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Index > Protein center > Ngb(Gene name) > Rat
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  • Ngb (Gene name),
  • Neuroglobin (Protein name ),  NGB_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Ngb;
    Protein name:
    Neuroglobin;
    Alternative:

    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Monomer (By similarity). Homodimer and homotetramer; disulfide-linked.
    Function:
    Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes.
    Subcellular Location:
    Perikaryon
    Protein Attributes:
    Sequence length:
    151
    Sequence:
    50:
    MERPESELIR | QSWRAVSRSP | LEHGTVLFSR | LFALEPSLLP | LFQYNGRQFS | 
    100:
    SPEDCLSSPE | FLDHIRKVML | VIDAAVTNVE | DLSSLEEYLA | TLGRKHRAVG | 
    150:
    VRLSSFSTVG | ESLLYMLEKC | LGPDFTPATR | TAWSQLYGAV | VQAMSRGWDG | 
    151:
    E
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    String:
    KEGG:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606h
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    96T
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    ELISA Kit for Rat Neuroglobin
    MSDS:
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    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606d
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    Packing:
    96T
    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606p
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606b
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606r
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    96T
    ELISA Kit for Rat Neuroglobin
    Cat.:
    E0606m
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606d
    Price:
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606p
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Neuroglobin
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606r
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606h
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606b
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Neuroglobin
    Cat.:
    U0606m
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Neuroglobin
    Cat.:
    P0606Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Neuroglobin
    Cat.:
    P0606Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Neuroglobin
    Cat.:
    P0606Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Neuroglobin
    Polyclonal Antibody for Rat Neuroglobin
    Polyclonal Antibody for Rat Neuroglobin
    Polyclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Monoclonal Antibody for Rat Neuroglobin
    Protein for Rat Neuroglobin
    Cat.:
    R0606h
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Rat Neuroglobin
    Protein for Rat Neuroglobin
    Protein for Rat Neuroglobin
    Protein for Rat Neuroglobin
    Protein for Rat Neuroglobin
    Protein for Rat Neuroglobin

    R&D Technical Data

                 Western blot- anti-NGB antibody

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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 6.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 9.8%

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    Recovery

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    94-106

    Plasma

    99

    92-104

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    94-106%

    95-105%

    93-103%

    90-110%

    EDTA plasma(n=5)

    90-99%

    93-107%

    95-104%

    96-108%

    heparin plasma(n=5)

    94-104%

     

    96-110%

    99-111%

    93-108%

     

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    References
    1. 1.
      "Coding region cDNA sequence cloning of rat neuroglobin gene, its polymorphism feature and tissue expression profile analysis."
      Zhang C.G. , Li L. , Deng M.Y. , Xie F. , Wang C.L. , Zhou W.Q. , Wang H.Y. , He F.C.
      Yi Chuan Xue Bao28:997-1001(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Wistar.
    2. 2.
      "Full-length cDNA cloning of human neuroglobin and tissue expression of rat neuroglobin."
      Zhang C.G. , Wang C.L. , Deng M.Y. , Li L. , Wang H.Y. , Fan M. , Xu W.L. , Meng F.W. , Qian L. , He F.C.
      Biochem. Biophys. Res. Commun.290:1411-1419(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY
    3. 3.
      "Molecular cloning and sequencing of rat brain neuroglobin."
      Parthasarathy S.N. , Parthasarathy L. , Parthasarathy R.
      Submitted (2001-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Brain.
    4. 4.
      "Rattus norvegicus mRNA for neuroglobin."
      Lee H.-M. , Greeley G.H. , Englander E.W.
      Submitted (2001-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Sprague-Dawley.
      tissue: Cerebellum.
    5. 5.
      "Rat neuroglobin (NGB) cDNA, partial sequence."
      Wang H. , Gao X. , Wang B. , Huang Y. , Han J.
      Submitted (2001-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-119
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