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Index > Protein center > MCM2(Gene name) > Human
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  • MCM2 (Gene name),
  • DNA replication licensing factor MCM2 (Protein name ),  MCM2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    MCM2(BM28;CCNL1;CDCL1;KIAA0030);
    Protein name:
    DNA replication licensing factor MCM2;
    Alternative:
    Nuclear protein BM28;Minichromosome maintenance protein 2 homolog;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10.
    Function:
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    Subcellular Location:
    Nucleus
    Protein Attributes:
    Sequence length:
    904
    Sequence:
    50:
    MAESSESFTM | ASSPAQRRRG | NDPLTSSPGR | SSRRTDALTS | SPGRDLPPFE | 
    100:
    DESEGLLGTE | GPLEEEEDGE | ELIGDGMERD | YRAIPELDAY | EAEGLALDDE | 
    150:
    DVEELTASQR | EAAERAMRQR | DREAGRGLGR | MRRGLLYDSD | EEDEERPARK | 
    200:
    RRQVERATED | GEEDEEMIES | IENLEDLKGH | SVREWVSMAG | PRLEIHHRFK | 
    250:
    NFLRTHVDSH | GHNVFKERIS | DMCKENRESL | VVNYEDLAAR | EHVLAYFLPE | 
    300:
    APAELLQIFD | EAALEVVLAM | YPKYDRITNH | IHVRISHLPL | VEELRSLRQL | 
    350:
    HLNQLIRTSG | VVTSCTGVLP | QLSMVKYNCN | KCNFVLGPFC | QSQNQEVKPG | 
    400:
    SCPECQSAGP | FEVNMEETIY | QNYQRIRIQE | SPGKVAAGRL | PRSKDAILLA | 
    450:
    DLVDSCKPGD | EIELTGIYHN | NYDGSLNTAN | GFPVFATVIL | ANHVAKKDNK | 
    500:
    VAVGELTDED | VKMITSLSKD | QQIGEKIFAS | IAPSIYGHED | IKRGLALALF | 
    550:
    GGEPKNPGGK | HKVRGDINVL | LCGDPGTAKS | QFLKYIEKVS | SRAIFTTGQG | 
    600:
    ASAVGLTAYV | QRHPVSREWT | LEAGALVLAD | RGVCLIDEFD | KMNDQDRTSI | 
    650:
    HEAMEQQSIS | ISKAGIVTSL | QARCTVIAAA | NPIGGRYDPS | LTFSENVDLT | 
    700:
    EPIISRFDIL | CVVRDTVDPV | QDEMLARFVV | GSHVRHHPSN | KEEEGLANGS | 
    750:
    AAEPAMPNTY | GVEPLPQEVL | KKYIIYAKER | VHPKLNQMDQ | DKVAKMYSDL | 
    800:
    RKESMATGSI | PITVRHIESM | IRMAEAHARI | HLRDYVIEDD | VNMAIRVMLE | 
    850:
    SFIDTQKFSV | MRSMRKTFAR | YLSFRRDNNE | LLLFILKQLV | AEQVTYQRNR | 
    900:
    FGAQQDTIEV | PEKDLVDKAR | QINIHNLSAF | YDSELFRMNK | FSHDLKRKMI | 
    904:
    LQQF
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    KEGG:
    UniGene:
    Pfam:
    MIM:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human DNA replication licensing factor MCM2
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    E1768h
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    ELISA Kit for Human DNA replication licensing factor MCM2
    Cat.:
    E1768m
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human DNA replication licensing factor MCM2
    Cat.:
    U1768h
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Human DNA replication licensing factor MCM2
    Cat.:
    U1768m
    Price:
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human DNA replication licensing factor MCM2
    Cat.:
    P1768Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human DNA replication licensing factor MCM2
    Cat.:
    P1768Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human DNA replication licensing factor MCM2
    Monoclonal Antibody for Human DNA replication licensing factor MCM2
    Protein for Human DNA replication licensing factor MCM2
    Protein for Human DNA replication licensing factor MCM2

    R&D Technical Data
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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division."
      Todorov I.T. , Pepperkok R. , Philipova R.N. , Kearsey S.E. , Ansorge W. , Werner D.
      J. Cell Sci.107:253-265(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;SUBCELLULAR LOCATION;VARIANT GLN-166
      tissue: Colon carcinoma.
