Ngf ELISA Kit for Mouse(Mus musculus) | EIAab.comNgf ELISA Kit for Mouse(Mus musculus)

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Index > Protein center > Ngf(Gene name) > Mouse

Ngf (Gene name),

Beta-nerve growth factor (Protein name ), NGF_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

Ngf(Ngfb);

Protein name:

Beta-nerve growth factor(Beta-NGF);

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer.

Function:

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

241

Sequence:

50:

100:

150:

200:

241:

CTTTHTFVKA | LTTDEKQAAW | RFIRIDTACV | CVLSRKATRR | G

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Mm.1259

KEGG:

mmu:18049

Pfam:

PF00243

String:

P01139

SMR:

P01139

Uniprot:

P01139

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ELISA Kit for Mouse Beta-NGF

Cat.:

E0105r

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96T

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ELISA Kit for Mouse Beta-NGF

Cat.:

E0105h

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96T

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ELISA Kit for Mouse Beta-NGF

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E0105p

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ELISA Kit for Mouse Beta-NGF

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E0105m

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ELISA Kit for Mouse Beta-NGF

Cat.:

E0105c

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96T

ELISA Kit for Mouse Beta-NGF

Cat.:

E0105b

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96T

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105c

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96T

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105b

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96T

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105h

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Please sign in first.

Packing:

96T

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105m

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Packing:

96T

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105p

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Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

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Please sign in first.

CLIA Kit for Mouse Beta-NGF

Cat.:

U0105r

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Looking for manual.

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Polyclonal Antibody for Mouse Beta-NGF

Cat.:

P0105Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Beta-NGF

Cat.:

P0105Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse Beta-NGF

Cat.:

P0105Rb-r

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40ug/0.2ml

Polyclonal Antibody for Mouse Beta-NGF

Monoclonal Antibody for Mouse Beta-NGF

Protein for Mouse Beta-NGF

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-100
Plasma	98	95-106

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	94-105
Plasma	101	98-107

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	92-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-97%	92-102%	100-104%	91-98%
EDTA plasma(n=5)	90-98%	95-100%	90-96%	99-104%
heparin plasma(n=5)	95-102%	93-104%	97-102%	85-96%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-106%	94-103%	90-102%	97-105%
EDTA plasma(n=5)	87-94%	91-99%	102-107%	103-110%
heparin plasma(n=5)	102-106%	91-96%	97-104%	95-106%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-100%	97-104%	93-105%	102-107%
EDTA plasma(n=5)	97-101%	102-105%	89-94%	94-103%
heparin plasma(n=5)	102-106%	90-97%	97-103%	92-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors"

Fang Z, Zicong L, Qingchun Z, et al.

From:Scientific Reports

References

1.

"Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor."
Scott J. , Selby M.J. , Urdea M.S. , Quiroga M. , Bell G.I. , Rutter W.J.
Nature302:538-540(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Submandibular gland.
2.

"Human beta-nerve growth factor gene sequence highly homologous to that of mouse."
Ullrich A. , Gray A. , Berman C. , Dull T.J.
Nature303:821-825(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Sequence homology of human and mouse beta-NGF subunit genes."
Ullrich A. , Gray A. , Berman C. , Coussens L. , Dull T.J.
Cold Spring Harb. Symp. Quant. Biol.48:435-442(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Mouse nerve growth factor gene: structure and expression."
Selby M.J. , Edwards R. , Sharp F. , Rutter W.J.
Mol. Cell. Biol.7:3057-3064(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: C57BL/6.
tissue: Submandibular gland.
5.

"Production and secretion of nerve growth factor by clonal striated muscle cell line, G8-1."
Yamamoto T. , Yamakuni T. , Okabe N. , Amano T.
Neurochem. Int.21:251-258(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Skeletal muscle.
6.

"Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure."
Angeletti R.H. , Hermodson M.A. , Bradshaw R.A.
Biochemistry12:100-115(1973) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 122-239
7.

"p75 neurotrophin receptor is a clock gene that regulates oscillatory components of circadian and metabolic networks."
Baeza-Raja B. , Eckel-Mahan K. , Zhang L. , Vagena E. , Tsigelny I.F. , Sassone-Corsi P. , Ptacek L.J. , Akassoglou K.
J. Neurosci.33:10221-10234(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
8.

"New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor."
McDonald N.Q. , Lapatto R. , Murray-Rust J. , Gunning J. , Wlodawer A. , Blundell T.L.
Nature354:411-414(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)
9.

"Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites."
Holland D.R. , Cousens L.S. , Meng W. , Matthews B.W.
J. Mol. Biol.239:385-400(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)
10.

"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."
Bax B. , Blundell T.L. , Murray-Rust J. , McDonald N.Q.
Structure5:1275-1285(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX
strain: Swiss Webster.
tissue: Submandibular gland.

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