NDRG1 ELISA Kit for Human(Homo sapiens) | EIAab.comNDRG1 ELISA Kit for Human(Homo sapiens) | EIAab.com

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NDRG1 (Gene name),

Protein NDRG1 (Protein name ), NDRG1_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

NDRG1(CAP43;DRG1;RTP);

Protein name:

Protein NDRG1;

Alternative:

N-myc downstream-regulated gene 1 protein;Differentiation-related gene 1 protein(DRG-1);Reducing agents and tunicamycin-responsive protein(RTP);Nickel-specific induction protein Cap43;Rit42;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

May have a growth inhibitory role.

Subcellular Location:

Cytoplasm Nucleus Cell membrane Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.

Protein Attributes:

Sequence length:

394

Sequence:

50:

MSREMQDVDL | AEVKPLVEKG | ETITGLLQEF | DVQEQDIETL | HGSVHVTLCG |

100:

TPKGNRPVIL | TYHDIGMNHK | TCYNPLFNYE | DMQEITQHFA | VCHVDAPGQQ |

150:

DGAASFPAGY | MYPSMDQLAE | MLPGVLQQFG | LKSIIGMGTG | AGAYILTRFA |

200:

LNNPEMVEGL | VLINVNPCAE | GWMDWAASKI | SGWTQALPDM | VVSHLFGKEE |

250:

MQSNVEVVHT | YRQHIVNDMN | PGNLHLFINA | YNSRRDLEIE | RPMPGTHTVT |

300:

LQCPALLVVG | DSSPAVDAVV | ECNSKLDPTK | TTLLKMADCG | GLPQISQPAK |

350:

LAEAFKYFVQ | GMGYMPSASM | TRLMRSRTAS | GSSVTSLDGT | RSRSHTSEGT |

394:

RSRSHTSEGT | RSRSHTSEGA | HLDITPNSGA | AGNSAGPKSM | EVSC

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

KEGG:

SMR:

STRING:

9606.ENSP00000319977

MIM:

UniGene:

String:

Uniprot:

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ELISA Kit for Human Protein NDRG1

Cat.:

E14498h

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96T

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ELISA Kit for Human Protein NDRG1

MSDS:

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ELISA Kit for Human Protein NDRG1

MSDS:

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ELISA Kit for Human Protein NDRG1

Cat.:

E14498m

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Packing:

96T

CLIA Kit for Human Protein NDRG1

Cat.:

U14498m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human Protein NDRG1

MSDS:

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CLIA Kit for Human Protein NDRG1

MSDS:

Please sign in first.

CLIA Kit for Human Protein NDRG1

Cat.:

U14498h

Price:

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Manual:

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MSDS:

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Packing:

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Polyclonal Antibody for Human Protein NDRG1

Polyclonal Antibody for Human Protein NDRG1

Polyclonal Antibody for Human Protein NDRG1

Polyclonal Antibody for Human Protein NDRG1

Monoclonal Antibody for Human Protein NDRG1

Monoclonal Antibody for Human Protein NDRG1

Monoclonal Antibody for Human Protein NDRG1

Monoclonal Antibody for Human Protein NDRG1

Protein for Human Protein NDRG1

Protein for Human Protein NDRG1

Protein for Human Protein NDRG1

Protein for Human Protein NDRG1

R&D Technical Data

s

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

Recovery was determined by spiking various levels of NDRG1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	91-108
Plasma	102	93-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NDRG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	95-114%	97-110%	100-109%
EDTA plasma(n=5)	93-110%	96-109%	94-109%	95-110%
heparin plasma(n=5)	95-108%	94-110%	96-106%	98-105%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes."
Kokame K. , Kato H. , Miyata T.
J. Biol. Chem.271:29659-29665(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INDUCTION;TISSUE SPECIFICITY
tissue: Umbilical vein endothelial cell.
2.

"A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms."
van Belzen N. , Dinjens W.N.M. , Diesveld M.P.G. , Groen N.A. , van der Made A.C.J. , Nozawa Y. , Vlietstra R. , Trapman J. , Bosman F.T.
Lab. Invest.77:85-92(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INDUCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
3.

"Cap43, a novel gene specifically induced by Ni2+ compounds."
Zhou D. , Salnikow K. , Costa M.
Cancer Res.58:2182-2189(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY;INDUCTION
tissue: Lung.
4.

"Differential expression of the RTP/Drg1/Ndr1 gene product in proliferating and growth arrested cells."
Piquemal D. , Joulia D. , Balaguer P. , Basset A. , Marti J. , Commes T.
Biochim. Biophys. Acta1450:364-373(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);INDUCTION
5.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
6.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3)
tissue: Brain.
tissue: Tongue.
7.

"DNA sequence and analysis of human chromosome 8."
Nusbaum C. , Mikkelsen T.S. , Zody M.C. , Asakawa S. , Taudien S. , Garber M. , Kodira C.D. , Schueler M.G. , Shimizu A. , Whittaker C.A. , Chang J.L. , Cuomo C.A. , Dewar K. , FitzGerald M.G. , Yang X. , Allen N.R. , Anderson S. , Asakawa T. , more...
Nature439:331-335(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Kidney.
10.