    2. 2.
      "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1."
      Nomura N. , Miyajima N. , Sazuka T. , Tanaka A. , Kawarabayasi Y. , Sato S. , Nagase T. , Seki N. , Ishikawa K. , Tabata S.
      DNA Res.1:27-35(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Bone marrow.
    3. 3.
      NIEHS SNPs program
      Submitted (2004-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS GLU-68; PHE-135; THR-396; MET-667 AND THR-727
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS ARG-501 AND THR-727
      tissue: Brain.
      tissue: Lung.
      tissue: Lymph.
      tissue: Muscle.
      tissue: Placenta.
      tissue: Uterus.
    5. 5.
      "Homo sapiens DNA replication licensing factor (huMCM2)."
      Mimura S. , Nishimoto S. , Kubota Y. , Takisawa H. , Nojima H.
      Submitted (1996-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-904
      tissue: Cervix carcinoma.
    6. 6.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-904
    7. 7.
      "The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21."
      Mincheva A. , Todorov I.T. , Werner D. , Fink T.M. , Lichter P.
      Cytogenet. Cell Genet.65:276-277(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHROMOSOMAL LOCATION
    8. 8.
      "A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex."
      Ishimi Y.
      J. Biol. Chem.272:24508-24513(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE MCM2-7 COMPLEX
    9. 9.
      "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase."
      Izumi M. , Yanagi K. , Mizuno T. , Yokoi M. , Kawasaki Y. , Moon K.Y. , Hurwitz J. , Yatagai F. , Hanaoka F.
      Nucleic Acids Res.28:4769-4777(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MCM10
    10. 10.
      "Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases."
      Cortez D. , Glick G. , Elledge S.J.
      Proc. Natl. Acad. Sci. U.S.A.101:10078-10083(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-108;MUTAGENESIS OF SER-108
    11. 11.
      "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
      Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    12. 12.
      "Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells."
      Tsuji T. , Ficarro S.B. , Jiang W.
      Mol. Biol. Cell17:4459-4472(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-27; SER-41; SER-53; SER-108 AND SER-139;MUTAGENESIS OF SER-27; SER-41 AND SER-139;IDENTIFICATION IN THE MCM2-7 COMPLEX;ATPASE ACTIVITY OF THE MCM2-7 COMPLEX
    13. 13.
      "ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation."
      Doyon Y. , Cayrou C. , Ullah M. , Landry A.-J. , Cote V. , Selleck W. , Lane W.S. , Tan S. , Yang X.-J. , Cote J.
      Mol. Cell21:51-64(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KAT7
    14. 14.
      "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
      Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27 AND SER-41;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    15. 15.
      "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
      Giorgianni F. , Zhao Y. , Desiderio D.M. , Beranova-Giorgianni S.
      Electrophoresis28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Prostate cancer.
    16. 16.
      "Cdc7 is an active kinase in human cancer cells undergoing replication stress."
      Tenca P. , Brotherton D. , Montagnoli A. , Rainoldi S. , Albanese C. , Santocanale C.
      J. Biol. Chem.282:208-215(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-40 AND SER-53
    17. 17.
      "Identification and characterization of a novel component of the human minichromosome maintenance complex."
      Sakwe A.M. , Nguyen T. , Athanasopoulos V. , Shire K. , Frappier L.
      Mol. Cell. Biol.27:3044-3055(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN THE MCM2-7 COMPLEX;IDENTIFICATION BY MASS SPECTROMETRY
    18. 18.
      "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
      Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
      Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Embryonic kidney.
    19. 19.
      "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
      J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    20. 20.
      "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
      Carrascal M. , Ovelleiro D. , Casas V. , Gay M. , Abian J.
      J. Proteome Res.7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: T-cell.
    21. 21.
      "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
      Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    22. 22.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; THR-39; SER-40; SER-41; SER-53 AND SER-139;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    23. 23.
      "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
    24. 24.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27; THR-59; SER-108 AND SER-139;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
    25. 25.
      "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-216;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    26. 26.
      "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-27; SER-41; SER-108 AND SER-139;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    27. 27.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    28. 28.
      "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
      Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13; SER-26; SER-27; SER-41; SER-139 AND SER-381;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    29. 29.
      "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
      Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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