NAGA ELISA Kit for Human(Homo sapiens) | EIAab.comNAGA ELISA Kit for Human(Homo sapiens)

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Index > Protein center > NAGA(Gene name) > Human

NAGA (Gene name),

Alpha-N-acetylgalactosaminidase (Protein name ), NAGAB_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

NAGA;

Protein name:

Alpha-N-acetylgalactosaminidase;

Alternative:

Alpha-galactosidase B;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.

Subcellular Location:

Lysosome

Protein Attributes:

Sequence length:

411

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

411:

YPIKNLEMSQ | Q

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P17050

STRING:

9606.ENSP00000379680

SMR:

P17050

MIM:

104170

KEGG:

hsa:4668

UniGene:

Hs.75372

Pfam:

PF02065

Uniprot:

P17050

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ELISA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

E13422h

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Packing:

96T

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ELISA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

E13422r

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Packing:

96T

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ELISA Kit for Human Alpha-N-acetylgalactosaminidase

MSDS:

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ELISA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

E13422m

Price:

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MSDS:

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Packing:

96T

ELISA Kit for Human Alpha-N-acetylgalactosaminidase

MSDS:

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CLIA Kit for Human Alpha-N-acetylgalactosaminidase

MSDS:

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CLIA Kit for Human Alpha-N-acetylgalactosaminidase

MSDS:

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CLIA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

U13422r

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

U13422h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human Alpha-N-acetylgalactosaminidase

Cat.:

U13422m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Alpha-N-acetylgalactosaminidase

Monoclonal Antibody for Human Alpha-N-acetylgalactosaminidase

Protein for Human Alpha-N-acetylgalactosaminidase

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-105
Plasma	98	93-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	91-103%	95-106%	92-107%
EDTA plasma(n=5)	89-97%	93-104%	95-105%	93-106%
heparin plasma(n=5)	93-102%	94-107%	101-108%	97-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Human alpha-N-acetylgalactosaminidase-molecular cloning, nucleotide sequence, and expression of a full-length cDNA. Homology with human alpha-galactosidase A suggests evolution from a common ancestral gene."
Wang A.M. , Bishop D.F. , Desnick R.J.
J. Biol. Chem.265:21859-21866(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
tissue: Lung.
2.

"Structural organization and complete sequence of the human alpha-N-acetylgalactosaminidase gene: homology with the alpha-galactosidase A gene provides evidence for evolution from a common ancestral gene."
Wang A.M. , Desnick R.J.
Genomics10:133-142(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Molecular cloning of a full-length cDNA for human alpha-N-acetylgalactosaminidase (alpha-galactosidase B)."
Tsuji S. , Yamauchi T. , Hiraiwa M. , Isobe T. , Okuyama T. , Sakimura K. , Takahashi Y. , Nishizawa M. , Uda Y. , Miyatake T.
Biochem. Biophys. Res. Commun.163:1498-1504(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Placenta.
4.

"Molecular cloning of two species of cDNAs for human alpha-N-acetylgalactosaminidase and expression in mammalian cells."
Yamauchi T. , Hiraiwa M. , Kobayashi H. , Uda Y. , Miyatake T. , Tsuji S.
Biochem. Biophys. Res. Commun.170:231-237(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E. , Wright C.L. , Edwards C.A. , Davis M.P. , Grinham J.A. , Cole C.G. , Goward M.E. , Aguado B. , Mallya M. , Mokrab Y. , Huckle E.J. , Beare D.M. , Dunham I.
Genome Biol.5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
6.

"The DNA sequence of human chromosome 22."
Dunham I. , Hunt A.R. , Collins J.E. , Bruskiewich R. , Beare D.M. , Clamp M. , Smink L.J. , Ainscough R. , Almeida J.P. , Babbage A.K. , Bagguley C. , Bailey J. , Barlow K.F. , Bates K.N. , Beasley O.P. , Bird C.P. , Blakey S.E. , Bridgeman A.M. , more...
Nature402:489-495(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Placenta.
8.

"Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor."
Warner T.G. , Louie A. , Potier M.
Biochem. Biophys. Res. Commun.173:13-19(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-37
tissue: Placenta.
9.

"Degradation of blood group A glycolipid A-6-2 by normal and mutant human skin fibroblasts."
Asfaw B. , Schindler D. , Ledvinova J. , Cerny B. , Smid F. , Conzelmann E.
J. Lipid Res.39:1768-1780(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
10.

"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction."
Tang L.-Y. , Deng N. , Wang L.-S. , Dai J. , Wang Z.-L. , Jiang X.-S. , Li S.-J. , Li L. , Sheng Q.-H. , Wu D.-Q. , Li L. , Zeng R.
Mol. Cell. Proteomics6:1952-1967(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-322 AND SER-332;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
11.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-177 AND ASN-201
tissue: Liver.
12.

"The 1.9 A structure of human alpha-N-acetylgalactosaminidase: the molecular basis of Schindler and Kanzaki diseases."
Clark N.E. , Garman S.C.
J. Mol. Biol.393:435-447(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 18-411 IN COMPLEXES WITH N-ACETYLGALACTOSAMINE AND GALACTOSE;DISULFIDE BONDS;GLYCOSYLATION AT ASN-124; ASN-177 AND ASN-385;SUBUNIT;CATALYTIC ACTIVITY;MUTAGENESIS OF ASN-201
13.

"Schindler disease: the molecular lesion in the alpha-N-acetylgalactosaminidase gene that causes an infantile neuroaxonal dystrophy."
Wang A.M. , Schindler D. , Desnick R.J.
J. Clin. Invest.86:1752-1756(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT SCHIND LYS-325
14.

"The molecular lesion in the alpha-N-acetylgalactosaminidase gene that causes angiokeratoma corporis diffusum with glycopeptiduria."
Wang A.M. , Kanzaki T. , Desnick R.J.
J. Clin. Invest.94:839-845(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT KANZD TRP-329;CHARACTERIZATION OF VARIANT KANZD TRP-329
15.

"Human alpha-N-acetylgalactosaminidase (alpha-NAGA) deficiency: new mutations and the paradox between genotype and phenotype."
Keulemans J.L.M. , Reuser A.J.J. , Kroos M.A. , Willemsen R. , Hermans M.M.P. , van den Ouweland A.M.W. , de Jong J.G.N. , Wevers R.A. , Renier W.O. , Schindler D. , Coll M.J. , Chabas A. , Sakuraba H. , Suzuki Y. , van Diggelen O.P.
J. Med. Genet.33:458-464(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS SCHIND CYS-160 AND LYS-325
16.

"A new case of alpha-N-acetylgalactosaminidase deficiency with angiokeratoma corporis diffusum, with Meniere's syndrome and without mental retardation."
Kodama K. , Kobayashi H. , Abe R. , Ohkawara A. , Yoshii N. , Yotsumoto S. , Fukushige T. , Nagatsuka Y. , Hirabayashi Y. , Kanzaki T.
Br. J. Dermatol.144:363-368(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT KANZD GLN-329

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