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Index > Protein center > Mif(Gene name) > Mouse
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  • Mif (Gene name),
  • Macrophage migration inhibitory factor (Protein name ),  MIF_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (3)
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  • Gene name:
    Mif;
    Protein name:
    Macrophage migration inhibitory factor(MIF);
    Alternative:
    Glycosylation-inhibiting factor(GIF);Delayed early response protein 6(DER6);5.3.3.12;L-dopachrome tautomerase);L-dopachrome isomerase;Phenylpyruvate tautomerase;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain.
    Function:
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
    Subcellular Location:
    Secreted Cytoplasm Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
    Protein Attributes:
    Sequence length:
    115
    Sequence:
    50:
    MPMFIVNTNV | PRASVPEGFL | SELTQQLAQA | TGKPAQYIAV | HVVPDQLMTF | 
    100:
    SGTNDPCALC | SLHSIGKIGG | AQNRNYSKLL | CGLLSDRLHI | SPDRVYINYY | 
    115:
    DMNAANVGWN | GSTFA
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    KEGG:
    SMR:
    UniGene:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse MIF
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    E0698p
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    ELISA Kit for Mouse MIF
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    E0698b
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    Packing:
    96T
    ELISA Kit for Mouse MIF
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    E0698r
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    ELISA Kit for Mouse MIF
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    E0698h
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    96T
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    ELISA Kit for Mouse MIF
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    E0698m
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    96T
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    ELISA Kit for Mouse MIF
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    E0698c
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    Packing:
    96T
    CLIA Kit for Mouse MIF
    Cat.:
    U0698b
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    Packing:
    96T
    CLIA Kit for Mouse MIF
    Cat.:
    U0698m
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    MSDS:
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    96T
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    CLIA Kit for Mouse MIF
    Cat.:
    U0698p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse MIF
    Cat.:
    U0698h
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Mouse MIF
    Cat.:
    U0698c
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse MIF
    Cat.:
    U0698r
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Mouse MIF
    Polyclonal Antibody for Mouse MIF
    Cat.:
    P0698Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse MIF
    Cat.:
    P0698Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse MIF
    Polyclonal Antibody for Mouse MIF
    Cat.:
    P0698Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Monoclonal Antibody for Mouse MIF
    Protein for Mouse MIF
    Protein for Mouse MIF
    Cat.:
    R0698m
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Mouse MIF
    Cat.:
    R0698h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Mouse MIF
    Protein for Mouse MIF
    Protein for Mouse MIF

