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Index > Protein center > NGF(Gene name) > Human
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  • NGF (Gene name),
  • Beta-nerve growth factor (Protein name ),  NGF_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (2)
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  • Gene name:
    NGF(NGFB);
    Protein name:
    Beta-nerve growth factor(Beta-NGF);
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer.
    Function:
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    241
    Sequence:
    50:
    MSMLFYTLIT | AFLIGIQAEP | HSESNVPAGH | TIPQAHWTKL | QHSLDTALRR | 
    100:
    ARSAPAAAIA | ARVAGQTRNI | TVDPRLFKKR | RLRSPRVLFS | TQPPREAADT | 
    150:
    QDLDFEVGGA | APFNRTHRSK | RSSSHPIFHR | GEFSVCDSVS | VWVGDKTTAT | 
    200:
    DIKGKEVMVL | GEVNINNSVF | KQYFFETKCR | DPNPVDSGCR | GIDSKHWNSY | 
    241:
    CTTTHTFVKA | LTMDGKQAAW | RFIRIDTACV | CVLSRKAVRR | A
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    MIM:
    UniGene:
    Pfam:
    KEGG:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Beta-NGF
    Cat.:
    E0105b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Beta-NGF
    Cat.:
    U0105c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Beta-NGF
    Cat.:
    P0105Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Beta-NGF
    Polyclonal Antibody for Human Beta-NGF
    Polyclonal Antibody for Human Beta-NGF
    Cat.:
    P0105Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Beta-NGF
    Polyclonal Antibody for Human Beta-NGF
    Cat.:
    P0105Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Beta-NGF
    Monoclonal Antibody for Human Beta-NGF
    Monoclonal Antibody for Human Beta-NGF
    Monoclonal Antibody for Human Beta-NGF
    Monoclonal Antibody for Human Beta-NGF
    Monoclonal Antibody for Human Beta-NGF
    Protein for Human Beta-NGF
    Protein for Human Beta-NGF
    Protein for Human Beta-NGF
    Protein for Human Beta-NGF
    Protein for Human Beta-NGF
    Protein for Human Beta-NGF

    R&D Technical Data

    s

    s

    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.5%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.0%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.3%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.3%

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    Recovery

    Recovery was determined by spiking various levels of NGF into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    91-100

    Plasma

    98

    95-106

     

     

     

     

    Recovery was determined by spiking various levels of NGF into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    100

    94-105

    Plasma

    101

    98-107

     

     

     

     

    Recovery was determined by spiking various levels of NGF into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    92-103

    Plasma

    98

    97-105

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    89-97%

    92-102%

    100-104%

    91-98%

    EDTA plasma(n=5)

    90-98%

    95-100%

    90-96%

    99-104%

    heparin plasma(n=5)

    95-102%

     

    93-104%

    97-102%

    85-96%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    100-106%

    94-103%

    90-102%

    97-105%

    EDTA plasma(n=5)

    87-94%

    91-99%

    102-107%

    103-110%

    heparin plasma(n=5)

    102-106%

     

    91-96%

    97-104%

    95-106%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-100%

    97-104%

    93-105%

    102-107%

    EDTA plasma(n=5)

    97-101%

    102-105%

    89-94%

    94-103%

    heparin plasma(n=5)

    102-106%

     

    90-97%

    97-103%

    92-105%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Human beta-nerve growth factor gene sequence highly homologous to that of mouse."
      Ullrich A. , Gray A. , Berman C. , Dull T.J.
      Nature303:821-825(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
    2. 2.
      "Sequence homology of human and mouse beta-NGF subunit genes."
      Ullrich A. , Gray A. , Berman C. , Coussens L. , Dull T.J.
      Cold Spring Harb. Symp. Quant. Biol.48:435-442(1983) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
    3. 3.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT VAL-35
      tissue: Brain.
    4. 4.
      "Cloning and sequencing of the gene for premature beta nerve growth factor."
      Tong Y. , Wang H. , Chen W.
      Zhongguo Ying Yong Sheng Li Xue Za Zhi13:316-318(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      "Human-specific amino acid changes found in 103 protein-coding genes."
      Kitano T. , Liu Y.-H. , Ueda S. , Saitou N.
      Mol. Biol. Evol.21:936-944(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT VAL-35
    6. 6.
      Zhang Y. , Zhang B. , Zhou Y. , Peng X. , Yuan J. , Qiang B.
      Submitted (2001-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    7. 7.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A. , Ebert L. , Mkoundinya M. , Schick M. , Eisenstein S. , Neubert P. , Kstrang K. , Schatten R. , Shen B. , Henze S. , Mar W. , Korn B. , Zuo D. , Hu Y. , LaBaer J.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    8. 8.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    9. 9.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    10. 10.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT VAL-35
    11. 11.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT VAL-35
      tissue: Eye.
    12. 12.
      "Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary."
      Hallboeoek F. , Ibanez C.F. , Persson H.
      Neuron6:845-858(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-219
      tissue: Leukocyte.
    13. 13.
      "The trk proto-oncogene encodes a receptor for nerve growth factor."
      Klein R. , Jing S. , Nanduri V. , O'Rourke E. , Barbacid M.
      Cell65:189-197(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF NTRK1 AS THE HIGH AFFINITY NGF RECEPTOR
    14. 14.
      "Crystal structure of nerve growth factor in complex with the ligand-binding domain of the TrkA receptor."
      Wiesmann C. , Ultsch M.H. , Bass S.H. , de Vos A.M.
      Nature401:184-188(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 122-241;DISULFIDE BONDS
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT VAL-35
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
    17. 17.
      "A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception."
      Einarsdottir E. , Carlsson A. , Minde J. , Toolanen G. , Svensson O. , Solders G. , Holmgren G. , Holmberg D. , Holmberg M.
      Hum. Mol. Genet.13:799-805(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HSAN5 TRP-221
    18. 18.
      "A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy."
      Carvalho O.P. , Thornton G.K. , Hertecant J. , Houlden H. , Nicholas A.K. , Cox J.J. , Rielly M. , Al-Gazali L. , Woods C.G.
      J. Med. Genet.48:131-135(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT HSAN5 TRP-221
    19. 19.
      "Frequency of mutations in the genes associated with hereditary sensory and autonomic neuropathy in a UK cohort."
      Davidson G.L. , Murphy S.M. , Polke J.M. , Laura M. , Salih M.A. , Muntoni F. , Blake J. , Brandner S. , Davies N. , Horvath R. , Price S. , Donaghy M. , Roberts M. , Foulds N. , Ramdharry G. , Soler D. , Lunn M.P. , Manji H. , more...
      J. Neurol.259:1673-1685(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HSAN5 GLY-GLU-162 INS;VARIANT ASN-187
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