Angelicheva D. , Kalaydjieva L.
Submitted (2000-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-21 (ISOFORM 1)
tissue: Brain.
11.

Lubec G. , Chen W.-Q. , Sun Y.
Submitted (2008-12) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 4-19; 54-70; 133-148; 199-212; 286-300; 307-322; 328-341 AND 364-388;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Fetal brain cortex.
12.

"Inhibition of tumor cell growth by RTP/rit42 and its responsiveness to p53 and DNA damage."
Kurdistani S.K. , Arizti P. , Reimer C.L. , Sugrue M.M. , Aaronson S.A. , Lee S.W.
Cancer Res.58:4439-4444(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION
13.

"N-myc downstream-regulated gene 1 is mutated in hereditary motor and sensory neuropathy-Lom."
Kalaydjieva L. , Gresham D. , Gooding R. , Heather L. , Baas F. , de Jonge R. , Blechschmidt K. , Angelicheva D. , Chandler D. , Worsley P. , Rosenthal A. , King R.H.M. , Thomas P.K.
Am. J. Hum. Genet.67:47-58(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN CMT4D
14.

"Phosphorylation of RTP, an ER stress-responsive cytoplasmic protein."
Agarwala K.L. , Kokame K. , Kato H. , Miyata T.
Biochem. Biophys. Res. Commun.272:641-647(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;SUBCELLULAR LOCATION
15.

"Expression of NDRG1, a differentiation-related gene, in human tissues."
Lachat P. , Shaw P. , Gebhard S. , van Belzen N. , Chaubert P. , Bosman F.T.
Histochem. Cell Biol.118:399-408(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;TISSUE SPECIFICITY
16.

"Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3."
Murray J.T. , Campbell D.G. , Morrice N. , Auld G.C. , Shpiro N. , Marquez R. , Peggie M. , Bain J. , Bloomberg G.B. , Grahammer F. , Lang F. , Wulff P. , Kuhl D. , Cohen P.
Biochem. J.384:477-488(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-328; SER-330; THR-346; THR-356 AND THR-366
17.

"Function of Drg1/Rit42 in p53-dependent mitotic spindle checkpoint."
Kim K.T. , Ongusaha P.P. , Hong Y.K. , Kurdistani S.K. , Nakamura M. , Lu K.P. , Lee S.W.
J. Biol. Chem.279:38597-38602(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
18.

"NDRG1 is necessary for p53-dependent apoptosis."
Stein S. , Thomas E.K. , Herzog B. , Westfall M.D. , Rocheleau J.V. , Jackson R.S. II , Wang M. , Liang P.
J. Biol. Chem.279:48930-48940(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
19.

"NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24."
Hunter M. , Angelicheva D. , Tournev I. , Ingley E. , Chan D.C. , Watts G.F. , Kremensky I. , Kalaydjieva L.
Biochem. Biophys. Res. Commun.332:982-992(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH APOA1; APOA2; PRA1 AND RTN1;POSSIBLE FUNCTION
20.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330 AND SER-333;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
22.

"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Giorgianni F. , Zhao Y. , Desiderio D.M. , Beranova-Giorgianni S.
Electrophoresis28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Prostate cancer.
23.

"Hypoxia increases cytoplasmic expression of NDRG1, but is insufficient for its membrane localization in human hepatocellular carcinoma."
Sibold S. , Roh V. , Keogh A. , Studer P. , Tiffon C. , Angst E. , Vorburger S.A. , Weimann R. , Candinas D. , Stroka D.
FEBS Lett.581:989-994(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INDUCTION
24.

"The N-Myc down regulated Gene1 (NDRG1) is a Rab4a effector involved in vesicular recycling of E-cadherin."
Kachhap S.K. , Faith D. , Qian D.Z. , Shabbeer S. , Galloway N.L. , Pili R. , Denmeade S.R. , DeMarzo A.M. , Carducci M.A.
PLoS ONE2:E844-E844(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RAB4A;SUBCELLULAR LOCATION;FUNCTION
25.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; SER-330; SER-333; SER-336; SER-364 AND THR-375;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
26.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS];IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
27.

"SGK1 activity in Na+ absorbing airway epithelial cells monitored by assaying NDRG1-Thr346/356/366 phosphorylation."
Inglis S.K. , Gallacher M. , Brown S.G. , McTavish N. , Getty J. , Husband E.M. , Murray J.T. , Wilson S.M.
Pflugers Arch.457:1287-1301(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-346; THR-356 AND THR-366
28.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-328 AND SER-330;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
29.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-333;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
30.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
31.

"Phosphorylation of NDRG1 is temporally and spatially controlled during the cell cycle."
McCaig C. , Potter L. , Abramczyk O. , Murray J.T.
Biochem. Biophys. Res. Commun.411:227-234(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;SUBCELLULAR LOCATION

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