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    12% SDS-PAGE analysis

           12% SDS-PAGE analysis

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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia."
      Bernhagen J. , Calandra T. , Mitchell R.A. , Martin S.B. , Tracey K.J. , Voelter W. , Manogue K.R. , Cerami A. , Bucala R.
      Nature365:756-759(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 2-28
      tissue: Pituitary.
    2. 2.
      "Growth factor-induced delayed early response genes."
      Lanahan A.A. , Williams J.B. , Sanders L.K. , Nathans D.
      Mol. Cell. Biol.12:3919-3929(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: BALB/c.
    3. 3.
      "Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor."
      Mikayama T. , Nakano T. , Gomi H. , Nakagawa Y. , Liu Y.C. , Iwamatsu A. , Weiser W.Y. , Ishizaka K. , Sato M. , Ishii Y.
      Proc. Natl. Acad. Sci. U.S.A.90:10056-10060(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];SUBCELLULAR LOCATION
    4. 4.
      "Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF)."
      Mitchell R. , Bacher M. , Bernhagen J. , Pushkarskaya T. , Seldin M.F. , Bucala R.
      J. Immunol.154:3863-3870(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: 129/Sv.
    5. 5.
      "Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes."
      Bozza M. , Kolakowski L.F. Jr. , Jenkins N.A. , Gilbert D.J. , Copeland N.G. , David J.R. , Gerard C.
      Genomics27:412-419(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: 129/Sv.
    6. 6.
      "Genomic cloning of mouse MIF (macrophage inhibitory factor) and genetic mapping of the human and mouse expressed gene and nine mouse pseudogenes."
      Kozak C.A. , Adamson M.C. , Buckler C.E. , Segovia L. , Paralkar V. , Wistow G.
      Genomics27:405-411(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: 129/Sv.
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: FVB/N.
      tissue: Kidney.
      tissue: Mammary gland.
    8. 8.
      Lubec G. , Klug S.
      Submitted (2007-03) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-12;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Hippocampus.
    9. 9.
      "A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens."
      Wistow G.J. , Shaughnessy M. , Lee D.C. , Hodin J. , Zelenka P.S.
      Proc. Natl. Acad. Sci. U.S.A.90:1272-1275(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-115
      tissue: Lens.
    10. 10.
      "Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF)."
      Bernhagen J. , Mitchell R.A. , Calandra T. , Voelter W. , Cerami A. , Bucala R.
      Biochemistry33:14144-14155(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
    11. 11.
      "Alternative chemical modifications reverse the binding orientation of a pharmacophore scaffold in the active site of macrophage migration inhibitory factor."
      Crichlow G.V. , Cheng K.F. , Dabideen D. , Ochani M. , Aljabari B. , Pavlov V.A. , Miller E.J. , Lolis E. , Al-Abed Y.
      J. Biol. Chem.282:23089-23095(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;ROLE OF TAUTOMERASE INHIBITORS IN IMPROVED SURVIVAL IN CASE OF SEPSIS
    12. 12.
      "A tautomerase-null macrophage migration-inhibitory factor (MIF) gene knock-in mouse model reveals that protein interactions and not enzymatic activity mediate MIF-dependent growth regulation."
      Fingerle-Rowson G. , Kaleswarapu D.R. , Schlander C. , Kabgani N. , Brocks T. , Reinart N. , Busch R. , Schuetz A. , Lue H. , Du X. , Liu A. , Xiong H. , Chen Y. , Nemajerova A. , Hallek M. , Bernhagen J. , Leng L. , Bucala R.
      Mol. Cell. Biol.29:1922-1932(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CD74; CXCR2 AND COPS5;CATALYTIC ACTIVITY;MUTAGENESIS OF PRO-2
    13. 13.
      "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J. , Chen Y. , Tishkoff D.X. , Peng C. , Tan M. , Dai L. , Xie Z. , Zhang Y. , Zwaans B.M. , Skinner M.E. , Lombard D.B. , Zhao Y.
      Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-78;SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-78;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Embryonic fibroblast.
    14. 14.
      "Crystal structure of macrophage migration inhibitory factor complexed with (E)-2-fluoro-p-hydroxycinnamate at 1.8 A resolution: implications for enzymatic catalysis and inhibition."
      Taylor A.B. , Johnson W.H. Jr. , Czerwinski R.M. , Li H.S. , Hackert M.L. , Whitman C.P.
      Biochemistry38:7444-7452(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)
    15. 15.
      "Mechanism of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor: properties of the P1G, P1A, Y95F, and N97A mutants."
      Stamps S.L. , Taylor A.B. , Wang S.C. , Hackert M.L. , Whitman C.P.
      Biochemistry39:9671-9678(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS);TAUTOMERASE ACTIVITY;IDENTIFICATION BY MASS SPECTROMETRY;CLEAVAGE OF INITIATOR METHIONINE;MUTAGENESIS OF PRO-2
    16. 16.
      "Inactivation of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor by 2-oxo-4-phenyl-3-butynoate."
      Golubkov P.A. , Johnson W.H. Jr. , Czerwinski R.M. , Person M.D. , Wang S.C. , Whitman C.P. , Hackert M.L.
      Bioorg. Chem.34:183-199(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH INHIBITOR;TAUTOMERASE ACTIVITY;IDENTIFICATION BY MASS SPECTROMETRY;SUBUNIT
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