250:

RAKVNYALLM | YLEITSAGVS | FQSPLMSLQP | MLVVKPDPPL | GLRMEVTDDG |

300:

NLKISWDSQT | KAPFPLQYQV | KYLENSTIVR | EAAEIVSDTS | LLVDSVLPGS |

350:

SYEVQVRSKR | LDGSGVWSDW | SLPQLFTTQD | VMYFPPKILT | SVGSNASFCC |

400:

IYKNENQTIS | SKQIVWWMNL | AEKIPETQYN | TVSDHISKVT | FSNLKATRPR |

450:

GKFTYDAVYC | CNEQACHHRY | AELYVIDVNI | NISCETDGYL | TKMTCRWSPS |

500:

TIQSLVGSTV | QLRYHRRSLY | CPDNPSIRPT | SELKNCVLQT | DGFYECVFQP |

550:

IFLLSGYTMW | IRINHSLGSL | DSPPTCVLPD | SVVKPLPPSN | VKAEITINTG |

600:

LLKVSWEKPV | FPENNLQFQI | RYGLNGKEIQ | WKTHEVFDAK | SKSASLPVSD |

650:

LCAVYVVQVR | CRRLDGLGYW | SNWSSPAYTL | VMDVKVPMRG | PEFWRIMDGD |

700:

ITKKERNVTL | LWKPLMKNDS | LCSVRRYVVK | HRTAHNGTWS | QDVGNQTNLT |

750:

FLWAESAHTV | TVLAINSIGA | SLVNFNLTFS | WPMSKVNAVQ | SLSAYPLSSS |

800:

CVILSWTLSP | NDYSLLYLVI | EWKNLNDDDG | MKWLRIPSNV | NKYYIHDNFI |

850:

PIEKYQFSLY | PVFMEGVGKP | KIINGFTKDD | IAKQQNDAGL | YVIVPIIISS |

900:

CVLLLGTLLI | SHQRMKKLFW | DDVPNPKNCS | WAQGLNFQKP | ETFEHLFTKH |

950:

AESVIFGPLL | LEPEPVSEEI | SVDTAWKNKD | EMVPAAMVSL | LLTTPDSTRG |

1000:

SICISDQCNS | ANFSGAQSTQ | GTCEDECQSQ | PSVKYATLVS | NVKTVETDEE |

1050:

QGAIHSSVSQ | CIARKHSPLR | QSFSSNSWEI | EAQAFFLLSD | HPPNVISPQL |

1100:

SFSGLDELLE | LEGNFPEENH | GEKSVYYLGV | SSGNKRENDM | LLTDEAGVLC |

1150:

PFPAHCLFSD | IRILQESCSH | FVENNLNLGT | SGKNFVPYMP | QFQSCSTHSH |

1162:

KIIENKMCDL | TV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

Pfam:

UniGene:

Uniprot:

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ELISA Kit for Rat LEP-R

Cat.:

E0083m

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Rat LEP-R

Cat.:

E0083p

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MSDS:

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Packing:

96T

ELISA Kit for Rat LEP-R

Cat.:

E0083h

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Packing:

96T

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ELISA Kit for Rat LEP-R

Cat.:

E0083r

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MSDS:

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Packing:

96T

CLIA Kit for Rat LEP-R

Cat.:

U0083r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Rat LEP-R

Cat.:

U0083p

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MSDS:

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Packing:

96T

CLIA Kit for Rat LEP-R

Cat.:

U0083h

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MSDS:

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Packing:

96T

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CLIA Kit for Rat LEP-R

Cat.:

U0083m

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Packing:

96T

Polyclonal Antibody for Rat LEP-R

Cat.:

P0083Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat LEP-R

Cat.:

P0083Rb-r

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat LEP-R

Cat.:

P0083Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat LEP-R

Monoclonal Antibody for Rat LEP-R

Monoclonal Antibody for Rat LEP-R

Monoclonal Antibody for Rat LEP-R

Monoclonal Antibody for Rat LEP-R

Protein for Rat LEP-R

Protein for Rat LEP-R

Protein for Rat LEP-R

Protein for Rat LEP-R

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.9%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

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Recovery was determined by spiking various levels of LEPR into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-101
Plasma	98	95-102

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEPR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-99%	95-100%	93-102%	92-103%
Saliva(n=3)	91-103%	93-102%	94-100%	93-101%
heparin plasma(n=5)	95-100%	91-104%	95-100%	94-100%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Leptin receptor missense mutation in the fatty Zucker rat."
Phillips M.S. , Liu Q. , Hammond H.A. , Dugan V. , Hey P.J. , Caskey C.T. , Hess J.F.
Nat. Genet.13:18-19(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B);VARIANT FA PRO-269
strain: Zucker.
tissue: Hypothalamus.
2.

