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Index > Protein center > Ngb(Gene name) > Mouse
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  • Ngb (Gene name),
  • Neuroglobin (Protein name ),  NGB_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    Ngb;
    Protein name:
    Neuroglobin;
    Alternative:

    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer and homotetramer; disulfide-linked.
    Function:
    Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes.
    Subcellular Location:
    Perikaryon
    Protein Attributes:
    Sequence length:
    151
    Sequence:
    50:
    MERPESELIR | QSWRVVSRSP | LEHGTVLFAR | LFALEPSLLP | LFQYNGRQFS | 
    100:
    SPEDCLSSPE | FLDHIRKVML | VIDAAVTNVE | DLSSLEEYLT | SLGRKHRAVG | 
    150:
    VRLSSFSTVG | ESLLYMLEKC | LGPDFTPATR | TAWSRLYGAV | VQAMSRGWDG | 
    151:
    E
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    KEGG:
    SMR:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Neuroglobin
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    E0606h
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    96T
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    ELISA Kit for Mouse Neuroglobin
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    ELISA Kit for Mouse Neuroglobin
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    E0606d
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    Packing:
    96T
    ELISA Kit for Mouse Neuroglobin
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    E0606p
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    ELISA Kit for Mouse Neuroglobin
    Cat.:
    E0606b
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    96T
    ELISA Kit for Mouse Neuroglobin
    Cat.:
    E0606r
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    96T
    ELISA Kit for Mouse Neuroglobin
    Cat.:
    E0606m
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Neuroglobin
    Cat.:
    U0606d
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Neuroglobin
    Cat.:
    U0606p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Neuroglobin
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    Please sign in first.
    CLIA Kit for Mouse Neuroglobin
    Cat.:
    U0606r
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    Packing:
    96T
    CLIA Kit for Mouse Neuroglobin
    Cat.:
    U0606h
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Mouse Neuroglobin
    Cat.:
    U0606b
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Mouse Neuroglobin
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    U0606m
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Mouse Neuroglobin
    Cat.:
    P0606Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Neuroglobin
    Cat.:
    P0606Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Neuroglobin
    Cat.:
    P0606Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Neuroglobin
    Polyclonal Antibody for Mouse Neuroglobin
    Polyclonal Antibody for Mouse Neuroglobin
    Polyclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Monoclonal Antibody for Mouse Neuroglobin
    Protein for Mouse Neuroglobin
    Cat.:
    R0606h
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Mouse Neuroglobin
    Protein for Mouse Neuroglobin
    Protein for Mouse Neuroglobin
    Protein for Mouse Neuroglobin
    Protein for Mouse Neuroglobin
    Protein for Mouse Neuroglobin

    R&D Technical Data

                 Western blot- anti-NGB antibody

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 6.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 9.8%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    94-106

    Plasma

    99

    92-104

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    94-106%

    95-105%

    93-103%

    90-110%

    EDTA plasma(n=5)

    90-99%

    93-107%

    95-104%

    96-108%

    heparin plasma(n=5)

    94-104%

     

    96-110%

    99-111%

    93-108%

     

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    References
    1. 1.
      "A vertebrate globin expressed in the brain."
      Burmester T. , Weich B. , Reinhardt S. , Hankeln T.
      Nature407:520-522(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Brain.
    2. 2.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Liver.
    3. 3.
      "The heme environment of mouse neuroglobin. Evidence for the presence of two conformations of the heme pocket."
      Couture M. , Burmester T. , Hankeln T. , Rousseau D.L.
      J. Biol. Chem.276:36377-36382(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    4. 4.
      "Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family."
      Dewilde S. , Kiger L. , Burmester T. , Hankeln T. , Baudin-Creuza V. , Aerts T. , Marden M.C. , Caubergs R. , Moens L.
      J. Biol. Chem.276:38949-38955(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;QUATERNARY STRUCTURE;MUTAGENESIS OF HIS-64
    5. 5.
      "Mitochondrial distribution of neuroglobin and its response to oxygen-glucose deprivation in primary-cultured mouse cortical neurons."
      Yu Z. , Xu J. , Liu N. , Wang Y. , Li X. , Pallast S. , van Leyen K. , Wang X.
      Neuroscience218:235-242(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    6. 6.
      "The structure of carbonmonoxy neuroglobin reveals a heme-sliding mechanism for control of ligand affinity."
      Vallone B. , Nienhaus K. , Matthes A. , Brunori M. , Nienhaus G.U.
      Proc. Natl. Acad. Sci. U.S.A.101:17351-17356(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH CARBON MONOXIDE
    7. 7.
      "The structure of murine neuroglobin: Novel pathways for ligand migration and binding."
      Vallone B. , Nienhaus K. , Brunori M. , Nienhaus G.U.
      Proteins56:85-92(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN A HEXACOORDINATED FE(3+)-BOUND FORM
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