LCN2 ELISA Kit for Human(Homo sapiens) | EIAab.comLCN2 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > LCN2(Gene name) > Human

LCN2 (Gene name),

Neutrophil gelatinase-associated lipocalin (Protein name ), NGAL_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

LCN2(HNL;NGAL);

Protein name:

Neutrophil gelatinase-associated lipocalin(NGAL);

Alternative:

Lipocalin-2;25 kDa alpha-2-microglobulin-related subunit of MMP-9;p25;Oncogene 24p3;p25;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer; disulfide-linked. Heterodimer; disulfide-linked with MMP9.

Function:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

Subcellular Location:

Secreted Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

Protein Attributes:

Sequence length:

198

Sequence:

50:

100:

150:

198:

EYFKITLYGR | TKELTSELKE | NFIRFSKSLG | LPENHIVFPV | PIDQCIDG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000277480

SMR:

P80188

Pfam:

PF00061

KEGG:

hsa:3934

MIM:

600181

String:

P80188

UniGene:

Hs.204238

Uniprot:

P80188

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ELISA Kit for Human NGAL

Cat.:

E1388h

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96T

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ELISA Kit for Human NGAL

Cat.:

E1388r

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96T

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ELISA Kit for Human NGAL

Cat.:

E1388m

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96T

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CLIA Kit for Human NGAL

Cat.:

U1388r

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MSDS:

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Packing:

96T

CLIA Kit for Human NGAL

Cat.:

U1388h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Human NGAL

Cat.:

U1388m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human NGAL

Cat.:

P1388Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human NGAL

Cat.:

P1388Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human NGAL

Cat.:

P1388Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human NGAL

Cat.:

M1388h

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Packing:

40ug

Monoclonal Antibody for Human NGAL

Protein for Human NGAL

Cat.:

R1388h

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Packing:

1mg

Purity:

>90%

Protein for Human NGAL

Cat.:

R1388m

Price:

Please sign in first.

Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	93	87-101
Plasma	90	84-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-105%	99-104%	91-106%	94-108%
heparin plasma(n=5)	101-109%	97-105%	93-99%	91-110%
urine(n=5)	99-103%	97-109%	89-101%	96-108%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"The changes of serum sKlotho and NGAL levels and their correlation in type 2 diabetes mellitus patients with different stages of urinary albumin"

C Wu, Q Wang, C Lv, et al.

From:Diabetes Research and Clinical Practice

References

1.

"Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils."
Bundgaard J.R. , Sengelov H. , Borregaard N. , Kjeldsen L.
Biochem. Biophys. Res. Commun.202:1468-1475(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans."
Cowland J.B. , Borregaard N.
Genomics45:17-23(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];TISSUE SPECIFICITY
tissue: Bone marrow.
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Esophagus.
4.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
5.

"DNA sequence and analysis of human chromosome 9."
Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Pancreas.
8.

"Cloning and expression of human neutrophil lipocalin cDNA derived from bone marrow and ovarian cancer cells."
Bartsch S. , Tschesche H.
FEBS Lett.357:255-259(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-198;PARTIAL PROTEIN SEQUENCE
9.

"Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase."
Kjeldsen L. , Johnsen A.H. , Sengelov H. , Borregaard N.
J. Biol. Chem.268:10425-10432(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-198;PYROGLUTAMATE FORMATION AT GLN-21
tissue: Neutrophil.
10.

"A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase."
Triebel S. , Blaeser J. , Reinke H. , Tschesche H.
FEBS Lett.314:386-388(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 51-61; 71-90; 132-136; 152-160 AND 178-192
tissue: Neutrophil.
11.

"An iron delivery pathway mediated by a lipocalin."
Yang J. , Goetz D. , Li J.Y. , Wang W. , Mori K. , Setlik D. , Du T. , Erdjument-Bromage H. , Tempst P. , Strong R. , Barasch J.
Mol. Cell10:1045-1056(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;IRON-BINDING;SIDEROPHORE-BINDING;SUBCELLULAR LOCATION
12.

"A proteomic analysis of human bile."
Kristiansen T.Z. , Bunkenborg J. , Gronborg M. , Molina H. , Thuluvath P.J. , Argani P. , Goggins M.G. , Maitra A. , Pandey A.
Mol. Cell. Proteomics3:715-728(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-85
tissue: Bile.
13.

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-85
tissue: Plasma.
14.

"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-85
tissue: Saliva.
15.

"Transcription and signalling pathways involved in BCR-ABL-mediated misregulation of 24p3 and 24p3R."
Sheng Z. , Wang S.Z. , Green M.R.
EMBO J.28:866-876(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
16.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-85
tissue: Liver.
17.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"Ligand preference inferred from the structure of neutrophil gelatinase associated lipocalin."
Goetz D.H. , Willie S.T. , Armen R.S. , Bratt T. , Borregaard N. , Strong R.K.
Biochemistry39:1935-1941(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 21-298
19.

"The solution structure and dynamics of human neutrophil gelatinase-associated lipocalin."
Coles M. , Diercks T. , Muehlenweg B. , Bartsch S. , Zolzer V. , Tschesche H. , Kessler H.
J. Mol. Biol.289:139-157(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 21-198
20.

"The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition."
Goetz D.H. , Holmes M.A. , Borregaard N. , Bluhm M.E. , Raymond K.N. , Strong R.K.
Mol. Cell10:1033-1043(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 21-198 IN COMPLEX WITH SIDEROPHORE AND IRON;SUBUNIT

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