"Substitution at codon 269 (glutamine --> proline) of the leptin receptor (OB-R) cDNA is the only mutation found in the Zucker fatty (fa/fa) rat."
Iida M. , Murakami T. , Ishida K. , Mizuno A. , Kuwajima M. , Shima K.
Biochem. Biophys. Res. Commun.224:597-604(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B);VARIANT FA PRO-269
strain: Sprague-Dawley.
strain: Zucker fatty.
tissue: Brain.
3.

"Molecular cloning of rat leptin receptor isoform complementary DNAs -- identification of a missense mutation in Zucker fatty (fa/fa) rats."
Takaya K. , Ogawa Y. , Isse N. , Okazaki T. , Satoh N. , Masuzaki H. , Mori K. , Tamura N. , Hosoda K. , Nakao K.
Biochem. Biophys. Res. Commun.225:75-83(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND E);VARIANT FA PRO-269
strain: Sprague-Dawley.
strain: Zucker fatty.
4.

"Cloning of the rat leptin receptor."
Karlsson C. , Lindell K. , Robinson I.C.A.F. , Carlsson L.M.S. , Carlsson B.
Submitted (1996-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B)
5.

"Phenotype-linked amino acid alteration in leptin receptor cDNA from Zucker fatty (fa/fa) rat."
Iida M. , Murakami T. , Ishida K. , Mizuno A. , Kuwajima M. , Shima K.
Biochem. Biophys. Res. Commun.222:19-26(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A);VARIANT FA PRO-269
strain: Sprague-Dawley.
strain: Zucker fatty.
6.

"Molecular cloning, sequencing, and recombinant expression of the long form of the rat leptin receptor isolated from whole spleen RNA."
Park J.H. , Ju S.K. , Na S.Y. , You K.H. , Kim K.L.
Submitted (2000-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B)
strain: Sprague-Dawley.
tissue: Spleen.
7.

"A novel leptin receptor isoform in rat."
Wang M.-Y. , Zhou Y.T. , Newgard C.B. , Unger R.H.
FEBS Lett.392:87-90(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM F)
strain: Sprague-Dawley.
tissue: Brain.
8.

"Analysis of rat leptin receptor gene."
Morishita T. , Hidaka T. , Kuzuyama T. , Noguchi T.
Submitted (1998-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-123
9.

"Increase in serum leptin and uterine leptin receptor messenger RNA levels during pregnancy in rats."
Chien E.K. , Hara M. , Rouard M. , Yano H. , Phillippe M. , Polonsky K.S. , Bell G.I.
Biochem. Biophys. Res. Commun.237:476-480(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 557-802 AND 843-892 (ISOFORMS C AND E)
strain: Sprague-Dawley.
10.

"Identification of a leptin receptor in islet."
Ma Z.
Submitted (1996-08) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 694-878
strain: Sprague-Dawley.
tissue: Pancreas.
11.

"Leptin receptor gene expression in rat kidney."
Totsune K. , Takahashi K. , Mackenzie H.S. , Murakami O. , Arihara Z. , Sone M. , Satoh F. , Mouri T. , Brenner B.M. , Ito S.
Submitted (2000-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 821-894 (ISOFORM A)
strain: Wistar Munich.
tissue: Kidney.
12.

"Phenotype of fatty due to Gln269Pro mutation in the leptin receptor (Lepr)."
Chua S.C. Jr. , White D.W. , Wu-Peng X.S. , Liu S.M. , Okada N. , Kershaw E.E. , Chung W.K. , Power-Kehoe L. , Chua M. , Tartaglia L.A. , Leibel R.L.
Diabetes45:1141-1143(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT FA PRO-269

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