Neuroglobin (Protein name
), NGB_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
Ngb;
Protein name:
Neuroglobin;
Alternative:
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Monomer (By similarity). Homodimer and homotetramer; disulfide-linked.
Function:
Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 6.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 9.8%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
101
94-106
Plasma
99
92-104
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-106%
95-105%
93-103%
90-110%
EDTA plasma(n=5)
90-99%
93-107%
95-104%
96-108%
heparin plasma(n=5)
94-104%
96-110%
99-111%
93-108%
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